• Login
    View Item 
    •   Home
    • RIVM official reports
    • RIVM official reports
    • View Item
    •   Home
    • RIVM official reports
    • RIVM official reports
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    RIVM Publications RepositoryCommunitiesTitleAuthorsIssue DateSubmit Date

    My Account

    LoginRegister

    Statistics

    Display statistics

    De kippe-embryotest. Fase I. Methodeontwikkeling voor onderzoek aan dioxines en furanan

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Average rating
     
       votes
    Cast your vote
    You can rate an item by clicking the amount of stars they wish to award to this item. When enough users have cast their vote on this item, the average rating will also be shown.
    Star rating
     
    Your vote was cast
    Thank you for your feedback
    Authors
    Kapteijn R
    Vries AD de
    Theelen RMC
    Type
    Report
    Language
    nl
    
    Metadata
    Show full item record
    Title
    De kippe-embryotest. Fase I. Methodeontwikkeling voor onderzoek aan dioxines en furanan
    Translated Title
    [Chicken-embryo test. Phase I. Development of methods to investigate the TEF of dioxines and furans.]
    Publiekssamenvatting
    Abstract niet beschikbaar
    Chicken embryos were used to develop an experimental bioassay to investigate the Toxicological Equivalency Factors (TEF) of dioxines and furans, and mixtures using the TEF principle for risk assessment of dioxins related compunds in reference to the public health. The animals were exposed to the chemicals by a single injection in the air sac of the egg. After incubation, liver microsomes were isolated from the chicken-embryo, in which activity of ethoxyresorufine-o-dealkylase (EROD), and the amount of cytochrome P-450 and protein were determined. The objective of the first phase, reported here, was to optimize the amount of vehicle, the method of isolation, the time of injection and the duration of incubation and exposure. The best suitable vehicle appeared to be 25 mul dioxane, mixed with 25 mul of an isotonic salt solution per egg. Isolation of microsomes by ultracentrifugation turned out to be the mostly effective isolated method, compared to gelfiltration. Injection at the 17th day of incubation and an exposure time of 24 hours, prior to dissection, resulted in the highest EROD induction. The ED50 for EROD induction by 2, 3, 7, 8- tetrachlorodibenzodioxin determined was 10.8 ng/egg. In a second experiment the result was 9.2 ng/egg. For 2,3,4,7,8-pentachlorodibenzofuran the ED50 was assigned to 8.7 ng/egg.
    Sponsors
    HIGB VVP VHI DGM/SVS RIVM
    URI
    http://hdl.handle.net/10029/259214
    Collections
    RIVM official reports

    entitlement

     

    DSpace software (copyright © 2002 - 2023)  DuraSpace
    Quick Guide | Contact Us
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.