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dc.contributor.authorVoogd CE
dc.contributor.authorStel JJ van der
dc.date.accessioned2012-12-12T17:03:21Z
dc.date.available2012-12-12T17:03:21Z
dc.date.issued1993-02-28
dc.identifier659001002
dc.identifier.urihttp://hdl.handle.net/10029/258441
dc.description.abstractAbstract niet beschikbaar
dc.description.abstractThe antimutagenic activity of chlorophyllin and hematin was investigated with the Salmonella typhimurium strains TA98 and TA100 by means of the Ames-test. Benzo(a)pyrene, the food pyrolysate 2-amino-1-methyl-6- phenylimidazo [4,5]pyridine or PhIP and 2- aminoanthracene were used as mutagens in most experiments. These agents exert mutagenic activity only in the presence of mammalian metabolic activation. In all experiments the mutagenic activity of the three chemicals could be abolished by the presence of 2 mg of chlorophyllin or 0,5 mg of hematin per selection plate. Considering the results of experiments in which chlorophyllin and hematin were added at several different times after the addition of the metabolic activation system, it was concluded that the formed mutagenic metabolites were trapped and inactivated by these porfyrines. The antimutagenic activities of these porfyrines decreased when the porfyrine ring was destroyed as appeared from experiments with biliverdin and bilirubin. Due to its low solubility the antimutagenic activity of chlorophyll is not or nearly not demonstrable. The antimutagenic activity of chlorophyllin or hematin on substances that are mutagenic in the absence of metabolic activation such as MNNG, furazolidone, dimetridazole, epichlorohydrin and 2-chloroethanol is lower or absent.
dc.description.sponsorshipHIGB
dc.format.extent64 p
dc.language.isonl
dc.relation.ispartofRIVM Rapport 659001002
dc.relation.urlhttp://www.rivm.nl/bibliotheek/rapporten/659001002.html
dc.subject07nl
dc.subjectantimutageniteitstestennl
dc.subjectquercetinnl
dc.subjectmercaptoethanolnl
dc.subjecttestennl
dc.subjectantimutagenic agentsen
dc.subjectquercetinen
dc.subjectmercaptoethanolen
dc.subjecttesting techniquesen
dc.subjectbenzo(a)pyreneen
dc.subject2-aminoanthraceneen
dc.subjectsalmonella typhimuriumen
dc.subjectindol-3-carbinolen
dc.subjectindol-3-carboxaldehydeen
dc.subjectames testen
dc.subjectphip flavonolenen
dc.subjectsulfhydrylverbindingenen
dc.titleDe antimutagene werking van quercetine, kaempferol, myricetine, indol-3-carbinol, indol-3-carboxaldehyde en mercapto-ethanol op de mutagene werking van benzo(a)pyreen, de voedselcontaminant PhIP en 2-aminoanthraceen met Salmonella typhimurium TA98 en TA100nl
dc.title.alternative[The antimutagenic activity of quercetine, kaempferol, myricetin, indol-3-carbinol, indol-3-carboxaldehyde and mercapto-ethanol on the mutagenic activity of benzo(a)pyrene, the food-contaminant PhIP and 2-aminoanthracene with Salmonella typhimurium TA98 and TA100.]en
dc.typeOnderzoeksrapport
dc.date.updated2012-12-12T17:03:21Z
html.description.abstractAbstract niet beschikbaar
html.description.abstractThe antimutagenic activity of chlorophyllin and hematin was investigated with the Salmonella typhimurium strains TA98 and TA100 by means of the Ames-test. Benzo(a)pyrene, the food pyrolysate 2-amino-1-methyl-6- phenylimidazo [4,5]pyridine or PhIP and 2- aminoanthracene were used as mutagens in most experiments. These agents exert mutagenic activity only in the presence of mammalian metabolic activation. In all experiments the mutagenic activity of the three chemicals could be abolished by the presence of 2 mg of chlorophyllin or 0,5 mg of hematin per selection plate. Considering the results of experiments in which chlorophyllin and hematin were added at several different times after the addition of the metabolic activation system, it was concluded that the formed mutagenic metabolites were trapped and inactivated by these porfyrines. The antimutagenic activities of these porfyrines decreased when the porfyrine ring was destroyed as appeared from experiments with biliverdin and bilirubin. Due to its low solubility the antimutagenic activity of chlorophyll is not or nearly not demonstrable. The antimutagenic activity of chlorophyllin or hematin on substances that are mutagenic in the absence of metabolic activation such as MNNG, furazolidone, dimetridazole, epichlorohydrin and 2-chloroethanol is lower or absent.


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