• Login
    View Item 
    •   Home
    • RIVM official reports
    • RIVM official reports
    • View Item
    •   Home
    • RIVM official reports
    • RIVM official reports
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of WARPCommunitiesTitleAuthorsIssue DateSubmit DateSubjectsPublisherDepartmentThis CollectionTitleAuthorsIssue DateSubmit DateSubjectsPublisherDepartment

    My Account

    LoginRegister

    Statistics

    Display statistics

    Effects of ultraviolet-B exposure on the resistance to Listeria monocytogenes in the rat

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Average rating
     
       votes
    Cast your vote
    You can rate an item by clicking the amount of stars they wish to award to this item. When enough users have cast their vote on this item, the average rating will also be shown.
    Star rating
     
    Your vote was cast
    Thank you for your feedback
    Authors
    Goettsch W
    Garssen J
    de Klerk A
    Herremans MMPT
    Dortant P
    de Gruijl FR
    van Loveren H
    Type
    Onderzoeksrapport
    Language
    en
    
    Metadata
    Show full item record
    Title
    Effects of ultraviolet-B exposure on the resistance to Listeria monocytogenes in the rat
    Translated Title
    Effecten van UVB blootstelling op de weerstand tegen Listeria monocytogenes in de rat
    Publiekssamenvatting
    Een Listeria monocytogenes infectiemodel in de rat werd gebruikt om de immuunsuppressieve activiteit van ultraviolet-B straling (UVB) te onderzoeken. Ratten werden dagelijks blootgesteld aan suberythemale hoeveelheden UVB straling gedurende 5 of 7 opeenvolgende dagen. Twee verschillende UV bronnen werden gebruikt ; de Kromayer lamp en de FS40 lamp. Na de UVB blootstelling werden de ratten subcutaan of intraveneus geinfecteerd met Listeria. UVB blootstelling leidde tot een toegenomen hoeveelheid bacterien in de milt 4 dagen na infectie. Daarnaast werd met behulp van specifieke lymfocyt- en overgevoeligheidstesten aangetoond dat UVB vooral de cellulaire immuniteit tegen Listeria verminderde 4 en 8 dagen na infectie. Ook werd de fagocyterende activiteit van perifere bloedmacrofagen door UVB straling verminderd. Deze studie gaf aan dat lage hoeveelheden van UVB straling een vertraging in de klaring van Listeria bacterien uit de milt kon veroorzaken. Dit wordt waarschijnlijk veroorzaakt door een verminderde aspecifieke fagocytose van Listeria door macrofagen in combinatie met een verminderde activiteit van de specifieke cellulaire immuunrespons.<br>
    A rat infection model using the bacterial pathogen Listeria monocytogenes was employed to analyze the immunosuppressive activity of ultraviolet-B radiation (UVB). Rats were exposed to suberythemal doses of UVB radiation for 5 or 7 consecutive days, using Kromayer or FS40 lamps respectively. Subsequently, the rats were infected subcutaneously or intravenously with Listeria. UVB exposure resulted in an increased number of bacteria in the spleen 4 days after infection. Listeria-specific lymphocyte proliferation assays as well as DTH reactions demonstrated that T cell-mediated immunity to Listeria was impaired by UVB as measured 4 and 8 days after infection. In addition, UVB exposure decreased phagocytotic activity of peripheral blood macrophages. This study demonstrated that suberythemal doses of UVB radiation caused a delay in the clearance of Listeria bacteria from the spleen of the rats, and that this was probably caused by impaired non-specific phagocytosis of Listeria by macrophages in addition to an impaired activity of Listeria-specific T-cells.<br>
    Publisher
    Rijksinstituut voor Volksgezondheid en Milieu RIVM
    Sponsors
    RIVM
    NOP
    Collections
    RIVM official reports

    entitlement

     

    DSpace software (copyright © 2002 - 2021)  DuraSpace
    Quick Guide | Contact Us
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.