EU Interlaboratory comparison study veterinary XIII (2010) : Detection of Salmonella in chicken faeces
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
Series/Report no.RIVM rapport 330604018
MetadataShow full item record
TitleEU Interlaboratory comparison study veterinary XIII (2010) : Detection of Salmonella in chicken faeces
Translated TitleEU Ringonderzoek veterinair XIII (2010) : Detectie van Salmonella in kippenmest
PubliekssamenvattingIn 2010 waren 31 van de 33 Nationale Referentie Laboratoria (NRL's) in de Europese Unie in staat om hoge en lage concentraties Salmonella in kippenmest aan te tonen. Ze behaalden hiermee een goed resultaat. Twee laboratoria werden beoordeeld met een matig resultaat. Een van deze NRL's had moeite de lage concentraties Salmonella in kippenmest aan te tonen (een gevoeligheidsprobleem). Het andere NRL had problemen het voorgeschreven protocol te volgen, wat deze keer met behulp van een extra controle werd getoetst.
Dit blijkt uit het dertiende veterinair ringonderzoek dat het Communautair Referentie Laboratorium (CRL) voor Salmonella in maart 2010 heeft georganiseerd. Deelname aan dit onderzoek is verplicht voor alle NRL's van de Europese lidstaten die verantwoordelijk zijn voor de detectie van Salmonella. Het CRL-Salmonella is gevestigd bij het Nederlandse Rijksinstituut voor Volksgezondheid en Milieu (RIVM).
Tijdens de studie wordt de internationaal voorgeschreven methode gebruikt om Salmonella aan te tonen in dierlijk mest. Deze methode toonde in meer dan 97 procent van de monsters Salmonella aan.
De laboratoria moesten de studie volgens voorschrift uitvoeren. Elk laboratorium kreeg een pakket toegestuurd met kippenmest (vrij van Salmonella) en 35 gelatine capsules met melkpoeder dat verschillende besmettingsniveaus van Salmonella bevatte. De laboratoria dienden de kippenmest en capsules samen te voegen en vervolgens te onderzoeken of er Salmonella aanwezig was. De extra controle was ingelast om te toetsen of de deelnemende laboratoria daadwerkelijk de kippenmest hadden toegevoegd aan de capsules, wat het protocol voorschrijft. Hiervoor werd een partij kippenmest gemengd met een antibioticum waarvoor de Salmonella die in dit ringonderzoek werden gebruikt, gevoelig zijn. Met als resultaat dat in deze monsters de Salmonella juist niet moest worden aangetroffen.
Thirty-three National Reference Laboratories (NRLs) for Salmonella in the European Union participated in an interlaboratory comparison study in 2010. Of these, thirty-one were able to detect both high and low levels of Salmonella in chicken faeces, thereby achieving the desired proficiency level of 'good performance' for the prescribed method. Two laboratories achieved an assessment of only 'moderate performance'. One of these NRLs had difficulty in detecting low levels of Salmonella (a sensitivity problem). The second experienced problems in following the prescribed protocol which, in this particular study, included an extra control measure.
These are the results of the thirteenth veterinary interlaboratory comparison study organized by the Community Reference Laboratory (CRL) for Salmonella. The study was conducted in March 2010. All NRLs responsible for Salmonella detection from all European Member States were required to participate in this study. The CRL for Salmonella is part of the Dutch National Institute for Public Health and the Environment (RIVM).
The internationally prescribed method for demonstrating the presence of Salmonella in veterinary samples was used during the study. The application thereof resulted in more than 97 percent of the samples being found to be positive for Salmonella.
The laboratories were obligated to conduct the study according to the instructions given. Each laboratory received a package containing chicken faeces (free of Salmonella) and thirty-five gelatine capsules containing powdered milk infected with different levels of Salmonella. The laboratories were instructed to spike samples of chicken faeces with each of the capsules and then test all samples for the presence of Salmonella. The extra control measure was included to check whether all participating laboratories added the faeces to the capsules, which was expressly prescribed in the protocol. It consisted of one batch of chicken faeces mixed with an antibiotic to which the Salmonella used in this ring trial is susceptible. A negative test result for the presence of Salmonella must therefore be obtained in these samples.
Health and Consumer Protection Directorate-General
Showing items related by title, author, creator and subject.
