• 20 Years of ICF-International Classification of Functioning, Disability and Health: Uses and Applications around the World.

      Leonardi, Matilde; Lee, Haejung; Kostanjsek, Nenad; Fornari, Arianna; Raggi, Alberto; Martinuzzi, Andrea; Yáñez, Manuel; Almborg, Ann-Helene; Fresk, Magdalena; Besstrashnova, Yanina; et al. (2022-09-08)
    • Towards best use and regulatory acceptance of generic physiologically based kinetic (PBK) models for in vitro-to-in vivo extrapolation (IVIVE) in chemical risk assessment.

      Najjar, Abdulkarim; Punt, Ans; Wambaugh, John; Paini, Alicia; Ellison, Corie; Fragki, Styliani; Bianchi, Enrica; Zhang, Fagen; Westerhout, Joost; Mueller, Dennis; et al. (2022-09-05)
    • Air pollution from livestock farms and the oropharyngeal microbiome of COPD patients and controls.

      van Kersen, Warner; Bossers, Alex; de Steenhuijsen Piters, Wouter A A; de Rooij, Myrna M T; Bonten, Marc; Fluit, Ad C; Heederik, Dick; Paganelli, Fernanda L; Rogers, Malbert; Viveen, Marco; et al. (2022-09-05)
      Air pollution from livestock farms is known to affect respiratory health of patients with chronic obstructive pulmonary disease (COPD). The mechanisms behind this relationship, however, remain poorly understood. We hypothesise that air pollutants could influence respiratory health through modulation of the airway microbiome. Therefore, we studied associations between air pollution exposure and the oropharyngeal microbiota (OPM) composition of COPD patients and controls in a livestock-dense area. Oropharyngeal swabs were collected from 99 community-based (mostly mild) COPD cases and 184 controls (baseline), and after 6 and 12 weeks. Participants were non-smokers or former smokers. Annual average livestock-related outdoor air pollution at the home address was predicted using dispersion modelling. OPM composition was analysed using 16S rRNA-based sequencing in all baseline samples and 6-week and 12-week repeated samples of 20 randomly selected subjects (n = 323 samples). A random selection of negative control swabs, taken every sampling day, were also included in the downstream analysis. Both farm-emitted endotoxin and PM10 levels were associated with increased OPM richness in COPD patients (p < 0.05) but not in controls. COPD case-control status was not associated with community structure, while correcting for known confounders (multivariate PERMANOVA p > 0.05). However, members of the genus Streptococcus were more abundant in COPD patients (Benjamini-Hochberg adjusted p < 0.01). Moderate correlation was found between ordinations of 20 subjects analysed at 0, 6, and 12 weeks (Procrustes r = 0.52 to 0.66; p < 0.05; Principal coordinate analysis of Bray-Curtis dissimilarity), indicating that the OPM is relatively stable over a 12 week period and that a single sample sufficiently represents the OPM. Air pollution from livestock farms is associated with OPM richness of COPD patients, suggesting that the OPM of COPD patients is susceptible to alterations induced by exposure to air pollutants.
    • Neuromodulatory and neurotoxic effects of e-cigarette vapor using a realistic exposure method.

      Staal, Yvonne C M; Li, Yixuan; Gerber, Lora-Sophie; Fokkens, Paul; Cremers, Hans; Cassee, Flemming R; Talhout, Reinskje; Westerink, Remco H S; Heusinkveld, Harm J (2022-09-02)
      The most direct effects of inhaled harmful constituents are the effects on the airways. However, inhaled compounds can be rapidly absorbed and subsequently result in systemic effects. For example, e-cigarette vapor has been shown to evoke local effects in the lung, although little is known about subsequent effects in secondary target organs such as the brain. Traditionally, such effects are tested using in vivo models. As an alternative, we have combined two in vitro systems, which are Air-Liquid-Interface (ALI) cultured alveolar cells (A549) and rat primary cortical cultures grown on multi-well microelectrode arrays. This allows us to assess the neurological effects of inhaled compounds. We have used exposure to e-cigarette vapor, containing nicotine, menthol, or vanillin to test the model. Our results show that ALI cultured A549 cells respond to the exposure with the production of cytokines (IL8 and GROalpha). Furthermore, nicotine, menthol, and vanillin were found on the basolateral side of the cell culture, which indicates their translocation. Upon transfer of the basolateral medium to the primary cortical culture, exposure-related changes in spontaneous electrical activity were observed correlating with the presence of e-liquid components in the medium. These clear neuromodulatory effects demonstrate the feasibility of combining continuous exposure of ALI cultured cells with subsequent exposure of neuronal cells to assess neurotoxicity. Although further optimization steps are needed, such a combination of methods is important to assess the neurotoxic effects of inhaled compounds realistically. As such, an approach like this could play a role in future mechanism-based risk assessment strategies.
    • The detection of monkeypox virus DNA in wastewater samples in the Netherlands.

      de Jonge, Eline F; Peterse, Céline M; Koelewijn, Jaap M; van der Drift, Anne-Merel R; van der Beek, Rudolf F H J; Nagelkerke, Erwin; Lodder, Willemijn J (2022-08-31)
    • International expert group collaboration for developing an adverse outcome pathway for radiation induced leukaemia.

