The thirteenth CRL-Salmonella workshop, 26 and 27 May 2008, Bilthoven, the Netherlands
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TitleThe thirteenth CRL-Salmonella workshop, 26 and 27 May 2008, Bilthoven, the Netherlands
Translated TitleDe dertiende CRL-Salmonella workshop, 26 en 27 mei 2008, Bilthoven, Nederland
PubliekssamenvattingOp 26 en 27 mei 2008 vond voor de dertiende keer de jaarlijkse workshop plaats voor de Nationale Referentie Laboratoria (NRL's) voor Salmonella. Het doel van de workshop is informatie uitwisselen over activiteiten van zowel de NRL's als van het Communautair Referentie Laboratorium (CRL) Salmonella. Een belangrijk onderdeel daarvan is de presentatie van de resultaten van de jaarlijks terugkerende ringonderzoeken van het CRL, waarmee de kwaliteit van de NRL-laboratoria wordt gemeten. Ook presenteren de NRL's van enkele geselecteerde landen hoe zij hun taken en verplichtingen uitvoeren.
Dit rapport bevat verslagen van de gehouden presentaties. Veel aandacht ging uit naar de instrumenten om Salmonella aan te tonen. Onder andere kwamen de jaarlijkse 'baseline' studies voor Salmonella aan de orde, waarin per deelnemend land wordt vastgesteld hoeveel Salmonella een bepaald product bevat. Vorig jaar betrof dit kalkoenen en slachtvarkens, dit jaar gaat het om fokvarkens en karkassen van kuikens. Verder zijn meerdere methoden besproken die Salmonella aantonen en typeren. Bijvoorbeeld hoe vastgesteld kan worden of de Salmonella waarvan mensen ziek zijn geworden dezelfde is als die in een product is aangetroffen. Ook zijn verschillende serologische methoden om Salmonella op te sporen, die antistoffen in bloed meten, getoetst op hun werkzaamheid. Hoewel landen hiervoor verschillende methoden gebruiken, hebben de meeste goede resultaten behaald bij het ringonderzoek.
De organisatie van deze workshop is in handen van het CRL voor Salmonella, die op het RIVM is gevestigd. De hoofdtaak van het CRL-Salmonella is toezien op de kwaliteit van de nationale referentielaboratoria voor deze bacterie in Europa. De workshop vond plaats in Bilthoven, Nederland.
The thirteenth annual workshop for the National Reference Laboratories (NRLs) for Salmonella was held on 26-27 May 2008. The aim of this workshop was to facilitate the exchange of information on the activities of the NRLs and the Community Reference Laboratory (CRL) for Salmonella. An important item on the agenda was the presentation of the results of the annual ring trials of the CRL, which provided valuable information on the quality of the participating NRL laboratories. The NRLs of selected countries also described their activities and how they carried these out to meet their responsibilities.
This report contains the summaries of the presentations. These included the presentations of the results of the annual baseline studies for Salmonella, in which each participating country determines the prevalence of Salmonella in certain products. Last year the products under study originated from turkeys and slaughter pigs, while this year the focus is on breeder pigs and broiler carcasses. Methods for detecting and typing of Salmonella were also discussed, including those that are of use in identifying whether the Salmonella that has caused (an outbreak of) illness in humans is the same as that found in a product. The different serological methods used to detect Salmonella have also been tested for their usefulness, and the results were reported at the workshop. Although different methods are used in the Member States, most laboratories obtained good results in the ring trials.
The workshop was organised by the CRL for Salmonella, which is situated at the RIVM. The main task of the CRL-Salmonella is to check the performance of the European National Reference Laboratories for the detection and typing of Salmonella in different products. The workshop was organised in Bilthoven, the Netherlands.
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Thirteenth CRL-Salmonella interlaboratory comparison study (2008) on typing of Salmonella spp. : Dertiende CRL-Salmonella ringonderzoek (2008) voor de typering van Salmonella spp.Berk PA; Maas HME; de Pinna E; Mooijman KA; LZO; cib (Rijksinstituut voor Volksgezondheid en Milieu RIVMHealth Protection Agency HPA, 2010-11-04)The National Reference Laboratories (NRLs) of all 27 European Member States performed well on the 2008 quality control test on Salmonella typing. The 4 laboratories which repeated the test also obtained good scores. An analysis of the pooled results from all NRLs revealed that the NRLs taken as a whole were able to assign the correct name to 97 % of the strains tested. One NRL performed the test at a relatively late date and, consequently, its data could not be included in the group analysis. Since 1992, the NRLs have been required to participate in an annual quality control test, which consists of an interlaboratory comparison study for Salmonella typing. Each Member State designates a specific laboratory within their national boundaries to be responsible for the detection and identification of Salmonella strains from samples isolated from animals and/or food products. These laboratories are then referred to as the National Reference Laboratories. The performance of the NRLs is assessed annually based on their capability to correctly identify 20 Salmonella strains. NRLs from countries outside the European Union occasionally participate in these tests, and NRLs from 2 countries belonging to the European Free Trade Association (EFTA) took part in the 2008 test. The expertise of a number of NRLs was subjected to more severe testing by having not only to identify the 20 Salmonella strains of the quality control test but also to subtype (phage typing) various other Salmonella strains. As such, these laboratories received 10 strains of each of Salmonella Enteritidis and Salmonella Typhimurium. These NRLs typed 97 % of the S. Typhimurium strains correctly. The typing of S. Enteritidis strains proved to be more troublesome, with the NRLs typing 94 % of the strains correctly. The Community Reference Laboratory for Salmonella (CRL-Salmonella) organises this annual interlaboratory comparison study in cooperation with the Health Protection Agency in London, UK. The CRL-Salmonella is situated at the National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands.
