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    Bereiden en karakteriseren van antisera tegen cytochroom P-450

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    Authors
    Laan CA
    Jansen EHJM
    Type
    Report
    Language
    nl
    
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    Title
    Bereiden en karakteriseren van antisera tegen cytochroom P-450
    Translated Title
    [Preparation and characterization of a number of antibodies against cytochrome P-450.]
    Publiekssamenvatting
    In this report the preparation and characterization of a number of antibodies have been described. These antibodies have been raised in rabbits against cytochrome P-450 isoenzymes of rat liver microsomes. The antigens were isolated from liver microsomes of control rats and rats treated with beta-naftoflavon, phenobarbital and isosarole. Characterization of these antibodies was performed by detection on Western blots. It appeared that antibodies raised against the total isoenzym pattern of cytochrome P-450 showed a good immuno reactivity. A number of antibodies raised against individual cytochrome P-450 isoenzymes also showed an immunological reaction, such as isoenzym IA1 and IA2 from BNF-induced microsomes. The raised antibodies were compared with commercial antibodies against cytochrome P-450, IA1, IIB1, IIE1 and IIIA on Western blots. From this comparison it appeared that besides cytochrome P-450 IA1 also a good antibody has been obtained against cytochrome P-450 IIB1.<br>
    Publisher
    Rijksinstituut voor Volksgezondheid en Milieu RIVM
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