      Klokov, Dmitry; Applegate, Kimberly; Badie, Christophe; Brede, Dag Anders; Dekkers, Fieke; Karabulutoglu, Melis; Le, Yevgeniya; Rutten, Eric Andreas; Lumniczky, Katalin; Gomolka, Maria (2022-08-30)
    • Developing human biomonitoring as a 21st century toolbox within the European exposure science strategy 2020-2030.

      Zare Jeddi, Maryam; Hopf, Nancy B; Louro, Henriqueta; Viegas, Susana; Galea, Karen S; Pasanen-Kase, Robert; Santonen, Tiina; Mustieles, Vicente; Fernandez, Mariana F; Verhagen, Hans; et al. (2022-08-28)
    • Characterization of the virulence of three novel clade 2 Clostridioides (Clostridium) difficile strains and a two-year screening in animals and humans in Brazil.

      Diniz, Amanda Nadia; Moura, Loren Nery Fontoura; Cruz, Diogo Soares Gonçalves; Oliveira Junior, Carlos Augusto; Figueiredo, Henrique César Pereira; Cunha, João Luís Reis; Vilela, Eduardo Garcia; Kuijper, Edward J; Wilcox, Mark H; Lobato, Francisco Carlos Faria; et al. (2022-08-26)
    • Green Chemistry Based Gold Nanoparticles Synthesis Using the Marine Bacterium PBCW2 and Their Multitudinous Activities.

      Cherian, Tijo; Maity, Debasis; Rajendra Kumar, Ramasamy T; Balasubramani, Govindasamy; Ragavendran, Chinnasamy; Yalla, Suneelkumar; Mohanraju, Raju; Peijnenburg, Willie J G M (2022-08-26)
      Green chemistry has paved an 'avant-garde avenue' in the production and fabrication of eco-friendly stable nanoparticles employing the utilization of biological agents. In the present study we present the first report on the potential of the marine bacterium Lysinibacillus odysseyi PBCW2 for the extracellular production of gold nanoparticles (AuNPs). Utilizing a variety of methods, AuNPs in the cell-free supernatant of L. odysseyi (CFS-LBOE) were identified and their antioxidant, antibacterial, and dye-degrading properties were examined. The visual coloring of the reaction mixture to a ruby red hue showed the production of LBOE-AuNPs; validated by means of XRD, TEM, SEM, XRD, DLS, TGA, and FT-IR analysis. Additionally, the 2,2-diphenyl-1-picrylhydrazyl technique and the well diffusion assay were used to examine their dose-dependent antioxidant and antibacterial activity. These biogenic LBOE-AuNPs showed 91% dye degradation efficiency during catalytic reduction activity on BTB dye, demonstrating their versatility as options for heterogeneous catalysis.
    • Genetic Characteristics of Methicillin-Resistant Staphylococcus argenteus Isolates Collected in the Dutch National MRSA Surveillance from 2008 to 2021.

