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dc.contributor.authorVeld PH in 't
dc.contributor.authorNotermans SHW
dc.date.accessioned2012-12-12T18:34:52Z
dc.date.available2012-12-12T18:34:52Z
dc.date.issued1992-05-31
dc.identifier281008001
dc.identifier.urihttp://hdl.handle.net/10029/259429
dc.description.abstractAbstract niet beschikbaar
dc.description.abstractA study was carried out to test the effect of natural food samples on the isolation of L.monocytogenes from microbiological reference materials. Each of the 30 participating laboratories were given a free choice of enrichment method(s) for the detection of L.monocytogenes, the type(s) of food samples and the number of samples tested. To test the effect of inoculum size reference materials with two levels of contamination (ca 5 and ca 100 cfu/capsule) were provided. Listeria monocytogenes was isolated from 87.8 and 97.8% of the capsules containing 5 and 100 cfu respectively when examined without the addition of food. In the presence of foods the proportions of samples yielding the organism were 63.4% and 72.5% respectively. There was no relation between the isolation methods used or the type of food tested. The results, however, were laboratory dependent. This confirms the need for the performance of individual laboratories to be tested with reference materials both with and without the addition of food samples.
dc.description.sponsorshipEG/BCR HIGB VHI
dc.format.extent29 p
dc.language.isoen
dc.relation.ispartofRIVM Rapport 281008001
dc.relation.urlhttp://www.rivm.nl/bibliotheek/rapporten/281008001.html
dc.subject11nl
dc.subjectlisteria monocytogenesnl
dc.subjectvoedingsmiddelennl
dc.subjectdetectienl
dc.subjectreferentiematerialennl
dc.subjectringonderzoeknl
dc.subjectlisteria monocytogenesen
dc.subjectfooden
dc.subjectidentificationen
dc.subjectreference materialsen
dc.titleDetection of Listeria monocytogenes in foods spiked with reference materials containing Listeria monocytogenesen
dc.title.alternativeDetectie van Listeria monocytogenes in levensmiddelen, kunstmatig besmet met Listeria monocytogenes referentie materialennl
dc.typeReport
dc.date.updated2012-12-12T18:34:53Z
html.description.abstractAbstract niet beschikbaar
html.description.abstractA study was carried out to test the effect of natural food samples on the isolation of L.monocytogenes from microbiological reference materials. Each of the 30 participating laboratories were given a free choice of enrichment method(s) for the detection of L.monocytogenes, the type(s) of food samples and the number of samples tested. To test the effect of inoculum size reference materials with two levels of contamination (ca 5 and ca 100 cfu/capsule) were provided. Listeria monocytogenes was isolated from 87.8 and 97.8% of the capsules containing 5 and 100 cfu respectively when examined without the addition of food. In the presence of foods the proportions of samples yielding the organism were 63.4% and 72.5% respectively. There was no relation between the isolation methods used or the type of food tested. The results, however, were laboratory dependent. This confirms the need for the performance of individual laboratories to be tested with reference materials both with and without the addition of food samples.


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