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dc.contributor.authorBosveld ATC
dc.contributor.authorTheelen RMC
dc.date.accessioned2012-12-12T22:18:06Z
dc.date.available2012-12-12T22:18:06Z
dc.date.issued1993-06-30
dc.identifier618802005
dc.identifier.urihttp://hdl.handle.net/10029/261412
dc.description.abstractAbstract niet beschikbaar
dc.description.abstractIn order to evaluate the risk of samples contaminated with mixtures polychlorinated dibenzo-p-dioxins, polychlorinated dibenzofurans and planar biphenyls a chicken embryo bioassay is in development at our Institute. In this bioassay the toxic potency of these compounds is determined by means of the ethoxyresorufin-deethylation (EROD) activity in the microsomal fraction of the chicken embryo liver. The experiments showed a large variation between the basal EROD activities, and the maximal induced EROD activities. The slope for most dose curves was similar. The relative toxicity factors in the chick embryo test determined, were compared to the international (i)-TEFs. The EROD induction by the tetra- and penta-dioxins and -furans showed higher TEF in the chicken embryo compared to the (i)-TEF, and the hexa- and heptachlorinated congeners are undervalued in the chicken embryo assay. Then the chicken embryo assay was used to evaluate the additive aspect of the TEF principle. The results showed that additivity of the congeners was responsible for the EROD induction at a low dose.
dc.description.sponsorshipHIGB VVP VHI;DGM/SVS RIVM
dc.format.extent42 p
dc.language.isonl
dc.relation.ispartofRIVM Rapport 618802005
dc.relation.urlhttp://www.rivm.nl/bibliotheek/rapporten/618802005.html
dc.subject07nl
dc.subjectdioxinennl
dc.subjectbioassaynl
dc.subjectkippen-embryonl
dc.subjectpcdden
dc.subjectbioassayen
dc.subjectchicken-embryoen
dc.subjectkippe-embryo testen
dc.subjecttefen
dc.subjectmengselinteractieen
dc.titleDe kippe-embryo test. Fase II. Bepaling van de relatieve toxiciteit van elf 2,3,7,8-chloorgesubstitueerde dioxinen en furanan, en drie planaire PCB'snl
dc.title.alternative[Chicken-embryo test. Fase II. Determination of the relative toxicity of eleven 2,3,7,8-chlorosubstituted dioxines and furanes, and three planary OCB's.]en
dc.typeReport
dc.date.updated2012-12-12T22:18:06Z
html.description.abstractAbstract niet beschikbaar
html.description.abstractIn order to evaluate the risk of samples contaminated with mixtures polychlorinated dibenzo-p-dioxins, polychlorinated dibenzofurans and planar biphenyls a chicken embryo bioassay is in development at our Institute. In this bioassay the toxic potency of these compounds is determined by means of the ethoxyresorufin-deethylation (EROD) activity in the microsomal fraction of the chicken embryo liver. The experiments showed a large variation between the basal EROD activities, and the maximal induced EROD activities. The slope for most dose curves was similar. The relative toxicity factors in the chick embryo test determined, were compared to the international (i)-TEFs. The EROD induction by the tetra- and penta-dioxins and -furans showed higher TEF in the chicken embryo compared to the (i)-TEF, and the hexa- and heptachlorinated congeners are undervalued in the chicken embryo assay. Then the chicken embryo assay was used to evaluate the additive aspect of the TEF principle. The results showed that additivity of the congeners was responsible for the EROD induction at a low dose.


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