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    EU Collaborative study VI (2002) on bacteriological detection of Salmonella spp.

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    Authors
    Korver H
    Mooijman KA
    Nagelkerke NJD
    van de Giessen AW
    Henken AM
    Type
    Report
    Language
    en
    
    Metadata
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    Title
    EU Collaborative study VI (2002) on bacteriological detection of Salmonella spp.
    Translated Title
    EU Ringonderzoek VI (2002) betreffende bacteriologische dectectie van Salmonella spp.
    Publiekssamenvatting
    Het Communautair Referentie Laboratorium voor Salmonella (CRL-Salmonella, Bilthoven, Nederland) organiseerde in 2002 een zesde bacteriologisch ringonderzoek. Zeventien Nationale Referentie Laboratoria voor Salmonella (NRLs-Salmonella) namen deel aan deze studie. Referentie materialen in combinatie met of zonder de aanwezigheid van kippenfeces, zowel als natuurlijk besmette feces monsters (bevattende Salmonella Infantis) werden getest door alle laboratoria. De referentie materialen bestonden uit gelatine capsules met verschillende besmettingsniveaus Salmonella Typhimurium (STM), Salmonella Enteritidis (SE) of Salmonella Panama (SPan). Bovendien werd naast de uitvoering van de testen van de laboratoria een vergelijking gemaakt tussen de media zoals beschreven in de nieuwe ISO 6579:2002 [Rappaport Vassiliadis Soya Broth (RVS), Mueller Kauffmann Tetrathionate-novobiocin broth (MKTTn) and Xylose Lysine Deoxycholate agar (XLD)] en de alternatieve media Modified Semi-solid Rappaport Vassiliadis (MSRV) en Briljant Groen Agar (BGA). Significant meer positieve isolaties werden gevonden met capsules welke STM bevatten dan met capsules welke SE bevatten, onafhankelijk van het besmettingsniveau of methode. De totale resultaten van alle verschillende capsules zowel als de resultaten van de natuurlijk besmette monsters lieten betere resultaten zien voor MSRV (met BGA en XLD als uitplaat-medium) in vergelijking met de ISO 6579:2002 methode.<br>
    A sixth bacteriological collaborative study was organised by the Community Reference Laboratory for Salmonella (CRL-Salmonella, Bilthoven, The Netherlands) in 2002. Seventeen National Reference Laboratories for Salmonella (NRLs-Salmonella) participated in the study. Reference materials in combination with or without the presence of chicken faeces, as well as naturally contaminated faecal samples (containing Salmonella Infantis) were tested by all laboratories. The reference materials existed of gelatin capsules containing Salmonella Typhimurium (STM), Salmonella Enteritidis (SE) or Salmonella Panama (SPan) at different contamination levels. In addition to the performance testing of the laboratories a comparison was made between the media described in the amended ISO 6579: 2002 [Rappaport Vassiliadis Soya Broth (RVS), Mueller Kauffmann Tetrathionate-novobiocin broth (MKTTn) and Xylose Lysine Deoxycholate agar (XLD)] and the alternative media Modified Semi-solid Rappaport Vassiliadis (MSRV) and Brilliant Green Agar (BGA). Significantly more positive isolations were obtained from capsules containing STM than from capsules containing SE, irrespective of the contamination level or method. The overall results of all different capsules as well as the results of the naturally contaminated samples revealed better results for MSRV (with BGA and XLD as plating-out media) in comparison with the ISO 6579: 2002 method.<br>
    Publisher
    Rijksinstituut voor Volksgezondheid en Milieu RIVM
    Sponsors
    EC-Legislation Veterinaire et Zootechnique
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