• Next Generation HGVS Nomenclature Checker.

      Lefter, Mihai; Vis, Jonathan K; Vermaat, Martijn; den Dunnen, Johan T; Taschner, Peter E M; Laros, Jeroen F J (2021-02-04)
    • The importance of supplementary immunisation activities to prevent measles outbreaks during the COVID-19 pandemic in Kenya.

      Mburu, C N; Ojal, J; Chebet, R; Akech, D; Karia, B; Tuju, J; Sigilai, A; Abbas, K; Jit, M; Funk, S; et al. (2021-02-03)
    • Modelling the impact of tailored behavioural interventions on chlamydia transmission.

      van Wees, Daphne A; den Daas, Chantal; Kretzschmar, Mirjam E E; Heijne, Janneke C M (2021-01-25)
    • Adsorption of titanium dioxide nanoparticles onto zebrafish eggs affects colonizing microbiota.

      Brinkmann, Bregje W; Beijk, Wouter F; Vlieg, Redmar C; van Noort, S John T; Mejia, Jorge; Colaux, Julien L; Lucas, Stéphane; Lamers, Gerda; Peijnenburg, Willie J G M; Vijver, Martina G (2021-01-24)
      Teleost fish embryos are protected by two acellular membranes against particulate pollutants that are present in the water column. These membranes provide an effective barrier preventing particle uptake. In this study, we tested the hypothesis that the adsorption of antimicrobial titanium dioxide nanoparticles onto zebrafish eggs nevertheless harms the developing embryo by disturbing early microbial colonization. Zebrafish eggs were exposed during their first day of development to 2, 5 and 10 mg TiO2 L-1 (NM-105). Additionally, eggs were exposed to gold nanorods to assess the effectiveness of the eggs' membranes in preventing particle uptake, localizing these particles by way of two-photon microscopy. This confirmed that particles accumulate onto zebrafish eggs, without any detectable amounts of particles crossing the protective membranes. By way of particle-induced X-ray emission analysis, we inferred that the titanium dioxide particles could cover 25-45 % of the zebrafish egg surface, where the concentrations of sorbed titanium correlated positively with concentrations of potassium and correlated negatively with concentrations of silicon. A combination of imaging and culture-based microbial identification techniques revealed that the adsorbed particles exerted antimicrobial effects, but resulted in an overall increase of microbial abundance, without any change in heterotrophic microbial activity, as inferred based on carbon substrate utilization. This effect persisted upon hatching, since larvae from particle-exposed eggs still comprised higher microbial abundance than larvae that hatched from control eggs. Notably, pathogenic aeromonads tolerated the antimicrobial properties of the nanoparticles. Overall, our results show that the adsorption of suspended antimicrobial nanoparticles on aquatic eggs can have cascading effects across different life stages of oviparous animals. Our study furthermore suggests that aggregation dynamics may occur that could facilitate the dispersal of pathogenic bacteria through aquatic ecosystems.
    • Unlike dietary restriction, rapamycin fails to extend lifespan and reduce transcription stress in progeroid DNA repair-deficient mice.

      Birkisdóttir, María B; Jaarsma, Dick; Brandt, Renata M C; Barnhoorn, Sander; van Vliet, Nicole; Imholz, Sandra; van Oostrom, Conny T; Nagarajah, Bhawani; Portilla Fernández, Eliana; Roks, Anton J M; et al. (2021-01-23)
      Dietary restriction (DR) and rapamycin extend healthspan and life span across multiple species. We have recently shown that DR in progeroid DNA repair-deficient mice dramatically extended healthspan and trippled life span. Here, we show that rapamycin, while significantly lowering mTOR signaling, failed to improve life span nor healthspan of DNA repair-deficient Ercc1∆/- mice, contrary to DR tested in parallel. Rapamycin interventions focusing on dosage, gender, and timing all were unable to alter life span. Even genetically modifying mTOR signaling failed to increase life span of DNA repair-deficient mice. The absence of effects by rapamycin on P53 in brain and transcription stress in liver is in sharp contrast with results obtained by DR, and appoints reducing DNA damage and transcription stress as an important mode of action of DR, lacking by rapamycin. Together, this indicates that mTOR inhibition does not mediate the beneficial effects of DR in progeroid mice, revealing that DR and rapamycin strongly differ in their modes of action.
    • Development and usability of educational material about workplace particulate matter exposure.

