Now showing items 21-40 of 2892

    • Instability of Acylcarnitines in Stored Dried Blood Spots: The Impact on Retrospective Analysis of Biomarkers for Inborn Errors of Metabolism.

      van Rijt, Willemijn J; Schielen, Peter C J I; Özer, Yasemin; Bijsterveld, Klaas; van der Sluijs, Fjodor H; Derks, Terry G J; Heiner-Fokkema, M Rebecca (2020-11-02)
    • Guidelines for analysis of low-frequency antigen-specific T cell results: Dye-based proliferation assay vs H-thymidine incorporation.

      Di Blasi, Daniela; Claessen, Iris; Turksma, Annelies W; van Beek, Josine; Ten Brinke, Anja (2020-11-02)
      It is generally recognized that dysregulation of the immune system plays a critical role in many diseases, including autoimmune diseases and cancer. T cells play a crucial role in maintaining self-tolerance, while loss of immune tolerance and T cell activation can lead to severe inflammation and tissue damage. T cell responses have a key role in the effectiveness of vaccination strategies and immunomodulating therapies. Immunomonitoring methods have the ability to elucidate immunological processes, monitor the development of disease and assess therapeutic effects. In this respect, it is of particular interest to evaluate antigen (Ag)-specific T cells by determining their frequency, type and functionality in cellular assays. Nevertheless, Ag-specific T cells are detected infrequently in most diseases using current techniques. Many efforts have been made to develop more sensitive, reproducible, and reliable methods for Ag-specific T cell detection. It has been found that analysis of cellular proliferation can be a useful tool to determine the presence and frequency of Ag-specific T cell and to provides insight into modulation of the T cell response by a specific antigen or therapy. However, the selection of a cut-off value for a positive response and therefore a more accurate interpretation of the data, continues to be a major concern. Here, we provide guidelines to select a proper cut-off for monitoring of Ag-specific CD4+ T cell responses. In vitro Ag-stimulation has been assessed with two methods; a dye-based proliferation assay and 3H-thymidine-based assay. Two cut-off approaches are compared; mean and variance of control wells, and the stimulation index. By evaluating the proliferative response to the in vitro Ag-stimulation using these two methods, we demonstrate the importance of taking into consideration the variability of the control wells to distinguish a positive from a false positive response.
    • Dynamics of in azole-fungicide-containing plant waste, the Netherlands, 2016-2017.

      Zhang, Jianhua; Lopez Jimenez, Lidia; Snelders, Eveline; Debets, Alfons J M; Rietveld, Anton G; Zwaan, Bas J; Verweij, Paul E; Schoustra, Sijmen E (2020-10-30)
      The treatment of patients suffering from Aspergillus diseases is hampered due to infections with Aspergillus fumigatus that are already resistant to medical azoles. Previous work has suggested that A. fumigatus likely gains resistance through environmental azole exposure in so-called hotspots. Here, we investigated A. fumigatus resistance dynamics over time, at three sites at which farmers used azole fungicides for crop protection. Over 16 months 114 samples were taken from stockpiles of decaying plant waste. A. fumigatus and azole fungicide residues were ubiquitously present in the plant waste. On average 105A. fumigatus CFU/g was recovered of which roughly half were itraconazole and tebuconazole resistant. Similar tandem repeat-mediated resistance mechanisms were found in colonies cultured from plant waste as reported in clinical azole-resistant isolates. Our results show a consistent high burden of azole-resistant A. fumigatus in azole-containing plant waste and underscores the need to further investigate resistance-reducing interventions and transmission routes.ImportanceAspergillus fumigatus is consistently present independently on season at a high abundance in plant-waste material throughout sampling period. Our study confirmed that long-term storage of azole-containing decaying plant material indeed be considered hotspots, which can sustain resistance development and maintenance in A. fumigatus Roughly half of individual isolates were azole-resistant and carry genetic mutations that are highly similar to those found in patients with azole-resistant invasive aspergillosis. Our work suggests that environmental sources of azole resistance in A. fumigatus might be important, underscoring the need for further studies on environment-to-patient transmission routes.
    • Influenza-like Illness Exacerbates Pneumococcal Carriage in Older Adults.

