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dc.contributor.authorBisschop A
dc.contributor.authorEigeman L
dc.contributor.authorvan Amsterdam JGC
dc.contributor.authorWemer J
dc.date.accessioned2013-06-13T22:52:15
dc.date.issued1994-01-31
dc.identifier319001004
dc.description.abstractA pilot study was conducted in order to investigate the possibility to measure the IP3 (inositoltrisphosphate) content in cardiovascular tissue of the rat, by means of a radioimmunoassay. With the kit is was possible to obtain a linear standard calibration curve. In extracts of control- and 10 mM NaF stimulated aorta's and left ventricles it appeared that with the applied method the measurement of IP3 was unsuccessful. In contrast to the sample preparation method, the measurement assay is not problematic. It was decided not to continue further investigations.<br>
dc.description.sponsorshipRIVM
dc.format.extent15 p
dc.language.isonl
dc.publisherRijksinstituut voor Volksgezondheid en Milieu RIVM
dc.relation.ispartofRIVM Rapport 319001004
dc.relation.urlhttp://www.rivm.nl/bibliotheek/rapporten/319001004.html
dc.subject03nl
dc.subjectinositol fosfatennl
dc.subjectbepalingnl
dc.subjectradioimmunoassaynl
dc.subjectsecond messenger systeemnl
dc.subjectinositol phosphatesen
dc.subjectassayen
dc.subjectradioimmunoassayen
dc.subjectsecond messenger systemsen
dc.subjectinositoltriphosphateen
dc.titleUitbreiding second messengers: fosfoinositolsysteem. Eindrapportage over pilotexperimentennl
dc.title.alternative[Extension of second messengers: phosphoinositolsystem. Final report on pilotexperiments.]en
dc.typeOnderzoeksrapport
dc.date.updated2013-06-13T20:52:17Z
html.description.abstractA pilot study was conducted in order to investigate the possibility to measure the IP3 (inositoltrisphosphate) content in cardiovascular tissue of the rat, by means of a radioimmunoassay. With the kit is was possible to obtain a linear standard calibration curve. In extracts of control- and 10 mM NaF stimulated aorta&apos;s and left ventricles it appeared that with the applied method the measurement of IP3 was unsuccessful. In contrast to the sample preparation method, the measurement assay is not problematic. It was decided not to continue further investigations.&lt;br&gt;


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