Ontwikkeling van een toets ter bepaling van subletale effecten van chemische stoffen op regenwormen. V. Vergelijking van twee toetsmethoden ter bepaling van de invloed van bestrijdingsmiddelen op de groei en reproduktie van Eisenia andrei
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TitleOntwikkeling van een toets ter bepaling van subletale effecten van chemische stoffen op regenwormen. V. Vergelijking van twee toetsmethoden ter bepaling van de invloed van bestrijdingsmiddelen op de groei en reproduktie van Eisenia andrei
Translated TitleDevelopment of a test for determining sublethal effects of chemical substances on earthworms. V. Comparison of two test methods for determining the influence of pesticides on growth and reproduction of Eisenia andrei
PubliekssamenvattingThe German Biologische Bundesanstalt (BBA) and RIVM have developed test methods for determining sublethal effects of chemical substances on earthworms. The BBA-method is especially designed for testing pesticides while the RIVM-method is applicable on "all" types of chemicals. After a second ring test, wich showed a considerable variation in results, the BBA method was revised. To compare both methods, the substances parathion and benomyl were tested using the BBA-method and the RIVM-method. In the BBA-method the test substances are sprayed over the soil surface, while they are mixed homogeneously through the soil in the RIVM-method. In order to enable comparison of the results of both test methods, dosages used in the BBA-method were recalculated to concentrations in soil using the equation: kg/ha * 5.3 = mg/kg dry soil. In case of parathion, the BBA-method was more sensitive than the RIVM-method with respect to juveniles/worm/week and number of juveniles/ fertile cocoon. NOEC-values for these parameters, which appeared to be most sensitive in both methods, were about a factor of 10 lower in the BBA method compared to the RIVM-method. It cannot be assessed which method was most sensitive for cocoon production, growth and fertility of the cocoons. In case of benomyl, the RIVM-method was more sensitive than the BBA-method with respect to fertility of the cocoons and the number of juveniles/fertile cocoon. For cocoon production and juveniles/ worm/week the BBA-method was more sensitive. It cannot be assessed which method is most sensitive for growth. For benomyl the final NOEC-values for the most sensitive parameter appeared to be almost similar in both test methods.<br>