      Miellet, Willem R; van Veldhuizen, Janieke; Nicolaie, Mioara A; Mariman, Rob; Bootsma, Hester J; Bosch, Thijs; Rots, Nynke Y; Sanders, Elisabeth A M; van Beek, Josine; Trzciński, Krzysztof (2020-10-30)
    • Effects of protein and calorie restriction on the metabolism and toxicity profile of irinotecan in cancer patients.

      de Man, Femke M; van Eerden, Ruben A G; van Doorn, Gerdien M; Oomen-de Hoop, Esther; Koolen, Stijn L W; Olieman, Joanne F; de Bruijn, Peter; Veraart, Joris N; van Halteren, Henk K; Sandberg, Yorick; et al. (2020-10-29)
      Preclinical data suggests that protein and calorie restriction (PCR) might improve treatment tolerability without impairing antitumor effect. Therefore, we have studied the influence of PCR on irinotecan pharmacokinetics and toxicity. In this cross-over trial, patients with liver metastases of solid tumors were included and randomized to treatment with irinotecan preceded by 5 days of PCR (~30% caloric and ~70% protein restriction) during the 1st cycle and a 2nd cycle preceded by a normal diet (ND) or vice versa. Pharmacokinetic blood sampling and biopsies of both healthy liver (HL) and liver metastasis (LM) were performed. Primary endpoint was the relative difference in geometric means for the active metabolite SN-38 concentration in HL analyzed by a linear mixed model. No significant differences were seen in irinotecan (+16.8%, P=0.22) and SN-38 (+9.8%, P=0.48) concentrations between PCR and ND in HL, as well as in LM (irinotecan: -38.8%, P=0.05 and SN-38: -13.8%, P=0.50). PCR increased irinotecan plasma AUC0-24h with 7.1% (P=0.04) compared to ND, while the SN-38 plasma AUC0-24h increased with 50.3% (P<0.001). Grade ≥3 toxicity was not increased during PCR vs ND (P=0.69). No difference was seen in neutropenia grade ≥3 (47% vs 32% P=0.38), diarrhea grade ≥3 (5% vs 21% P=0.25) and febrile neutropenia (5% vs 16% P=0.50) during PCR vs ND. In conclusion, plasma SN-38 exposure increased dramatically after PCR, while toxicity did not change. PCR did not alter the irinotecan and SN-38 exposure in HL and LM. PCR might therefore potentially improve the therapeutic window in patients treated with irinotecan.
    • Proximity to livestock farms and exposure to livestock-related particulate matter are associated with lower probability of medication dispensing for obstructive airway diseases.

      Post, Pim M; Houthuijs, Danny; Sterk, Hendrika A M; Marra, Marten; van de Kassteele, Jan; van Pul, Addo; Smit, Lidwien A M; van der Hoek, Wim; Lebret, Erik; Hogerwerf, Lenny (2020-10-28)
      A nationwide population-based cross-sectional study was conducted among 7,735,491 persons, with data on the dispensing of drugs for obstructive airway diseases in the Netherlands in 2016. Exposure was based on distances between home addresses and farms and on modelled atmospheric particulate matter (PM10) concentrations from livestock farms. Data were analysed for different regions by logistic regression analyses and adjusted for several individual-level variables, as well as modelled PM10 concentration of non-farm-related air pollution. Results for individual regions were subsequently pooled in meta-analyses.
    • The shared socio-economic pathway (SSP) greenhouse gas concentrations and their extensions to 2500.

