EU Interlaboratory comparison study primary production XVI (2013) : Detection of Salmonella in chicken faeces adhering to boot socks
Series/Report no.
RIVM report 330604031Type
OnderzoeksrapportLanguage
enMetadata
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EU Interlaboratory comparison study primary production XVI (2013) : Detection of Salmonella in chicken faeces adhering to boot socksTranslated Title
EU Ringonderzoek primaire productie XVI (2013) : Detectie van Salmonella in overschoenen met kippenmestPubliekssamenvatting
In 2013 waren alle 36 Nationale Referentie Laboratoria (NRL's) in de Europese Unie in staat om hoge en lage concentraties Salmonella in een stal met leghennen (kippenmest) aan te tonen. Ze behaalden direct het gewenste niveau. In totaal hebben de laboratoria in 96 procent van de besmette monsters Salmonella opgespoord. Dit blijkt uit het zestiende ringonderzoek met materiaal van de dieren (zoals uitwerpselen) dat werd georganiseerd door het referentielaboratorium van de Europese Unie voor Salmonella (EURLSalmonella). Voor dit soort onderzoek zijn monsters van de uitwerpselen van kippen verzameld door met overschoenen door de stal te 'wandelen' (omgevingsmateriaal).Ringonderzoek verplicht voor Europese lidstaten
Het onderzoek is in maart 2013 gehouden. Alle NRL's van de Europese lidstaten die verantwoordelijk zijn voor de opsporing van Salmonella in dierlijke mest, zijn verplicht om aan het onderzoek deel te nemen. Het EURL-Salmonella is gevestigd bij het Nederlandse Rijksinstituut voor Volksgezondheid en Milieu (RIVM).
De laboratoria toonden de Salmonella-bacterie in de overschoenen met kippenmest aan met behulp van de internationaal voorgeschreven analysemethode (MSRV). Elk laboratorium kreeg een pakket toegestuurd met overschoenen waaraan kippenmest zat met Salmonella in twee verschillende concentraties of zonder Salmonella. De laboratoria dienden de monsters volgens een protocol te onderzoeken op de aanwezigheid van Salmonella.
Nieuwe werkwijzen
Voor het eerst is het te onderzoeken materiaal (matrix) op het laboratorium van het EURL-Salmonella kunstmatig besmet met een verdunde cultuur van een Salmonella Typhimurium. Het laboratorium van het EURL-Salmonella heeft voor dit soort studies onderzocht hoe de monsters op deze wijze optimaal kunnen worden aangeleverd. De NRL's vinden deze werkwijze positief, omdat zijzelf niet meer de monsters met de Salmonella hoeven samen te voegen; dit was in eerdere studies wel het geval. Deze werkwijze wordt daarom voorgezet, al wordt per studie bekeken of het haalbaar is. Een andere vernieuwing is dat de deelnemende laboratoria hun bevindingen via internet konden aanleveren. De NRL's vonden ook dit een verbetering, en voor het analyserend EURL zijn de gegevens eenvoudiger te analyseren. Besloten is deze werkwijze te optimaliseren en voort te zetten.
In 2013, all 36 National Reference Laboratories (NRLs) in the European Union were able to detect high and low levels of Salmonella in chicken faeces collected from stables with laying hens. The laboratories achieved the desired level of good performance immediately. The laboratories detected Salmonella in 96% of the contaminated samples. This is evident from the 16th interlaboratory comparison study of primary production samples (such as chicken faeces), which was organized by the European Union Reference Laboratory for Salmonella (EURL-Salmonella). In this study, environmental material was collected by researchers walking through the stable wearing overshoes (boot socks).
Interlaboratory comparison study obligatory for EU Member States
The study was conducted in March 2013. Participation was obligatory for all EU Member State NRLs which are responsible for the detection of Salmonella in samples from primary production. EURL-Salmonella is part of the Dutch National Institute for Public Health and the Environment (RIVM).
The laboratories used the internationally prescribed Modified Semi-solid Rappaport-Vassiliadis (MSRV) method to detect the presence of Salmonella in chicken faeces adhering to boot socks. Each laboratory received a package of boot socks containing chicken faeces with two different concentrations of Salmonella, or containing no Salmonella at all. The laboratories were required to analyse the samples for the presence of Salmonella in accordance with the study protocol.
New procedures
For the first time, the samples (matrix) were artificially contaminated with a diluted culture of Salmonella Typhimurium at the EURL-Salmonella laboratory. The EURL-Salmonella laboratory investigated the optimal sample delivery procedure for this type of study. The procedure used was positively received by the NRLs because they themselves were no longer required to combine the Salmonella samples, as was the case in previous studies. This procedure will therefore continue to be used in future studies, although its feasibility will be assessed for each study. A further innovation was that the participating laboratories were able to submit their findings via the Internet. This change was also positively received by the NRLs, and made it easier for the EURL to analyse the data. It was decided to optimize this procedure and to continue using it.
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European Commission (DG Sanco)Collections
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