• Login
    View Item 
    •   Home
    • RIVM official reports
    • RIVM official reports
    • View Item
    •   Home
    • RIVM official reports
    • RIVM official reports
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    RIVM Publications RepositoryCommunitiesTitleAuthorsIssue DateSubmit Date

    My Account

    LoginRegister

    Statistics

    Display statistics

    Pilotstudie naar de mogelijkheden van kwaliteitscontrole voor de PCR voor het aantonen van Chlamydia trachomatis voor diagnostiek en surveillance

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Average rating
     
       votes
    Cast your vote
    You can rate an item by clicking the amount of stars they wish to award to this item. When enough users have cast their vote on this item, the average rating will also be shown.
    Star rating
     
    Your vote was cast
    Thank you for your feedback
    Authors
    Ossewaarde JM
    Type
    Report
    Language
    nl
    
    Metadata
    Show full item record
    Title
    Pilotstudie naar de mogelijkheden van kwaliteitscontrole voor de PCR voor het aantonen van Chlamydia trachomatis voor diagnostiek en surveillance
    Translated Title
    [A feasibility study of quality assessment for PCR detection of Chlamydia trachomatis in diagnosis and surveillance.]
    Publiekssamenvatting
    Abstract niet beschikbaar
    A feasibility study on the possibilities of quality assessment for PCR diagnostic tests for detection of Chlamydia trachomatis was carried out among seven laboratories. Fifteen samples from patients, i.e. six culture negative samples and three dilultion's of three culture-positive samples, were mailed to and tested by the laboratories. Four times they used an in house developed PCR test and five times the commercially available AmplicorTM kit. With one exception, all negative samples were correctly reported as negative. One sample was false-positive reported by an in house developed PCR test. Nine times a false-negative result was reported. Five samples were false negative in two of the in house developed PCR tests. One PCR protocol appeared to be sub optimal and one PCR test used as detection method of the amplicon gel electrophoresis only, which is probably not sensitive enough. Three samples were reported false negative by one laboratory using the AmplicorTM kit and one sample by another laboratory. The cause appeared to be an inappropriate method of sample preparation. Using an optimal protocol both in house developed PCR tests and the commercially available AmplicorTM kit appear to be equally sensitive. It was concluded, that PCR quality assessment by mailing selected samples in dilution series is feasible.
    Sponsors
    GHI
    URI
    http://hdl.handle.net/10029/256249
    Collections
    RIVM official reports

    entitlement

     

    DSpace software (copyright © 2002 - 2023)  DuraSpace
    Quick Guide | Contact Us
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.