      Stege, T A M; Bolte, J F B; Claassen, L; Timmermans, D R M (2021-01-22)
    • Absence of association between 2019-20 influenza vaccination and COVID-19: Results of the European I-MOVE-COVID-19 primary care project, March-August 2020.

      Kissling, Esther; Hooiveld, Mariëtte; Brytting, Mia; Vilcu, Ana-Maria; de Lange, Marit; Martínez-Baz, Iván; Sigerson, Debbie; Enkirch, Theresa; Belhillil, Sylvie; Meijer, Adam; et al. (2021-01-22)
    • Effect of UV/chlorine treatment on photophysical and photochemical properties of dissolved organic matter.

      Zhou, Yangjian; Cheng, Fangyuan; He, Dongyang; Zhang, Ya-Nan; Qu, Jiao; Yang, Xin; Chen, Jingwen; Peijnenburg, Willie J G M (2021-01-20)
      Dissolved organic matter (DOM) is a ubiquitous component in effluents, DOM discharged with an effluent can affect the composition and properties of natural DOM in the receiving waters. As the photophysical and photochemical properties of effluent DOM can be changed by wastewater treatment processes, the effect of UV/chlorine treatment on the photophysical and photochemical properties of DOM was investigated using Suwannee River fulvic acid (SRFA) and Suwannee River natural organic matter (SRNOM) as representatives. Results showed that the absorbance of the two DOM was significantly decreased. The evolution trends of three representative photophysical parameters upon increase of chlorine dosages were observed. Also, a decrease in DOM aromaticity, molecular weight and electron-donating capacity was observed upon increasing chlorine dosage. Quantum yields of excited triplet state of DOM (3DOM*), singlet oxygen (1O2) and hydroxyl radicals (·OH) first decreases and then increased in the UV/chlorine systems upon increasing chlorine dosages due to the different reaction pathways of the two DOM. Moreover, 3DOM* can not only be regarded as a "controller" of other reactive intermediates, but also effectively promote the photodegradation of bezafibrate, which is classified as a persistent organic contaminant. This study gives deep insights into effects of UV/chlorine on the photophysical and photochemical properties of DOM, and is helpful for understanding the dynamic roles of DOM in the photodegradation of micropollutants.
    • Societal views in the Netherlands on active disinvestment of publicly funded healthcare interventions.

      Rotteveel, A H; Reckers-Droog, V T; Lambooij, M S; de Wit, G A; van Exel, N J A (2021-01-20)
    • Riverine microplastic and microbial community compositions: A field study in the netherlands.

      Mughini-Gras, Lapo; van der Plaats, Rozemarijn Q J; van der Wielen, Paul W J J; Bauerlein, Patrick S; de Roda Husman, Ana Maria (2021-01-19)
      Plastic pollution in aquatic environments, particularly microplastics (<5 mm), is an emerging health threat. The buoyancy, hydrophobic hard surfaces, novel polymer carbon sources and long-distance transport make microplastics a unique substrate for biofilms, potentially harbouring pathogens and enabling antimicrobial resistance (AMR) gene exchange. Microplastic concentrations, their polymer types and the associated microbial communities were determined in paired, contemporaneous samples from the Dutch portion of the river Rhine. Microplastics were collected through a cascade of 500/100/10 μm sieves; filtrates and surface water were also analysed. Microplastics were characterized with infrared spectroscopy. Microbial communities and selected virulence and AMR genes were determined with 16S rRNA-sequencing and qPCR. Average microplastic concentration was 213,147 particles/m3; polyamide and polyvinylchloride were the most abundant polymers. Microbial composition on 100-500 μm samples differed significantly from surface water and 10-100 μm or smaller samples, with lower microbial diversity compared to surface water. An increasingly 'water-like' microbial community was observed as particles became smaller. Associations amongst specific microbial taxa, polymer types and particle sizes, as well as seasonal and methodological effects, were also observed. Known biofilm-forming and plastic-degrading taxa (e.g. Pseudomonas) and taxa harbouring potential pathogens (Pseudomonas, Acinetobacter, Arcobacter) were enriched in certain sample types, and other risk-conferring signatures like the sul1 and erm(B) AMR genes were almost ubiquitous. Results were generally compatible with the existence of taxon-selecting mechanisms and reduced microbial diversity in the biofilms of plastic substrates, varying over seasons, polymer types and particle sizes. This study provided updated field data and insights on microplastic pollution in a major riverine environment.
    • Uptake of fetal aneuploidy screening after the introduction of the non-invasive prenatal test: a national population-based register study.

