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dc.contributor.authorVeld PH in 't
dc.contributor.authorStrijp-Lockefeer NGWM van
dc.contributor.authorHeisterkamp SH
dc.date.accessioned2012-12-12T18:18:27Z
dc.date.available2012-12-12T18:18:27Z
dc.date.issued1993-08-31
dc.identifier281008004
dc.identifier.urihttp://hdl.handle.net/10029/259217
dc.description.abstractAbstract niet beschikbaar
dc.description.abstractA collaborative study was organised to test the newly developed reference materials (RM's) for Bacillus cereus and Staphylococcus aureus. Fourty one laboratories participated in this international collaborative study, each laboratory testing 5 capsules for each of the 2 types of RM. Each series of capsules contained an uncontaminated sample. For use each capsule had to be reconstituted according to a standardized procudure. Both types of RM were enumerated on one non-selective and three selective agars (one of which was chosen by the participating laboratory). The values found for repeatability (r) and reproducibility (R) were ca 1.4 and ca 1.8 respectively independent of the type of medium used. The stability of the RM's was extremely good due to the use of spores in this material. The r and R values were higher for S. aureus than for B. cereus. The r value was 1.6 and the R value, depending on the type of medium used, ranged from 2.0 to 3.4. The stability of this material was poor when stored at 22 degrees C but at -20 degrees C no decrease in contamination level was observed over 70 days of storage.
dc.description.sponsorshipEG/BCR
dc.description.sponsorshipRIVM
dc.format.extent120 p
dc.language.isoen
dc.relation.ispartofRIVM Rapport 281008004
dc.relation.urlhttp://www.rivm.nl/bibliotheek/rapporten/281008004.html
dc.subject11nl
dc.subjectbacillus cereusnl
dc.subjectstaphylococcus aureusnl
dc.subjectvoedingnl
dc.subjectdetectienl
dc.subjectreferentiematerialennl
dc.subjectbacillus cereusen
dc.subjectstaphylococcus aureusen
dc.subjectfooden
dc.subjectidentificationen
dc.subjectreference materialsen
dc.titleEnumeration of Bacillus cereus and Staphylococcus aureus in reference materials for food microbiology. BCR Food/trial 5en
dc.title.alternative[Bepaling van het aantal Bacillus cereus en Staphylococcus aureus kiemen in referentie materialen voor de levensmiddelenmicrobiologie. BCR Food/trial 5.]nl
dc.typeReport
dc.date.updated2012-12-12T18:18:28Z
html.description.abstractAbstract niet beschikbaar
html.description.abstractA collaborative study was organised to test the newly developed reference materials (RM's) for Bacillus cereus and Staphylococcus aureus. Fourty one laboratories participated in this international collaborative study, each laboratory testing 5 capsules for each of the 2 types of RM. Each series of capsules contained an uncontaminated sample. For use each capsule had to be reconstituted according to a standardized procudure. Both types of RM were enumerated on one non-selective and three selective agars (one of which was chosen by the participating laboratory). The values found for repeatability (r) and reproducibility (R) were ca 1.4 and ca 1.8 respectively independent of the type of medium used. The stability of the RM's was extremely good due to the use of spores in this material. The r and R values were higher for S. aureus than for B. cereus. The r value was 1.6 and the R value, depending on the type of medium used, ranged from 2.0 to 3.4. The stability of this material was poor when stored at 22 degrees C but at -20 degrees C no decrease in contamination level was observed over 70 days of storage.


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