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    Bepaling van theofylline (1,3-dimethylxanthine) in hondeplasma met behulp van reversed-phase vloeistofchromatografie

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    Authors
    Olling
    M
    Besamusca
    P.
    Rauws
    A.G.
    Type
    Report
    Language
    nl
    
    Metadata
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    Title
    Bepaling van theofylline (1,3-dimethylxanthine) in hondeplasma met behulp van reversed-phase vloeistofchromatografie
    Translated Title
    Determination of theophylline in dog plasma by reversed phase liquid chromatography
    Publiekssamenvatting
    In dit rapport wordt een snelle en nauwkeurige methode beschreven voor de bepaling van theofylline in plasma. Theofylline wordt uit plasma geextraheerd met chloroform/2-propanol en met behulp van reversed-phase hogedruk vloeistofchromatografie gescheiden en met spectrofotometrische detectie bepaald bij 273 nm. De nauwkeurigheid van de methode is 4%, de terugwinning is 100% en de ondergrens 0.1 mg/l plasma. De metabolieten van theofylline en andere xanthinederivaten storen de bepaling niet. Deze methode wordt thans toegepast bij biologische beschikbaarheidsexperimenten van theofylline-preparaten bij de hond.
    Abstract not available
    Sponsors
    RIVM
    URI
    http://hdl.handle.net/10029/255671
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    RIVM official reports

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