• Cross-border spread of - and -positive : a European collaborative analysis of whole genome sequencing and epidemiological data, 2014 to 2019.

      Ludden, Catherine; Lötsch, Felix; Alm, Erik; Kumar, Narender; Johansson, Karin; Albiger, Barbara; Huang, Te-Din; Denis, Olivier; Hammerum, Anette M; Hasman, Henrik; et al. (2020-05-01)
      Analysis of sequencing data for 143 blaNDM-1- and blaOXA-48-positive Klebsiella pneumoniae isolates from 13 European national collections and the public domain resulted in the identification of 15 previously undetected multi-country transmission clusters. For 10 clusters, cases had prior travel/hospitalisation history in countries outside of the European Union including Egypt, Iran, Morocco, Russia, Serbia, Tunisia and Turkey. These findings highlight the benefit of European whole genome sequencing-based surveillance and data sharing for control of antimicrobial resistance.
    • A VIM-1 positive strain in a near-drowning patient: evidence for interspecies plasmid transfer within the patient.

      Bosch, Thijs; Schade, Rogier; Landman, Fabian; Schouls, Leo; Dijk, Karin van (2019-09-01)
      Aim: To show that a strain of Aeromonas hydrophila became resistant to carbapenems by interspecies transfer of a plasmid using long-read sequencing. Material & methods: Whole genome sequencing of the four isolates was done using Illumina Hiseq, while the plasmid was reconstructed using the MinION sequencer. The resistome was identified with ResFinder. Results: Whole genome sequencing and long-read sequencing showed that all isolates carried a blaVIM-1 gene located on a 165 kb incA/C plasmid. ResFinder confirmed that the resistome of the plasmid, comprising 13 resistance genes, was identical within all isolates. Discussion: Long-read sequencing using the MinION successfully reconstructed a plasmid that was identical in all isolates, providing evidence for horizontal gene transfer of this blaVIM-1 gene carrying plasmid within the patient.