Thirteenth CRL-Salmonella interlaboratory comparison study (2008) on typing of Salmonella spp. : Dertiende CRL-Salmonella ringonderzoek (2008) voor de typering van Salmonella spp.Berk PA; Maas HME; de Pinna E; Mooijman KA; LZO; cib (Rijksinstituut voor Volksgezondheid en Milieu RIVMHealth Protection Agency HPA, 2010-11-04)The National Reference Laboratories (NRLs) of all 27 European Member States performed well on the 2008 quality control test on Salmonella typing. The 4 laboratories which repeated the test also obtained good scores. An analysis of the pooled results from all NRLs revealed that the NRLs taken as a whole were able to assign the correct name to 97 % of the strains tested. One NRL performed the test at a relatively late date and, consequently, its data could not be included in the group analysis. Since 1992, the NRLs have been required to participate in an annual quality control test, which consists of an interlaboratory comparison study for Salmonella typing. Each Member State designates a specific laboratory within their national boundaries to be responsible for the detection and identification of Salmonella strains from samples isolated from animals and/or food products. These laboratories are then referred to as the National Reference Laboratories. The performance of the NRLs is assessed annually based on their capability to correctly identify 20 Salmonella strains. NRLs from countries outside the European Union occasionally participate in these tests, and NRLs from 2 countries belonging to the European Free Trade Association (EFTA) took part in the 2008 test. The expertise of a number of NRLs was subjected to more severe testing by having not only to identify the 20 Salmonella strains of the quality control test but also to subtype (phage typing) various other Salmonella strains. As such, these laboratories received 10 strains of each of Salmonella Enteritidis and Salmonella Typhimurium. These NRLs typed 97 % of the S. Typhimurium strains correctly. The typing of S. Enteritidis strains proved to be more troublesome, with the NRLs typing 94 % of the strains correctly. The Community Reference Laboratory for Salmonella (CRL-Salmonella) organises this annual interlaboratory comparison study in cooperation with the Health Protection Agency in London, UK. The CRL-Salmonella is situated at the National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands.
Sixteenth EURL-Salmonella interlaboratory comparison study (2011) on typing of Salmonella spp. : Zestiende EURL-Salmonella ringonderzoek (2011) voor de typering van Salmonella spp.Jacobs-Reitsma WF; Pol-Hofstad IE; Maas HME; de Pinna E; Mooijman KA; LZO; cib (Rijksinstituut voor Volksgezondheid en Milieu RIVM, 2012-10-24)The 28 National Reference Laboratories (NRLs) of all 27 European Union (EU) Member States performed well on the 2011 quality control test on Salmonella typing. Two laboratories were found to require a follow-up study on their first test. Altogether, the EU-NRLs were able to assign the correct name to 97% of the strains tested. Other participants interlaboratory comparison study Salmonella Since 1992, the NRLs of the EU Member States have been required to participate in annual quality control tests, which consist of interlaboratory comparison studies on Salmonella. Laboratories from countries outside the European Union, like EU-candidate countries, occasionally participate in these tests on a voluntary basis. Eight additional laboratories participated in the current study. Two EUcandidate countries amongst these eight additional participants did not meet the criteria for good performance in the first round. One of them did not reach this goal in the follow-up study either. The other was not able to participate in the follow-up study; a follow-up study is not compulsory for non-EU laboratories. Each EU Member State designates a specific laboratory within their national boundaries to be responsible for the detection and identification of Salmonella strains from animals and/or food products. These laboratories are then referred to as the National Reference Laboratories. The performance of these NRLs on Salmonella typing is assessed annually, based on their capability to correctly identify 20 Salmonella strains. Phage typing Nine NRLs not only serotyped the 20 Salmonella strains of the quality control test, but also subtyped 20 additional strains by phage typing. For this, the laboratories received ten strains of Salmonella Enteritidis and ten strains of Salmonella Typhimurium. These NRLs typed 98% of the S. Typhimurium strains correctly. Of the S. Enteritidis strains, 88% were phage typed correctly. The European Union Reference Laboratory for Salmonella (EURL-Salmonella) organises this annual interlaboratory comparison study on typing of Salmonella in cooperation with the Health Protection Agency in London, UK. The EURLSalmonella is situated at the National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands.
Test results of Salmonella typing by the NRLs-Salmonella in the Member States of the EU and the EnterNet Laboratories - Collaborative study VI (2001) on typing of SalmonellaKorver H; Raes M; Maas HME; Ward LR; Wannet WJB; Henken AM; MGB; LIS (PHLS-Colindale/London, 2002-09-06)Test results of Salmonella sero- and phage typing and antimicrobial susceptibility testing by the National Reference Laboratories for Salmonella in the Member States of the European Union and the EnterNet Laboratories: Collaborative study VI (2001) for Salmonella. The sixth collaborative typing study for Salmonella was organised by the Community Reference Laboratory for Salmonella (CRL-Salmonella, Bilthoven, The Netherlands) in collaboration with the Public Health Laboratory Services (PHLS), London, UK. Seventeen National Reference Laboratories for Salmonella (NRLs-Salmonella) and 15 EnterNet laboratories (ENLs) participated in the study. Three of the NRLs for Salmonella are also ENLs. The results of these three NRL-ENL laboratories will only be evaluated with the NRLs for Salmonella. In total, 19 strains of the species Salmonella enterica subsp. enterica and one strain of the species Salmonella enterica subsp. arizonae were selected for serotyping and antimicrobial susceptibility testing, while 10 strains of Salmonella Typhimurium (STM) and 10 strains of Salmonella Enteritidis (SE) were selected for phage typing. In general, no problems were encountered with the typing of the O antigens. However, some laboratories had problems with typing the H antigens. Antimicrobial susceptibility testing revealed data showing that standardisation of this technique would be required to allow for comparison between laboratories. The majority of the EnterNet Laboratories and National Reference Laboratories for Salmonella did not encounter major problems with phage typing of STM and SE strains.