      Witteveen, Sandra; Hendrickx, Antoni P A; de Haan, Angela; Notermans, Daan W; Landman, Fabian; van Santen-Verheuvel, Marga G; de Greeff, Sabine C; Kuijper, Ed J; van Maarseveen, Noortje M; Vainio, Saara; et al. (2022-08-25)
      Staphylococcus argenteus is a recently described member of the Staphylococcus aureus complex (SAC) and is associated with human disease. The frequency and intensity of infections caused by S. argenteus are similar to those of Staphylococcus aureus. S. argenteus can harbor antibiotic resistance genes and a variety of virulence factors analogous to methicillin-resistant S. aureus (MRSA). The aim of our study was to analyze a collection of isolates in the Dutch national MRSA surveillance from January 2008 until March 2021 that were nontypeable by multilocus variable-number tandem-repeat analysis (MLVA). Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-ToF MS) was used for identifying the S. argenteus isolates, and whole-genome sequencing and SeqSphere were used to generate an in-house whole-genome multilocus sequence typing (wgMLST) scheme for typing the isolates. Furthermore, the presence of antibiotic resistance genes, replicons, and virulence genes was determined. Of 52,467 isolates submitted as MRSA from January 2008 until March 2021, 64 isolates (0.12%) were nontypeable with MLVA, and 54 of them were identified with mass spectrometry (MALDI-ToF MS) as S. argenteus. It appeared in retrospect that the first methicillin-resistant S. argenteus (MRSArg) was already submitted in 2008. An in-house-developed S. argenteus wgMLST scheme revealed that S. argenteus isolates clustered in 5 genomic groups which were characterized by distinct MLST types, resistomes, plasmid replicon families, and virulence factors. All but one isolate carried the staphylococcal chromosomal cassette mec (SCCmec) type IV harboring the methicillin resistance gene mecA and represent MRSArg. Most of the isolates with SCCmec subtype IVc(2B) had a trimethoprim resistance gene, dfrG, and harbored a blaZ-carrying plasmid, and most MRSArg isolates have the immune-modulating genes scn and sak. Nine of the 47 isolates carried enterotoxin-encoding genes seg, sei, sem, seo, and seu, which might be able to cause food poisoning. In some persons there was long-term persistence of MRSArg, and there were several genetically related MRSArg isolates in people living in close proximity, suggesting direct human-human transmission. IMPORTANCE We show that MRSArg has been circulating in the Netherlands since at least 2008. Although MRSArg is distinct from MRSA, it has a comparable population structure and carries similar resistance and virulence genes. The Dutch national MRSA surveillance has been expanded to include other methicillin-resistant members of the S. aureus complex, such as S. argenteus and Staphylococcus schweitzeri.
    • Identifying platelet-derived factors as amplifiers of B. burgdorferi-induced cytokine production.

      Kerstholt, Mariska; van de Schoor, Freek R; Oosting, Marije; Moorlag, Simone J C F M; Li, Yang; Jaeger, Martin; van der Heijden, Wouter A; Tunjungputri, Rahajeng N; Dos Santos, Jéssica C; Vrijmoeth, Hedwig D; et al. (2022-08-24)
    • A randomized crossover trial assessing time of day snack consumption and resulting postprandial glycemic response in a real-life setting among healthy adults.

      Timmer, Ruth; Bogaardt, Laurens; Brummelhuis, Walter J; van Oostrom, Conny T; van Kerkhof, Linda W M; Wong, Albert; de Valk, Harold W; Ocké, Marga C; van der Maaden, Tessa; Dollé, Martijn E T (2022-08-24)
    • The process evaluation of a citizen science approach to design and implement workplace health promotion programs.

      Lelie, Lisa; van der Molen, Henk F; van den Berge, Mandy; van der Feltz, Sophie; van der Beek, Allard J; Hulshof, Carel T J; Proper, Karin I (2022-08-24)
    • Challenges in Predicting the Change in the Cumulative Exposure of New Tobacco and Related Products Based on Emissions and Toxicity Dose-Response Data.

      Staal, Yvonne C M; Bil, Wieneke; Bokkers, Bas G H; Soeteman-Hernández, Lya G; Stephens, W Edryd; Talhout, Reinskje (2022-08-24)
    • Methodological Approaches for Risk Assessment of Tobacco and Related Products.

      Staal, Yvonne C M; Bos, Peter M J; Talhout, Reinskje (2022-08-24)
    • GREENER Pharmaceuticals for More Sustainable Healthcare

      Moermond, CTA; Puhlmann, N; Brown, AR; Owen, SF; Venhuis, BJ; et al. (2022-08-23)
    • Outpatient convalescent plasma therapy for high-risk patients with early COVID-19. A randomized placebo-controlled trial.

      Gharbharan, Arvind; Jordans, Carlijn; Zwaginga, Lisa; Papageorgiou, Grigorios; van Geloven, Nan; van Wijngaarden, Peter; den Hollander, Jan; Karim, Faiz; van Leeuwen-Segarceanu, Elena; Soetekouw, Robert; et al. (2022-08-22)
    • Habit formation of preventive behaviours during the COVID-19 pandemic: a longitudinal study of physical distancing and hand washing.

      Zhang, Chao; Adriaanse, Marieke A; Potgieter, Renske; Tummers, Lars; de Wit, John; Broersen, Jan; de Bruin, Marijn; Aarts, Henk (2022-08-20)
    • Development of a cell line-based in vitro assay for assessment of Diphtheria, Tetanus and acellular Pertussis (DTaP)-induced inflammasome activation.

      Vandebriel, Rob J; Stalpers, Coen A L; Vermeulen, Jolanda P; Remkes, Mariska; Schmelter, Marit; Broere, Femke; Hoefnagel, Marcel H N (2022-08-20)