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    Preparation and characterization of lyophilized antisera against trenbolone and zeranol

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    Authors
    Jansen EHJM
    van den Berg RH
    Enkaalr-Willemsen C
    Zomer G
    Stephany RW
    Type
    Report
    Language
    en
    
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    Title
    Preparation and characterization of lyophilized antisera against trenbolone and zeranol
    Translated Title
    Bereiding en karakterisering van drooggevroren antisera tegen trenbolon en zeranol
    Publiekssamenvatting
    Antisera tegen trenbolon (TB) en zeranol (Z) werden opgewekt in konijnen door immunisatie met het 7-carboxymethyloxime-runder serum albumine (BSA) conjugaat van Z (Z-7CMO-BSA) en het 17-hemisuccinaat-BSA conjugaat van TB (TB-17HS-BSA). De antisera zijn in de geschikte hoeveelheden geampulleerd, drooggevroren en opnieuw gekarakteriseerd. De specificiteit van alle antisera is goed. Zoals verwacht mag worden vertonen de antisera slechts met enkele aan TB of Z nauwverwante stoffen kruisreactiviteit zoals bepaald met radio immunoassay (RIA). Hierdoor zijn de in dit rapport beschreven antisera uitstekend geschikt voor metabolisme studies en voor screeningsdoeleinden. De Z-antisera zijn ook geschikt bevonden in een chemiluminescentie immunoassay (CLIA).<br>
    Publisher
    Rijksinstituut voor Volksgezondheid en Milieu RIVM
    Sponsors
    VHI;
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