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    Separation and purification of several anabolics present in bovine urine by isocratic high performance liquid chromatography

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    Authors
    Jansen EHJM
    Both-Miedema R
    van Blitterswijk H
    Stephany RW
    Type
    Report
    Language
    en
    
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    Title
    Separation and purification of several anabolics present in bovine urine by isocratic high performance liquid chromatography
    Translated Title
    Zuivering van kalver- en runderurine via hoge druk vloeistofchromatografie voor onderzoek naar de aanwezigheid van verschillende anabolica
    Publiekssamenvatting
    Een eenvoudig isocratisch hoge druk vloeistofchromatografisch systeem wordt beschreven voor de scheiding en zuivering van verschillende typen laag moleculaire anabole verbindingen en enige van hun metabolieten. Voor dit doel worden de verbindingen gechromatografeerd over een "reversed phase" C18 kolom door middel van water-methanol mengsels. Diethylstilbestrol (DES), hexestrol, alfa-R en beta-dienestrol, Zeranol R, zearalenon, zearalanon, 17-beta-Trenbolone R, 19-nortestosteron, 17-alfa-methyltestosteron en medroxyprogesteron worden van elkaar gescheiden. Behalve voor UV-detectie direct gekoppeld aan de vloeistofchromatograaf bleken de geisoleerde fracties ook geschikt voor separaat vervolgonderzoek met (radio)immunochemische detectie, dunnelaagchromatografie, en/of gaschromatografie-massaspectrometrie.
    Publisher
    Rijksinstituut voor Volksgezondheid en Milieu RIVM
    Sponsors
    VHI
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