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    Het ontwikkelen en valideren van een analysemethode voor arseen in urine

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    Authors
    Heerde E van
    Ooik A van
    Ritsema R
    Type
    Report
    Language
    nl
    
    Metadata
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    Title
    Het ontwikkelen en valideren van een analysemethode voor arseen in urine
    Translated Title
    Development and validation of an analytical method for arsenic in urine
    Publiekssamenvatting
    Beschreven is het onderzoek voor het ontwikkelen en valideren van een gevoelige analyse- methode voor de bepaling van het totaal arseengehalte in urine van niet specifiek blootgestelde personen. De metingen zijn verricht met hydride FIA-AAS gebaseerd op vorming van arseenhydrides. Daar alleen anorganisch arseen middels deze techniek gemeten kan worden is een geschikte monstervoorbewerkingsstap nodig voor de omzetting van organisch gebonden arseen in anorganisch arseen. Een UV-destructiemethode is geoptimaliseerd waarna de prestatiekenmerken van de gehele procedure zijn vastgesteld. De aantoonbaarheidsgrens is bepaald op 0,35 mug/l urine terwijl de herhaalbaarheid en de binnenlaboratorium reproduceerbaarheid voor arseengehalten van meer dan 3 mug/l beter is dan respectievelijk 5% en 8% uitgedrukt als relatieve standaarddeviatie. De methode is tevens gevalideerd met gecertificeerde referentiematrialen humane urine. De gemeten resultaten lagen ruim binnen de gecertificeerde intervallen. Naast de analyse van diverse monsters urine met de ontwikkelde methode zijn dezelfde monsters ter controle onderzocht met HPLC / ICP-MS. De arseengehalten gemeten met elk van de methoden verschillen niet significant van elkaar.
    The development and validation of a method for analyzing arsenic in urine samples of non specific exposed persons has been described. Measurements are performed by FIA-AAS based on reduction of inorganic arsenic to arsine. In this way only inorganic arsenic can be quantified. For total arsenic determination in urine a digestion must be performed in order to convert organic-bound arsenic into its inorganic form. A method of UV-photodestruction is optimised followed by establishing the performance characteristics of the entire procedure. The limit of detection is found to be 0.35 mug/l urine, repeatability and within lab reproducibility are better than 5% and 8 % respectively (calculated as relative standard deviation) for arsenic concentrations above 3 mug/l. The method is also validated with certified reference materials of human urine. Results obtained were well within the certified intervals. Several urine samples analysed with the developed method were also analysed with HPLC / ICP-MS to check results. Arsenic concentrations in the urine samples did not differ from each other significantly.
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    IGB
    URI
    http://hdl.handle.net/10029/256625
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