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dc.contributor.authorKapteijn R
dc.contributor.authorVries AD de
dc.contributor.authorTheelen RMC
dc.date.accessioned2012-12-12T18:17:57Z
dc.date.available2012-12-12T18:17:57Z
dc.date.issued1993-06-30
dc.identifier618802004
dc.identifier.urihttp://hdl.handle.net/10029/259214
dc.description.abstractAbstract niet beschikbaar
dc.description.abstractChicken embryos were used to develop an experimental bioassay to investigate the Toxicological Equivalency Factors (TEF) of dioxines and furans, and mixtures using the TEF principle for risk assessment of dioxins related compunds in reference to the public health. The animals were exposed to the chemicals by a single injection in the air sac of the egg. After incubation, liver microsomes were isolated from the chicken-embryo, in which activity of ethoxyresorufine-o-dealkylase (EROD), and the amount of cytochrome P-450 and protein were determined. The objective of the first phase, reported here, was to optimize the amount of vehicle, the method of isolation, the time of injection and the duration of incubation and exposure. The best suitable vehicle appeared to be 25 mul dioxane, mixed with 25 mul of an isotonic salt solution per egg. Isolation of microsomes by ultracentrifugation turned out to be the mostly effective isolated method, compared to gelfiltration. Injection at the 17th day of incubation and an exposure time of 24 hours, prior to dissection, resulted in the highest EROD induction. The ED50 for EROD induction by 2, 3, 7, 8- tetrachlorodibenzodioxin determined was 10.8 ng/egg. In a second experiment the result was 9.2 ng/egg. For 2,3,4,7,8-pentachlorodibenzofuran the ED50 was assigned to 8.7 ng/egg.
dc.description.sponsorshipHIGB VVP VHI DGM/SVS RIVM
dc.format.extent29 p
dc.language.isonl
dc.relation.ispartofRIVM Rapport 618802004
dc.relation.urlhttp://www.rivm.nl/bibliotheek/rapporten/618802004.html
dc.subject07nl
dc.subjectdioxinennl
dc.subjectfuranennl
dc.subjectbioassaynl
dc.subjectkippe-embryonl
dc.subjectpcdden
dc.subjectpcdfen
dc.subjectbioassayen
dc.subjectchick-embryoen
dc.subjectdioxinsen
dc.subjectbioassayen
dc.subjectkippe-embryoen
dc.subjectmethode-ontwikkelingen
dc.titleDe kippe-embryotest. Fase I. Methodeontwikkeling voor onderzoek aan dioxines en furanannl
dc.title.alternative[Chicken-embryo test. Phase I. Development of methods to investigate the TEF of dioxines and furans.]en
dc.typeReport
dc.date.updated2012-12-12T18:17:58Z
html.description.abstractAbstract niet beschikbaar
html.description.abstractChicken embryos were used to develop an experimental bioassay to investigate the Toxicological Equivalency Factors (TEF) of dioxines and furans, and mixtures using the TEF principle for risk assessment of dioxins related compunds in reference to the public health. The animals were exposed to the chemicals by a single injection in the air sac of the egg. After incubation, liver microsomes were isolated from the chicken-embryo, in which activity of ethoxyresorufine-o-dealkylase (EROD), and the amount of cytochrome P-450 and protein were determined. The objective of the first phase, reported here, was to optimize the amount of vehicle, the method of isolation, the time of injection and the duration of incubation and exposure. The best suitable vehicle appeared to be 25 mul dioxane, mixed with 25 mul of an isotonic salt solution per egg. Isolation of microsomes by ultracentrifugation turned out to be the mostly effective isolated method, compared to gelfiltration. Injection at the 17th day of incubation and an exposure time of 24 hours, prior to dissection, resulted in the highest EROD induction. The ED50 for EROD induction by 2, 3, 7, 8- tetrachlorodibenzodioxin determined was 10.8 ng/egg. In a second experiment the result was 9.2 ng/egg. For 2,3,4,7,8-pentachlorodibenzofuran the ED50 was assigned to 8.7 ng/egg.


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