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    Het gebruik van kernspinresonantie (NMR) technieken bij de analyse van urine monsters

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    Authors
    Zomer G
    Jacquemijns M
    Stavenuiter JFC
    Type
    Onderzoeksrapport
    Language
    nl
    
    Metadata
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    Title
    Het gebruik van kernspinresonantie (NMR) technieken bij de analyse van urine monsters
    Translated Title
    [The use of Nuclear Magnetic Resonance (NMR) techniques by the analysis of urine samples.]
    Publiekssamenvatting
    1H NMR Spectroscopy yields within a relatively short time useful information on the presence of endogenous metabolites in rat urine. The very intense water signal can be attenuated by lyophilizing the samples and reconstitution of the sample in deuterated water, followed by a saturation pulse of the residual HOD signal. When lyophilized urine samples are compared with non-lyophilized samples no apparent artefacts are introduced. Homonuclear J-resolved 2D- NMR spectroscopy is very well suited to obtain additional structural information. Using this approach an unknown urinary metabolite was identified as N-methyl-3-carboxamide 4-pyridone.<br>
    Publisher
    Rijksinstituut voor Volksgezondheid en Milieu RIVM
    Sponsors
    RIVM
    URI
    http://hdl.handle.net/10029/257952
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    RIVM official reports

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