De antimutagene werking van quercetine, kaempferol, myricetine, indol-3-carbinol, indol-3-carboxaldehyde en mercapto-ethanol op de mutagene werking van benzo(a)pyreen, de voedselcontaminant PhIP en 2-aminoanthraceen met Salmonella typhimurium TA98 en TA100
dc.contributor.author | Voogd CE | |
dc.contributor.author | Stel JJ van der | |
dc.date.accessioned | 2012-12-12T17:03:21Z | |
dc.date.available | 2012-12-12T17:03:21Z | |
dc.date.issued | 1993-02-28 | |
dc.identifier | 659001002 | |
dc.identifier.uri | http://hdl.handle.net/10029/258441 | |
dc.description.abstract | Abstract niet beschikbaar | |
dc.description.abstract | The antimutagenic activity of chlorophyllin and hematin was investigated with the Salmonella typhimurium strains TA98 and TA100 by means of the Ames-test. Benzo(a)pyrene, the food pyrolysate 2-amino-1-methyl-6- phenylimidazo [4,5]pyridine or PhIP and 2- aminoanthracene were used as mutagens in most experiments. These agents exert mutagenic activity only in the presence of mammalian metabolic activation. In all experiments the mutagenic activity of the three chemicals could be abolished by the presence of 2 mg of chlorophyllin or 0,5 mg of hematin per selection plate. Considering the results of experiments in which chlorophyllin and hematin were added at several different times after the addition of the metabolic activation system, it was concluded that the formed mutagenic metabolites were trapped and inactivated by these porfyrines. The antimutagenic activities of these porfyrines decreased when the porfyrine ring was destroyed as appeared from experiments with biliverdin and bilirubin. Due to its low solubility the antimutagenic activity of chlorophyll is not or nearly not demonstrable. The antimutagenic activity of chlorophyllin or hematin on substances that are mutagenic in the absence of metabolic activation such as MNNG, furazolidone, dimetridazole, epichlorohydrin and 2-chloroethanol is lower or absent. | |
dc.description.sponsorship | HIGB | |
dc.format.extent | 64 p | |
dc.language.iso | nl | |
dc.relation.ispartof | RIVM Rapport 659001002 | |
dc.relation.url | http://www.rivm.nl/bibliotheek/rapporten/659001002.html | |
dc.subject | 07 | nl |
dc.subject | antimutageniteitstesten | nl |
dc.subject | quercetin | nl |
dc.subject | mercaptoethanol | nl |
dc.subject | testen | nl |
dc.subject | antimutagenic agents | en |
dc.subject | quercetin | en |
dc.subject | mercaptoethanol | en |
dc.subject | testing techniques | en |
dc.subject | benzo(a)pyrene | en |
dc.subject | 2-aminoanthracene | en |
dc.subject | salmonella typhimurium | en |
dc.subject | indol-3-carbinol | en |
dc.subject | indol-3-carboxaldehyde | en |
dc.subject | ames test | en |
dc.subject | phip flavonolen | en |
dc.subject | sulfhydrylverbindingen | en |
dc.title | De antimutagene werking van quercetine, kaempferol, myricetine, indol-3-carbinol, indol-3-carboxaldehyde en mercapto-ethanol op de mutagene werking van benzo(a)pyreen, de voedselcontaminant PhIP en 2-aminoanthraceen met Salmonella typhimurium TA98 en TA100 | nl |
dc.title.alternative | [The antimutagenic activity of quercetine, kaempferol, myricetin, indol-3-carbinol, indol-3-carboxaldehyde and mercapto-ethanol on the mutagenic activity of benzo(a)pyrene, the food-contaminant PhIP and 2-aminoanthracene with Salmonella typhimurium TA98 and TA100.] | en |
dc.type | Report | |
dc.date.updated | 2012-12-12T17:03:21Z | |
html.description.abstract | Abstract niet beschikbaar | |
html.description.abstract | The antimutagenic activity of chlorophyllin and hematin was investigated with the Salmonella typhimurium strains TA98 and TA100 by means of the Ames-test. Benzo(a)pyrene, the food pyrolysate 2-amino-1-methyl-6- phenylimidazo [4,5]pyridine or PhIP and 2- aminoanthracene were used as mutagens in most experiments. These agents exert mutagenic activity only in the presence of mammalian metabolic activation. In all experiments the mutagenic activity of the three chemicals could be abolished by the presence of 2 mg of chlorophyllin or 0,5 mg of hematin per selection plate. Considering the results of experiments in which chlorophyllin and hematin were added at several different times after the addition of the metabolic activation system, it was concluded that the formed mutagenic metabolites were trapped and inactivated by these porfyrines. The antimutagenic activities of these porfyrines decreased when the porfyrine ring was destroyed as appeared from experiments with biliverdin and bilirubin. Due to its low solubility the antimutagenic activity of chlorophyll is not or nearly not demonstrable. The antimutagenic activity of chlorophyllin or hematin on substances that are mutagenic in the absence of metabolic activation such as MNNG, furazolidone, dimetridazole, epichlorohydrin and 2-chloroethanol is lower or absent. |