      van der Meij, Karuna R M; de Groot-van Mooren, Maurike; Carbo, Ellen W S; Pieters, Mijntje J; Rodenburg, Wendy; Sistermans, Erik A; Cornel, Martina C; Henneman, Lidewij (2021-01-19)
    • The occurrence of non-anatomical therapeutic chemical-international nonproprietary name molecules in suspected illegal or illegally traded health products in Europe: A retrospective and prospective study.

      Deconinck, Eric; Vanhee, Celine; Keizers, Peter; Guinot, Pauline; Mihailova, Albena; Syversen, Per Vidar; Li-Ship, Graziella; Young, Steven; Blazewicz, Agata; Poplawska, Magdalena; et al. (2021-01-16)
    • Applications and Recruitment Performance of Web-Based Respondent-Driven Sampling: Scoping Review.

      Helms, Yannick B; Hamdiui, Nora; Kretzschmar, Mirjam E E; Rocha, Luis E C; van Steenbergen, Jim E; Bengtsson, Linus; Thorson, Anna; Timen, Aura; Stein, Mart L (2021-01-15)
    • Exploring determinants of hand hygiene compliance in LTCFs: a qualitative study using Flottorps' integrated checklist of determinants of practice.

      Lescure, Dominique; Haenen, Anja; de Greeff, Sabine; Voss, Andreas; Huis, Anita; Hulscher, Marlies (2021-01-14)
    • Immunogenicity, duration of protection, effectiveness and safety of rubella containing vaccines: A systematic literature review and meta-analysis.

      Van den Boogaard, Jossy; de Gier, Brechje; de Oliveira Bressane Lima, Priscila; Desai, Shalini; de Melker, Hester E; Hahné, Susan J M; Veldhuijzen, Irene K (2021-01-14)
    • Determinants associated with viable genital or rectal bacterial load (FemCure).

      Janssen, Kevin J H; Wolffs, Petra F G; Hoebe, Christian J P A; Heijman, Titia; Götz, Hannelore M; Bruisten, Sylvia M; Schim van der Loeff, Maarten; de Vries, Henry J; Dukers-Muijrers, Nicole H T M (2021-01-13)
      Chlamydia trachomatis (CT) is routinely diagnosed by nucleic acid amplification tests (NAATs), which are unable to distinguish between nucleic acids from viable and non-viable CT organisms.
    • Biowaiver Monographs for Immediate Release Solid Oral Dosage Forms: Metformin Hydrochloride.

      Metry, Melissa; Shu, Yan; Abrahamsson, Bertil; Cristofoletti, Rodrigo; Dressman, Jennifer B; Groot, D W; Parr, Alan; Langguth, Peter; Shah, Vinod P; Tajiri, Tomokazu; et al. (2021-01-12)
    • Modeling multi-level survival data in multi-center epidemiological cohort studies: Applications from the ELAPSE project.

      Samoli, Evangelia; Rodopoulou, Sophia; Hvidtfeldt, Ulla A; Wolf, Kathrin; Stafoggia, Massimo; Brunekreef, Bert; Strak, Maciej; Chen, Jie; Andersen, Zorana J; Atkinson, Richard; et al. (2021-01-12)
      We evaluated methods for the analysis of multi-level survival data using a pooled dataset of 14 cohorts participating in the ELAPSE project investigating associations between residential exposure to low levels of air pollution (PM2.5 and NO2) and health (natural-cause mortality and cerebrovascular, coronary and lung cancer incidence).