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dc.contributor.authorLaan CA
dc.contributor.authorJansen EHJM
dc.date.accessioned2014-01-17T13:46:27
dc.date.issued1992-11-30
dc.identifier622910003
dc.description.abstractIn this report the preparation and characterization of a number of antibodies have been described. These antibodies have been raised in rabbits against cytochrome P-450 isoenzymes of rat liver microsomes. The antigens were isolated from liver microsomes of control rats and rats treated with beta-naftoflavon, phenobarbital and isosarole. Characterization of these antibodies was performed by detection on Western blots. It appeared that antibodies raised against the total isoenzym pattern of cytochrome P-450 showed a good immuno reactivity. A number of antibodies raised against individual cytochrome P-450 isoenzymes also showed an immunological reaction, such as isoenzym IA1 and IA2 from BNF-induced microsomes. The raised antibodies were compared with commercial antibodies against cytochrome P-450, IA1, IIB1, IIE1 and IIIA on Western blots. From this comparison it appeared that besides cytochrome P-450 IA1 also a good antibody has been obtained against cytochrome P-450 IIB1.<br>
dc.description.sponsorshipRIVM
dc.format.extent35 p
dc.language.isonl
dc.publisherRijksinstituut voor Volksgezondheid en Milieu RIVM
dc.relation.ispartofRIVM Rapport 622910003
dc.relation.urlhttp://www.rivm.nl/bibliotheek/rapporten/622910003.html
dc.subject20nl
dc.subjectcytochroom p-450nl
dc.subjectantiseranl
dc.subjectantilichamennl
dc.subjectantiseraen
dc.subjectantibodiesen
dc.subjectcytochrome p-450en
dc.subjectwestern blottingen
dc.titleBereiden en karakteriseren van antisera tegen cytochroom P-450nl
dc.title.alternative[Preparation and characterization of a number of antibodies against cytochrome P-450.]en
dc.typeReport
dc.date.updated2014-01-17T12:48:57Z
html.description.abstractIn this report the preparation and characterization of a number of antibodies have been described. These antibodies have been raised in rabbits against cytochrome P-450 isoenzymes of rat liver microsomes. The antigens were isolated from liver microsomes of control rats and rats treated with beta-naftoflavon, phenobarbital and isosarole. Characterization of these antibodies was performed by detection on Western blots. It appeared that antibodies raised against the total isoenzym pattern of cytochrome P-450 showed a good immuno reactivity. A number of antibodies raised against individual cytochrome P-450 isoenzymes also showed an immunological reaction, such as isoenzym IA1 and IA2 from BNF-induced microsomes. The raised antibodies were compared with commercial antibodies against cytochrome P-450, IA1, IIB1, IIE1 and IIIA on Western blots. From this comparison it appeared that besides cytochrome P-450 IA1 also a good antibody has been obtained against cytochrome P-450 IIB1.&lt;br&gt;


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