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dc.contributor.authorHendriksen CFM
dc.contributor.authorWoltjes J
dc.contributor.authorGun JW van de
dc.contributor.authorAkkermans AM
dc.contributor.authorMarsman JW
dc.contributor.authorVerschure MH
dc.contributor.authorVeldman K
dc.date.accessioned2012-12-12T22:53:36Z
dc.date.available2012-12-12T22:53:36Z
dc.date.issued1993-05-31
dc.identifier949106001
dc.identifier.urihttp://hdl.handle.net/10029/261797
dc.description.abstractAbstract niet beschikbaar
dc.description.abstractAccording to the specifications of the European Pharmacopoeia (EP) monograph on Tetanus vaccine for veterinary use (EP 1990) potency testing is based either on immunization and bleeding of guinea-pigs or rabbits, followed by estimation of the antitoxin titre by toxin neutralization assay (TN test) in mice (Method A) or on immunization and challenge of mice (Method B). Recently in vitro serological assay systems have been described as potential alternatives to the TN test. These include Enzyme Linked Immunosorbent Assay (ELISA), Toxin Binding Inhibition (ToBI) test, Haemagglutination (HA) test and modifications of these procedures such as antigen capture ELISA and antigen competition ELISA. An interlaboratory validation study was initiated to evaluate the suitability of the in vitro serological methods to provide valid and reproducible measurements of the biological potency by Method A of the EP. This report presents the results of this study. Intra- and interlaboratory variation was acceptable for ELISA and ToBI test, but large variation was seen in the HA test. Estimates of potency obtained by TN test and by in vitro test systems were in good agreement for a wide range of products and a wide range of antitoxin titres. It is concluded that the TN test for the estimation of potency of tetanus toxoid vaccines for veterinary use according to Method A of the EP can be replaced by ELISA or ToBI test. Standardization of HA test is required before this test can be used for quality control purposes.
dc.description.sponsorshipEG
dc.format.extent41 p
dc.language.isoen
dc.relation.ispartofRIVM Rapport 949106001
dc.relation.urlhttp://www.rivm.nl/bibliotheek/rapporten/949106001.html
dc.subject11nl
dc.subjecttetanus toxoidnl
dc.subjectveterinaire vaccinsnl
dc.subjectkwaliteitscontrolenl
dc.subjectserodiagnostieknl
dc.subjectbepalingnl
dc.subjectelisanl
dc.subjectvalidatienl
dc.subjecttetanus toxoiden
dc.subjectveterinary vaccinesen
dc.subjectquality controlen
dc.subjectserodiagnosisen
dc.subjectassayen
dc.subjectelisaen
dc.subjectvalidationen
dc.subjectpotency testen
dc.subjecttn-testen
dc.subjectha-testen
dc.subjecttobi-test tn testen
dc.subjectelisaen
dc.subjectha-testen
dc.subjecttobi testen
dc.titleInterlaboratory validation of in vitro serological assay systems for potency testing of tetanus toxoid vaccines for veterinary useen
dc.title.alternative[Inter-laboratorium validatie van in vitro serologische modellen ten behoeve van het werkzaamheidsonderzoek van tetanus vaccins voor veterinair gebruik.]nl
dc.typeReport
dc.date.updated2012-12-12T22:53:37Z
html.description.abstractAbstract niet beschikbaar
html.description.abstractAccording to the specifications of the European Pharmacopoeia (EP) monograph on Tetanus vaccine for veterinary use (EP 1990) potency testing is based either on immunization and bleeding of guinea-pigs or rabbits, followed by estimation of the antitoxin titre by toxin neutralization assay (TN test) in mice (Method A) or on immunization and challenge of mice (Method B). Recently in vitro serological assay systems have been described as potential alternatives to the TN test. These include Enzyme Linked Immunosorbent Assay (ELISA), Toxin Binding Inhibition (ToBI) test, Haemagglutination (HA) test and modifications of these procedures such as antigen capture ELISA and antigen competition ELISA. An interlaboratory validation study was initiated to evaluate the suitability of the in vitro serological methods to provide valid and reproducible measurements of the biological potency by Method A of the EP. This report presents the results of this study. Intra- and interlaboratory variation was acceptable for ELISA and ToBI test, but large variation was seen in the HA test. Estimates of potency obtained by TN test and by in vitro test systems were in good agreement for a wide range of products and a wide range of antitoxin titres. It is concluded that the TN test for the estimation of potency of tetanus toxoid vaccines for veterinary use according to Method A of the EP can be replaced by ELISA or ToBI test. Standardization of HA test is required before this test can be used for quality control purposes.


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