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    Determination of histamine and 1-methylhistamine in human plasma by high-performance liquid chromatography and fluorescence detection

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    Authors
    Wubs KL
    Stolker AAM
    Kortboyer JM
    Meulenbelt J
    van Ginkel LA
    Type
    Report
    Language
    en
    
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    Title
    Determination of histamine and 1-methylhistamine in human plasma by high-performance liquid chromatography and fluorescence detection
    Translated Title
    Bepaling van histamine en 1-methylhistamine in humaan plasma met behulp van hoge-druk vloeistofchromatografie en fluorescentie detectie
    Publiekssamenvatting
    Voor de bepaling van histamine en 1-methylhistamine (=Nt-methylhistamine=histamine metaboliet) in humaanplasma is de vloeistofchromatografische methode gebruikt. De uit het plasma geextraheerde histaminen werden met behulp van fluorescamine gederivatiseerd tot fluorescerende verbindingen. De derivaten werden met behulp van HPLC gescheiden op een octadecylsilica-kolom met als eluens een mengsel van 25 mM fosfaatbuffer (pH=9.1)/ acetronitril. De derivaten werden met behulp van een fluorescentiedetector gedetecteerd. De binnen laboratorium reproduceerbaarheid varieerde voor histamine tussen 52-7% (n=2) binnen het concentratiegebied van 1.7-24.7 mug/l en dat voor het 1-methylhistamine varieerde van 86-41% (n=2) binnen het concentratiegebied van 1.4-20.4 mug/l. Deze waarden waren voor histamine acceptabel maar te hoog voor het kwantitatief bepalen van 1-methylhistamine.<br>
    This report describes a method for the determination of histamine and 1-methylhistamine (=Nt-methylhistamine) in samples of human plasma. The analytes were extracted from the sample using a cation-exchange solid-phase extraction cartridge and derivatized to fluorescense compounds with fluorescamine. The derivatives were detected using a fluorescence detection. The standard curve for histamine was linear within the range of 1.7-24.7 mug/l and for 1-methylhistamine within the range of 1.4-20.4 mug/l. The within-laboratory reproducibility for histamine varied from 52-7% (n=2) for the concentration range of 1.7-24.7 mug/l. The within-laboratory reproducibility for 1-methylhistamine varied from 86-41% (n=2) for the concentration range of 1.4-20.4 mug/l. These values were acceptable for histamine but too high for accurate quantification of 1-methylhistamine.<br>
    Publisher
    Rijksinstituut voor Volksgezondheid en Milieu RIVM
    Sponsors
    IGB
    URI
    http://hdl.handle.net/10029/261145
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