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dc.contributor.authorStolker AAM
dc.contributor.authorBode W
dc.contributor.authorToet AE
dc.contributor.authorWemer J
dc.contributor.authorGinkel LA van
dc.date.accessioned2012-12-12T18:42:13Z
dc.date.available2012-12-12T18:42:13Z
dc.date.issued1993-06-30
dc.identifier318601001
dc.identifier.urihttp://hdl.handle.net/10029/259547
dc.description.abstractAbstract niet beschikbaar
dc.description.abstractThis report describes a method for the determination of the enantiomers of the beta-blocker propranolol. Propranolol is isolated by solid phase extraction. The propranolol enantiomers are derivatized to diastereomeric compounds by reaction with the chiral reagent (-)-menthylchloroformate. The diastereoisomers are separated by reversed-phase high-performance liquid chromatography (HPLC) on an octadecylcolumn, using methanol-water (875/125 ; v/v) as eluent. The diastereoisomers are detected by fluorescence detection. Standard curves for both R-(+)-propranolol and S-(-)-propranolol are linear in the 0,050-1,50 mg/l range. The limit of detection is 0,010 mg/l for each enantiomer. The limit of determination is 0,050 mg/l for each enantiomer based on a 50 mul plasma. The extraction recoveries are between 95-100%. Within-laboratory reproducibility (n=20) is 5,5% for S-(-)-propranolol and 5,9% for R-(+)-propranolol at 0,352 mg/l. The method has been used for pharmacokinetic studies of propranolol enantiomers in rats.
dc.description.sponsorshipHIG CBG
dc.format.extent27 p
dc.language.isonl
dc.relation.ispartofRIVM Rapport 318601001
dc.relation.urlhttp://www.rivm.nl/bibliotheek/rapporten/318601001.html
dc.subject18nl
dc.subjectpropranololnl
dc.subjectplasmanl
dc.subjectanalysenl
dc.subjecthplcnl
dc.subjectfluorescentienl
dc.subjectpropranololen
dc.subjectplasmaen
dc.subjectanalysisen
dc.subjecthplcen
dc.subjectfluorescenceen
dc.titleDe bepaling van propranolol enantiomeren in ratteplasma met behulp van hoge-druk vloeistofchromatografie en fluorescentie detectie; ontwikkeling en validatienl
dc.title.alternative[The determination of propranolol enantiomers in rat plasma by high-performance liquid chromatography with fluorescence detecion; development and validation.]en
dc.typeReport
dc.date.updated2012-12-12T18:42:14Z
html.description.abstractAbstract niet beschikbaar
html.description.abstractThis report describes a method for the determination of the enantiomers of the beta-blocker propranolol. Propranolol is isolated by solid phase extraction. The propranolol enantiomers are derivatized to diastereomeric compounds by reaction with the chiral reagent (-)-menthylchloroformate. The diastereoisomers are separated by reversed-phase high-performance liquid chromatography (HPLC) on an octadecylcolumn, using methanol-water (875/125 ; v/v) as eluent. The diastereoisomers are detected by fluorescence detection. Standard curves for both R-(+)-propranolol and S-(-)-propranolol are linear in the 0,050-1,50 mg/l range. The limit of detection is 0,010 mg/l for each enantiomer. The limit of determination is 0,050 mg/l for each enantiomer based on a 50 mul plasma. The extraction recoveries are between 95-100%. Within-laboratory reproducibility (n=20) is 5,5% for S-(-)-propranolol and 5,9% for R-(+)-propranolol at 0,352 mg/l. The method has been used for pharmacokinetic studies of propranolol enantiomers in rats.


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