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dc.contributor.authorSchets FM
dc.contributor.authorHavelaar AH
dc.date.accessioned2012-12-12T12:29:42Z
dc.date.available2012-12-12T12:29:42Z
dc.date.issued1990-09-30
dc.identifier148608001
dc.identifier.urihttp://hdl.handle.net/10029/255788
dc.description.abstractThe determination of E.coli as a fecal indicator for surface- and drinking water according to NEN 6261 is based on the ability of E.coli to produce indole from tryptophane. E.coli is also able to use the constitutive enzyme beta-glucuronidase to produce the fluorescent methylum-belliferyl from the substrate 4-methylumbelliferyl-beta-D- glucuronide (MUG). This report describes research on the possibility to use the beta-glucuronidase-activity as an identification- characteristic for E.coli. 14% of the isolated E.coli strains was found to be beta-glucuronidase-negative at 44 degr. C. 24% of these strains showed a positive beta-glucuronidase-reaction at 37 degr. C. Two percent of the isolated E.coli strains was indole-negative and four percent of strains other than E.coli gave false positive reactions in this test. There were no false-positive beta-glucuronidase reactions. It is concluded that the indole-reaction gives better results than the beta-glucuronidase-reaction. The interpretation of the beta- glucuronidase- reaction is more difficult and it gives more false- negative results.<br>
dc.description.sponsorshipRIVM
dc.format.extent31 p
dc.language.isonl
dc.publisherRijksinstituut voor Volksgezondheid en Milieu RIVM
dc.relation.ispartofRIVM Rapport 148608001
dc.relation.urlhttp://www.rivm.nl/bibliotheek/rapporten/148608001.html
dc.subject01nl
dc.subjecte.colinl
dc.subjectbeta-glucuronidasenl
dc.subjectmugnl
dc.titleIdentificatie en kwantificering van Escherichia coli door bepaling van de beta-glucuronidase-activiteitnl
dc.title.alternativeIdentification and quantification of Escherichia coli by determination of beta-glucuronidase-activityen
dc.typeOnderzoeksrapport
dc.date.updated2012-12-12T12:29:43Z
html.description.abstractThe determination of E.coli as a fecal indicator for surface- and drinking water according to NEN 6261 is based on the ability of E.coli to produce indole from tryptophane. E.coli is also able to use the constitutive enzyme beta-glucuronidase to produce the fluorescent methylum-belliferyl from the substrate 4-methylumbelliferyl-beta-D- glucuronide (MUG). This report describes research on the possibility to use the beta-glucuronidase-activity as an identification- characteristic for E.coli. 14% of the isolated E.coli strains was found to be beta-glucuronidase-negative at 44 degr. C. 24% of these strains showed a positive beta-glucuronidase-reaction at 37 degr. C. Two percent of the isolated E.coli strains was indole-negative and four percent of strains other than E.coli gave false positive reactions in this test. There were no false-positive beta-glucuronidase reactions. It is concluded that the indole-reaction gives better results than the beta-glucuronidase-reaction. The interpretation of the beta- glucuronidase- reaction is more difficult and it gives more false- negative results.&lt;br&gt;


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