      Meinshausen, M; Nicholls, ZRJ; Lewis, J; Gidden, MJ; Vogel, E; Freund, M; Beyerle, U; Gessner, C; Nauels, A; Bauer, N; et al. (2020-10-26)
    • A multifactorial approach for surveillance of Shigella spp. and entero-invasive Escherichia coli is important for detecting (Inter)national clusters

      van den Beld, MJC; Reubsaet, FAG; Pijnacker, R; Harpal, A; Kuiling, S; Heerkens, EM; Hoeve-Bakker, BJA; Nooemen, RCEA; Hendriks, ACA; Borst, D; et al. (2020-10-26)
    • Integration of local knowledge and data for spatially quantifying ecosystem services in the Hoeksche Waard, the Netherlands.

      Paulin, MJ; Rutgers, M; de Nijs, T; Hendriks, AJ; Koopman, KR; van Buul, T; Frambach, M; Sardano, G; Breure, AM (2020-10-26)
    • Different Long-Term Duration of Seroprotection against in Adolescents and Middle-Aged Adults after a Single Meningococcal ACWY Conjugate Vaccination in The Netherlands.

      Ohm, Milou; van Rooijen, Debbie M; Bonačić Marinović, Axel A; van Ravenhorst, Mariëtte B; van der Heiden, Marieke; Buisman, Anne-Marie; Sanders, Elisabeth A M; Berbers, Guy A M (2020-10-25)
      Neisseria meningitidis is often asymptomatically carried in the nasopharynx but may cause invasive meningococcal disease, leading to morbidity and mortality. Meningococcal conjugate vaccinations induce functional protective antibodies against capsular antigens, but seroprotection wanes over time. We measured functional antibody titers five years after administration of a single dose of the meningococcal ACWY-polysaccharide-specific tetanus toxoid-conjugated (MenACWY-TT) vaccine in adolescents and middle-aged adults in the Netherlands, using the serum bactericidal antibody with baby rabbit complement (rSBA) assay. Protection was defined as rSBA titer ≥8. The meningococcal ACWY-specific serum IgG concentrations were measured with a multiplex immunoassay. Duration of protection was estimated by a bi-exponential decay model. Sufficient protection for MenC, MenW, and MenY was achieved in 94-96% of the adolescents five years postvaccination, but, in middle-aged adults, only in 32% for MenC, 65% for MenW and 71% for MenY. Median duration of protection for MenCWY was 4, 14, and 21 years, respectively, in middle-aged adults, while, in adolescents, it was 32, 98, and 33 years. Our findings suggest that adolescents, primed in early childhood with MenC conjugate vaccination, remain sufficiently protected after a single dose of MenACWY-TT vaccine. Middle-aged adults without priming vaccination show fast waning of antibodies, particularly MenC, for which protection is lost after four years.
    • Digging into Toxoplasma gondii infections via soil: A quantitative microbial risk assessment approach.

      Deng, Huifang; Exel, Kitty E; Swart, Arno; Bonačić Marinović, Axel A; Dam-Deisz, Cecile; van der Giessen, Johanna W B; Opsteegh, Marieke (2020-10-24)
    • Knowledge, attitudes, beliefs, and stigma related to latent tuberculosis infection: a qualitative study among Eritreans in the Netherlands.

      Spruijt, Ineke; Haile, Dawit Tesfay; van den Hof, Susan; Fiekert, Kathy; Jansen, Niesje; Jerene, Degu; Klinkenberg, Eveline; Leimane, Ieva; Suurmond, Jeanine (2020-10-23)
    • Do background levels of the pesticide pirimiphosmethyl in plant-based aquafeeds affect food safety of farmed Atlantic salmon?

