• Login
    View Item 
    •   Home
    • RIVM official reports
    • RIVM official reports
    • View Item
    •   Home
    • RIVM official reports
    • RIVM official reports
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    RIVM Publications RepositoryCommunitiesTitleAuthorsIssue DateSubmit Date

    My Account

    LoginRegister

    Statistics

    Display statistics

    Diagnostiek en epidemiologie van rotavirusinfecties in Nederland

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Average rating
     
       votes
    Cast your vote
    You can rate an item by clicking the amount of stars they wish to award to this item. When enough users have cast their vote on this item, the average rating will also be shown.
    Star rating
     
    Your vote was cast
    Thank you for your feedback
    Authors
    Vinje J
    van der Avoort HGAM
    Kaan JA
    van Loon AM
    Type
    Report
    Language
    nl
    
    Metadata
    Show full item record
    Title
    Diagnostiek en epidemiologie van rotavirusinfecties in Nederland
    Translated Title
    Diagnosis and epidemiology of rotavirus infections in the Netherlands
    Publiekssamenvatting
    In the Netherlands the infection occurs predominantly during the first four years of live and the seasonal activity is highest during early spring. The diagnosis is made more frequently in boys than in girls (56% vs 44%). Most virological laboratories in the Netherlands use latex agglutination (LA) tests for the diagnosis of rotavirus. A total of 205 human faecal specimens were examined for the presence of rotavirus by four different detection methods: latex agglutination (LA) test, a "home-made" enzyme immuno-assay (EIA), polyacrylamide gel electrophoresis (PAGE) and agarose gel electrophoresis (AGE). Identical results in all four tests were found in 188 (92%) of the stool specimens. Using the results of a newly defined golden standard: TRUE, we found the specificities of the LA, EIA, PAGE and AGE to be 97.9, 98.6, 100 and 100%, respectively. The sensitivities of these four methods were 98.4, 98.4, 88.7 and 88.7% respectively. Early diagnosis of rotavirus infection is essential for effective patient management and infection control. LA and AGE are both rapid screening tests for the diagnosis of rotavirus infection. AGE is also an inexpensive test with an added advantage that this method can detect non-group A rotaviruses. During our survey, we could not identify non-group A rotaviruses. The epidemiology of rotavirus gastroenteritis in the Netherlands was investigated by serotyping. We compared two different methods for serotyping 84 faecal rotavirusstrains from patients with gastroenteritis: serotyping by an enzyme immunoassay with serotype-specific monoclonal antibodies and gen amplification with type-specific oligonucleotide primers. With the PCR typing method we could type 96%, and with the EIA MAb 82% of the 84 faecal strains. 100% agreement of the result was observed with all the samples that were typed by both assays. Serotype 1 rotavirus was predominant in the Netherlands in 1990 with a prevalence of 77%. The prevalence of serotype 2,3 and 4 was 3,3 and 17% respectively.<br>
    Publisher
    Rijksinstituut voor Volksgezondheid en Milieu RIVM
    Sponsors
    GHI
    Collections
    RIVM official reports

    entitlement

     

    DSpace software (copyright © 2002 - 2023)  DuraSpace
    Quick Guide | Contact Us
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.