Sixteenth EURL-Salmonella interlaboratory comparison study (2011) on typing of Salmonella spp. : Zestiende EURL-Salmonella ringonderzoek (2011) voor de typering van Salmonella spp.Jacobs-Reitsma WF; Pol-Hofstad IE; Maas HME; de Pinna E; Mooijman KA; LZO; cib (Rijksinstituut voor Volksgezondheid en Milieu RIVM, 2012-10-24)The 28 National Reference Laboratories (NRLs) of all 27 European Union (EU) Member States performed well on the 2011 quality control test on Salmonella typing. Two laboratories were found to require a follow-up study on their first test. Altogether, the EU-NRLs were able to assign the correct name to 97% of the strains tested. Other participants interlaboratory comparison study Salmonella Since 1992, the NRLs of the EU Member States have been required to participate in annual quality control tests, which consist of interlaboratory comparison studies on Salmonella. Laboratories from countries outside the European Union, like EU-candidate countries, occasionally participate in these tests on a voluntary basis. Eight additional laboratories participated in the current study. Two EUcandidate countries amongst these eight additional participants did not meet the criteria for good performance in the first round. One of them did not reach this goal in the follow-up study either. The other was not able to participate in the follow-up study; a follow-up study is not compulsory for non-EU laboratories. Each EU Member State designates a specific laboratory within their national boundaries to be responsible for the detection and identification of Salmonella strains from animals and/or food products. These laboratories are then referred to as the National Reference Laboratories. The performance of these NRLs on Salmonella typing is assessed annually, based on their capability to correctly identify 20 Salmonella strains. Phage typing Nine NRLs not only serotyped the 20 Salmonella strains of the quality control test, but also subtyped 20 additional strains by phage typing. For this, the laboratories received ten strains of Salmonella Enteritidis and ten strains of Salmonella Typhimurium. These NRLs typed 98% of the S. Typhimurium strains correctly. Of the S. Enteritidis strains, 88% were phage typed correctly. The European Union Reference Laboratory for Salmonella (EURL-Salmonella) organises this annual interlaboratory comparison study on typing of Salmonella in cooperation with the Health Protection Agency in London, UK. The EURLSalmonella is situated at the National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands.
Test results of Salmonella typing by the NRLs-Salmonella in the Member States of the EU and the EnterNet Laboratories - Collaborative study VI (2001) on typing of SalmonellaKorver H; Raes M; Maas HME; Ward LR; Wannet WJB; Henken AM; MGB; LIS (PHLS-Colindale/London, 2002-09-06)Test results of Salmonella sero- and phage typing and antimicrobial susceptibility testing by the National Reference Laboratories for Salmonella in the Member States of the European Union and the EnterNet Laboratories: Collaborative study VI (2001) for Salmonella. The sixth collaborative typing study for Salmonella was organised by the Community Reference Laboratory for Salmonella (CRL-Salmonella, Bilthoven, The Netherlands) in collaboration with the Public Health Laboratory Services (PHLS), London, UK. Seventeen National Reference Laboratories for Salmonella (NRLs-Salmonella) and 15 EnterNet laboratories (ENLs) participated in the study. Three of the NRLs for Salmonella are also ENLs. The results of these three NRL-ENL laboratories will only be evaluated with the NRLs for Salmonella. In total, 19 strains of the species Salmonella enterica subsp. enterica and one strain of the species Salmonella enterica subsp. arizonae were selected for serotyping and antimicrobial susceptibility testing, while 10 strains of Salmonella Typhimurium (STM) and 10 strains of Salmonella Enteritidis (SE) were selected for phage typing. In general, no problems were encountered with the typing of the O antigens. However, some laboratories had problems with typing the H antigens. Antimicrobial susceptibility testing revealed data showing that standardisation of this technique would be required to allow for comparison between laboratories. The majority of the EnterNet Laboratories and National Reference Laboratories for Salmonella did not encounter major problems with phage typing of STM and SE strains.