      Berntssen, Marc H G; Hoogenveen, Rudolf; Rosenlund, Grethe; Garlito, Borja; Zeilmaker, Marco J (2020-10-20)
      The substitution of fish oil and fishmeal with plant-based ingredients in commercial aquafeeds for Atlantic salmon, may introduce novel contaminants that have not previously been associated with farmed fish. The organophosphate pesticide pirimiphos-methyl (PM) is one of the novel contaminants that is most prevalent in commercial salmon feed. In this study, the feed-to-fillet transfer of dietary PM and its main metabolites was investigated in Atlantic salmon fillet. Based on the experimental determined PM and metabolite uptake, metabolisation, and elimination kinetics, a physiologically based toxicokinetic (PBTK) compartmental model was developed. Fish fed PM had a relatively low (~4%) PM retention and two main metabolites (2-DAMP and Desethyl-PM) were identified in liver, muscle, kidney and bile. The absence of more metabolised forms of 2-DAMP and Desethyl-PM in Atlantic salmon indicates different metabolism in cold-water fish compared to previous studies on ruminants. The model was used to simulate the long term (>1.5 years) feed-to-fillet transfer of PM + metabolite in Atlantic salmon under realistic farming conditions including seasonal fluctuations in feed intake, growth, and fat deposition in muscle tissue. The model predictions show that with the constant presence of the highest observed PM concentration in commercial salmon feed, fillet PM+ metabolite levels were approximately 5 nmol kg-1, with highest levels for the metabolite 2-DAMP. No EU maximum residue levels (MRL) for PM and its main metabolites exist in seafood to date, but the predicted levels were lower than the MRL for PM in swine of 32.7 nmol kg-1.
    • How age and infection history shape the antigen-specific CD8 T-cell repertoire: Implications for vaccination strategies in older adults.

      Lanfermeijer, Josien; Borghans, José A M; van Baarle, Debbie (2020-10-20)
      Older adults often show signs of impaired CD8+ T-cell immunity, reflected by weaker responses against new infections and vaccinations, and decreased protection against reinfection. This immune impairment is in part thought to be the consequence of a decrease in both T-cell numbers and repertoire diversity. If this is indeed the case, a strategy to prevent infectious diseases in older adults could be the induction of protective memory responses through vaccination at a younger age. However, this requires that the induced immune responses are maintained until old age. It is therefore important to obtain insights into the long-term maintenance of the antigen-specific T-cell repertoire. Here, we review the literature on the maintenance of antigen-experienced CD8+ T-cell repertoires against acute and chronic infections. We describe the complex interactions that play a role in shaping the memory T-cell repertoire, and the effects of age, infection history, and T-cell avidity. We discuss the implications of these findings for the development of new vaccination strategies to protect older adults.
    • A Semi-Automated Workflow for FAIR Maturity Indicators in the Life Sciences.

      Ammar, Ammar; Bonaretti, Serena; Winckers, Laurent; Quik, Joris; Bakker, Martine; Maier, Dieter; Lynch, Iseult; van Rijn, Jeaphianne; Willighagen, Egon (2020-10-20)
    • Author Correction: Rapid SARS-CoV-2 whole-genome sequencing and analysis for informed public health decision-making in the Netherlands.

      Oude Munnink, Bas B; Nieuwenhuijse, David F; Stein, Mart; O'Toole, Áine; Haverkate, Manon; Mollers, Madelief; Kamga, Sandra K; Schapendonk, Claudia; Pronk, Mark; Lexmond, Pascal; et al. (2020-10-20)
    • Exploring the Team Climate of Health and Social Care Professionals Implementing Integrated Care for Older People in Europe.

      MacInnes, Julie; Gadsby, Erica; Reynolds, Jillian; Mateu, Nuri Cayuelas; Lette, Manon; Ristl, Christina; Billings, Jenny (2020-10-19)
      Team climate describes shared perceptions of organisational policies, practices and procedures. A positive team climate has been linked to better interprofessional collaboration and quality of care. Most studies examine team climate within health or social care organisations. This study uniquely explores the team climate of integrated health and social care teams implementing integrated care initiatives for older people in thirteen sites across seven European countries, and examines the factors which contribute to the development of team climate.
    • Rebuttal to the letters to the editors by Terry et al. and Sewell et al. regarding.

      Heringa, Minne B; Cnubben, Nicole H P; Slob, Wout; Pronk, Marja E J; Muller, Andre; Woutersen, Marjolijn; Hakkert, Betty C (2020-10-19)