• Login
    View Item 
    •   Home
    • RIVM official reports
    • RIVM official reports
    • View Item
    •   Home
    • RIVM official reports
    • RIVM official reports
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    RIVM Publications RepositoryCommunitiesTitleAuthorsIssue DateSubmit Date

    My Account

    LoginRegister

    Statistics

    Display statistics

    Survival of E.coli K12 on laboratory coats made of 100% cotton

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Average rating
     
       votes
    Cast your vote
    You can rate an item by clicking the amount of stars they wish to award to this item. When enough users have cast their vote on this item, the average rating will also be shown.
    Star rating
     
    Your vote was cast
    Thank you for your feedback
    Authors
    Canter Cremers HCJ
    Groot HF
    Type
    Report
    Language
    en
    
    Metadata
    Show full item record
    Title
    Survival of E.coli K12 on laboratory coats made of 100% cotton
    Translated Title
    Overleving van E.coli K12 op katoenen laboratorium jassen
    Publiekssamenvatting
    Abstract niet beschikbaar
    The research presented in this report focusses on the survival of E.coli K12 bacteria on laboratory coats made of 100% cotton. Therefore aliquots of cultures were applied to small pieces cut from a lab coat, which were then dried in a safety cabinet. Viable E.coli K12 bacteria could be reisolated from such a piece of cloth with high efficiency by incubating it for 5 minutes in either saline or tap water at 35 degr. C. Optimal results were however obtained by incubation for 90 minutes in saline at room temperatures, which became the standard reisolation procedure. In this way, viable bacteria of E.coli K12 strain KMBL1164 could be reisolated till at least twenty days after application and drying. Survival of strain KMBL1164 was not influenced by the number of viable bacteria present in the culture or the presence of additional DNA in the form of plasmid pMP92, which is 7.2 kb in size. Survival was however influenced by the quantity of culture applied, the culturing phase at the moment of application and the chromosomal background of the bacteria applied. Furthermore E.coli K12 strain KMBL1164 survived drying on a piece of a lab coat about as well as a wild type E.coli strain isolated from faeces or E.coli type B and C strains. The first step in the commercial washing procedure of lab coats can consist of rinsing them in water at 35 degr. C. This water is then discharged into the sewage system. Since they undergo no special treatment before they are washed, our results indicate that (genetically modified-) E.coli K12 bacteria can be released into the sewage system during the washing procedure of lab coats worn by workers in recombinant DNA laboratoris. Several other ways in which genetically modified E.coli K12 strains can be released from the laboratory space are discussed.
    Sponsors
    DGM/SR
    URI
    http://hdl.handle.net/10029/261635
    Collections
    RIVM official reports

    entitlement

     

    DSpace software (copyright © 2002 - 2023)  DuraSpace
    Quick Guide | Contact Us
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.