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dc.contributor.authorHermanns RCA
dc.contributor.authorZomer G
dc.contributor.authorStavenuiter JFC
dc.contributor.authorWestra G
dc.contributor.authorVisser T
dc.contributor.authorvan de Werken G
dc.date.accessioned2012-12-12T14:46:54Z
dc.date.available2012-12-12T14:46:54Z
dc.date.issued1993-05-31
dc.identifier511004001
dc.identifier.urihttp://hdl.handle.net/10029/256893
dc.description.abstractIn the project 'Oxidative DNA Damage' the first aim is to develop a method for the quantification of 8-Hydroxy-2'-deoxyguanosine (oh8dG). For the method development standard material is needed. In this report the applied route for the synthesis of oh8dG is described. 8-Hydroxy-2'-deoxyguanosine was synthesised starting from 2'-deoxyguanosine (dG) in three steps. In the first step dG was brominated at the C-8 position, resulting in 8-bromo-2'-deoxyguanosine (8-Br-dG). 8-Br-dG was converted into 8-benzyloxy-2'-deoxyguanosine (8-benzyloxy-dG) by treatment of 8-Br-dG with the sodium salt of benzylalcohol. Finally 8-benzyloxy-dG was hydrogenated resulting in 8-hydroxy-2'-deoxyguanosine (oh8dG). Compared to dG, the overall yield of the total procedure was approximately 4%. The total amount produced is 5 mg oh8dG with an estimated purity of 93%, based on HPLC analysis.<br>
dc.description.sponsorshipNKI
dc.description.sponsorshipRIVM
dc.format.extent22 p
dc.language.isoen
dc.publisherRijksinstituut voor Volksgezondheid en Milieu RIVM
dc.relation.ispartofRIVM Rapport 511004001
dc.relation.urlhttp://www.rivm.nl/bibliotheek/rapporten/511004001.html
dc.subject07nl
dc.subjectdna schadenl
dc.subjectoxydatienl
dc.subjectvrije radicalennl
dc.subjectanalysenl
dc.subjectreferentiematerialennl
dc.subjectdna damageen
dc.subjectoxidationen
dc.subjectfree radicalsen
dc.subjectreactive oxygen speciesen
dc.subjectanalysisen
dc.subjectreference materialsen
dc.titleAnalysis of Oxidative DNA Damage, Part I: Synthesis of the standard material 8-hydroxy-2&apos;-deoxyguanosineen
dc.title.alternative[Synthese van 8-hydroxy-2&apos;-deoxyguanosine.]nl
dc.typeReport
dc.date.updated2012-12-12T14:46:55Z
html.description.abstractIn the project &apos;Oxidative DNA Damage&apos; the first aim is to develop a method for the quantification of 8-Hydroxy-2&apos;-deoxyguanosine (oh8dG). For the method development standard material is needed. In this report the applied route for the synthesis of oh8dG is described. 8-Hydroxy-2&apos;-deoxyguanosine was synthesised starting from 2&apos;-deoxyguanosine (dG) in three steps. In the first step dG was brominated at the C-8 position, resulting in 8-bromo-2&apos;-deoxyguanosine (8-Br-dG). 8-Br-dG was converted into 8-benzyloxy-2&apos;-deoxyguanosine (8-benzyloxy-dG) by treatment of 8-Br-dG with the sodium salt of benzylalcohol. Finally 8-benzyloxy-dG was hydrogenated resulting in 8-hydroxy-2&apos;-deoxyguanosine (oh8dG). Compared to dG, the overall yield of the total procedure was approximately 4%. The total amount produced is 5 mg oh8dG with an estimated purity of 93%, based on HPLC analysis.&lt;br&gt;


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