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dc.contributor.authorMeiring HD
dc.contributor.authorde Jong APJM
dc.date.accessioned2017-02-20T07:49:22
dc.date.issued1990-02-28
dc.identifier358804001
dc.description.abstractThis report describes the development of a new method for the analysis of the tuberculostaticum isoniazid in human serum. The Laboratory of Chemotherapy needed a sensitive method for the analysis of this compound, which is also useful for complex matrices (e.g. sera of nephritic patients). The developed method is based on a one-step derivatization of isoniazid with pentafluorobenzoylchloride in buffered alkaline aqueous samples. First, reaction takes place on the azid moiety, followed by an intermolecular rearrangement leading to a stable, non-polar derivative, which is easily extracted (>95%) and has excellent gaschromatographic properties. The reaction is rapic (<20 min) and almost pH-independant above pH=7. Highest yields (> 95%) were obtained with strong buffercapacities (1-3 molar) of the sample. For serumsamples, prior proteinprecipitation was necessary in order to obtain similar recoveries as for aqueous standards. The derivative was highly sensitive for negative-ion-chemical-ionization. The detection limit for serum was better than 0.1 mug/ml with sample sizes of 0.5 ml or less.<br>
dc.description.sponsorshipRIVM
dc.format.extent27 p
dc.language.isonl
dc.publisherRijksinstituut voor Volksgezondheid en Milieu RIVM
dc.relation.ispartofRIVM Rapport 358804001
dc.relation.urlhttp://www.rivm.nl/bibliotheek/rapporten/358804001.html
dc.subject18nl
dc.subjecttuberculostaticumnl
dc.subjectgc/nci-msnl
dc.titleOntwikkeling van een verbeterde methode voor de chemische analyse van isoniazide in serum met behulp van pentafluorbenzoyl-derivatisering en gaschromatografie-negatieve chemische ionisatie massaspectrometrienl
dc.title.alternativeDevelopment of an improved method for chemical analysis of isoniazide in serum using pentafluorobenzoyl derivatisation and gaschromatograhphy- negative ion chemical ionization mass spectrometryen
dc.typeReport
dc.date.updated2017-02-20T06:49:23Z
html.description.abstractThis report describes the development of a new method for the analysis of the tuberculostaticum isoniazid in human serum. The Laboratory of Chemotherapy needed a sensitive method for the analysis of this compound, which is also useful for complex matrices (e.g. sera of nephritic patients). The developed method is based on a one-step derivatization of isoniazid with pentafluorobenzoylchloride in buffered alkaline aqueous samples. First, reaction takes place on the azid moiety, followed by an intermolecular rearrangement leading to a stable, non-polar derivative, which is easily extracted (&gt;95%) and has excellent gaschromatographic properties. The reaction is rapic (&lt;20 min) and almost pH-independant above pH=7. Highest yields (&gt; 95%) were obtained with strong buffercapacities (1-3 molar) of the sample. For serumsamples, prior proteinprecipitation was necessary in order to obtain similar recoveries as for aqueous standards. The derivative was highly sensitive for negative-ion-chemical-ionization. The detection limit for serum was better than 0.1 mug/ml with sample sizes of 0.5 ml or less.&lt;br&gt;


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