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dc.contributor.authorde Fluiter P
dc.contributor.authorJansen EHJM
dc.date.accessioned2014-01-17T13:29:54
dc.date.issued1992-06-30
dc.identifier618902005
dc.description.abstractA derivatization procedure is described for the long chain fatty acid, lauric acid and metabolites using a fluorescent probe 4-(bromomethyl)-7-methoxycoumarin. The derivatives can be separated and detected in an isocratic high performance liquid chromatografic system using a fluorescence detector. The derivatization is rapid, simple and gives a good quantification by the use of an internal standard. The procedure has been applied on samples from a toxicity experiment with the plasticizer di (2-ethylhexyl)phthalate (DEHP). The activity of cytochrome P-450 IVA1 or lauric acid hydroxylase (LAH) in liver homogenates can be determined by the quantification of the 11- and 12-hydroxylated metabolites of lauric acid. The method turned out to be very sensitive and suitable to replace similar assays using radioactive compounds.<br>
dc.description.sponsorshipHIGB
dc.format.extent17 p
dc.language.isonl
dc.publisherRijksinstituut voor Volksgezondheid en Milieu RIVM
dc.relation.ispartofRIVM Rapport 618902005
dc.relation.urlhttp://www.rivm.nl/bibliotheek/rapporten/618902005.html
dc.subject18nl
dc.subjectlaurinezuurnl
dc.subjectlevernl
dc.subjectanalysenl
dc.subjecthplcnl
dc.subjectftalatennl
dc.subjectderivatiseringnl
dc.subjectp-450nl
dc.subjectlauric acidsen
dc.subjectliveren
dc.subjectanalysisen
dc.subjecthplcen
dc.subjectphthalatesen
dc.titleDerivatisering en HPLC analyse van laurinezuur-metabolieten in leverhomogenaten van rattennl
dc.title.alternativeDerivatization and HPLC analysis of lauric acid metabolites in liverhomogenates of ratsen
dc.typeReport
dc.date.updated2014-01-17T12:32:25Z
html.description.abstractA derivatization procedure is described for the long chain fatty acid, lauric acid and metabolites using a fluorescent probe 4-(bromomethyl)-7-methoxycoumarin. The derivatives can be separated and detected in an isocratic high performance liquid chromatografic system using a fluorescence detector. The derivatization is rapid, simple and gives a good quantification by the use of an internal standard. The procedure has been applied on samples from a toxicity experiment with the plasticizer di (2-ethylhexyl)phthalate (DEHP). The activity of cytochrome P-450 IVA1 or lauric acid hydroxylase (LAH) in liver homogenates can be determined by the quantification of the 11- and 12-hydroxylated metabolites of lauric acid. The method turned out to be very sensitive and suitable to replace similar assays using radioactive compounds.&lt;br&gt;


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