The 21st EURL-Salmonella workshop : 9 June 2016, Saint Malo, France
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Series/Report no.RIVM report 2016-0045
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TitleThe 21st EURL-Salmonella workshop : 9 June 2016, Saint Malo, France
Translated TitleDe 21e EURL-Salmonella workshop : 9 juni 2016, Saint Malo, Frankrijk
PubliekssamenvattingDit rapport bevat een bundeling van verslagen van de presentaties van de 21e jaarlijkse workshop voor de Europese Nationale Referentie Laboratoria (NRL's) voor Salmonella (9 juni 2016). Het doel van de workshop is dat het overkoepelende orgaan, het Europese Referentie Laboratorium (EURL) voor Salmonella en de NRL's informatie uitwisselen.
Een terugkerend onderwerp is de ringonderzoeken die het EURL jaarlijks organiseert en waarmee de kwaliteit van de NRL laboratoria wordt gecontroleerd. De NRL's hadden er in 2015 geen problemen mee om Salmonella in ei te vinden. In dit rapport staan de ringonderzoeken kort beschreven. Een uitgebreidere weergave van de resultaten wordt per ringonderzoek gepubliceerd.
Een aantal verslagen geeft informatie over het gebruik van moleculaire technieken om Salmonella te typeren. Met deze technieken wordt het DNA van de bacterie aangetoond. Deze technieken worden steeds vaker gebruikt bij het opsporen van ziekmakende bacteriën in voedsel, dieren en bij de mens. Iedere bacteriestam heeft namelijk een eigen unieke moleculaire typering.
Opslag moleculaire typering resultaten
De European Food Safetey Authority (EFSA) geeft verslag van een databank die sinds begin 2016 beschikbaar is. In deze databank kunnen alle Europese landen moleculaire typering resultaten van Salmonella opslaan. Dit geeft informatie of een bepaalde ziekmakende bacteriestam in meerdere landen en producten voorkomt.
De organisatie van de workshop is in handen van het EURL voor Salmonella, dat onderdeel is van het RIVM. De hoofdtaak van het EURL-Salmonella is toezien op de kwaliteit van de nationale referentielaboratoria voor deze bacterie in Europa.
This report contains a summary of the presentations given at the 21st annual workshop for the European National Reference Laboratories (NRLs) for Salmonella (9 June 2016). The aim of the workshop is to facilitate the exchange of information on the activities of the NRLs and the European Union Reference Laboratory for Salmonella (EURL-Salmonella).
Annual ring trials
A recurring item at the workshops is the presentation of the results of the annual ring trials organised by the EURL. These ring trials give information on the quality of the NRL laboratories tested. The 2015 trial showed that all NRLs were able to detect Salmonella in whole liquid egg. Detailed information on the results per ring trial is given in separate RIVM-reports.
Several presentations provided information on the use of molecular techniques for Salmonella typing. These techniques analyse the DNA of the bacterium, and are often used to trace pathogens in food, animals or humans. Each strain has its own unique molecular typing pattern.
Storage of molecular typing results
The European Food Safety Authority (EFSA) presented a database for storage of the molecular Salmonella typing results. This database has been available since early 2016 and will make it possible to check whether a specific strain is found in different countries and products.
The annual workshop is organised by the EURL-Salmonella, part of the Dutch National Institute for Public Health and the Environment. The main task of the EURL-Salmonella is to evaluate the performance of the European NRLs in detecting and typing Salmonella in different products.
Descriptionalleen digitaal verschenen
Directorate-General for Health and Food Safety (DG-Sante)
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Thirteenth CRL-Salmonella interlaboratory comparison study (2008) on typing of Salmonella spp. : Dertiende CRL-Salmonella ringonderzoek (2008) voor de typering van Salmonella spp.Berk PA; Maas HME; de Pinna E; Mooijman KA; LZO; cib (Rijksinstituut voor Volksgezondheid en Milieu RIVMHealth Protection Agency HPA, 2010-11-04)The National Reference Laboratories (NRLs) of all 27 European Member States performed well on the 2008 quality control test on Salmonella typing. The 4 laboratories which repeated the test also obtained good scores. An analysis of the pooled results from all NRLs revealed that the NRLs taken as a whole were able to assign the correct name to 97 % of the strains tested. One NRL performed the test at a relatively late date and, consequently, its data could not be included in the group analysis. Since 1992, the NRLs have been required to participate in an annual quality control test, which consists of an interlaboratory comparison study for Salmonella typing. Each Member State designates a specific laboratory within their national boundaries to be responsible for the detection and identification of Salmonella strains from samples isolated from animals and/or food products. These laboratories are then referred to as the National Reference Laboratories. The performance of the NRLs is assessed annually based on their capability to correctly identify 20 Salmonella strains. NRLs from countries outside the European Union occasionally participate in these tests, and NRLs from 2 countries belonging to the European Free Trade Association (EFTA) took part in the 2008 test. The expertise of a number of NRLs was subjected to more severe testing by having not only to identify the 20 Salmonella strains of the quality control test but also to subtype (phage typing) various other Salmonella strains. As such, these laboratories received 10 strains of each of Salmonella Enteritidis and Salmonella Typhimurium. These NRLs typed 97 % of the S. Typhimurium strains correctly. The typing of S. Enteritidis strains proved to be more troublesome, with the NRLs typing 94 % of the strains correctly. The Community Reference Laboratory for Salmonella (CRL-Salmonella) organises this annual interlaboratory comparison study in cooperation with the Health Protection Agency in London, UK. The CRL-Salmonella is situated at the National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands.
Sixteenth EURL-Salmonella interlaboratory comparison study (2011) on typing of Salmonella spp. : Zestiende EURL-Salmonella ringonderzoek (2011) voor de typering van Salmonella spp.Jacobs-Reitsma WF; Pol-Hofstad IE; Maas HME; de Pinna E; Mooijman KA; LZO; cib (Rijksinstituut voor Volksgezondheid en Milieu RIVM, 2012-10-24)The 28 National Reference Laboratories (NRLs) of all 27 European Union (EU) Member States performed well on the 2011 quality control test on Salmonella typing. Two laboratories were found to require a follow-up study on their first test. Altogether, the EU-NRLs were able to assign the correct name to 97% of the strains tested. Other participants interlaboratory comparison study Salmonella Since 1992, the NRLs of the EU Member States have been required to participate in annual quality control tests, which consist of interlaboratory comparison studies on Salmonella. Laboratories from countries outside the European Union, like EU-candidate countries, occasionally participate in these tests on a voluntary basis. Eight additional laboratories participated in the current study. Two EUcandidate countries amongst these eight additional participants did not meet the criteria for good performance in the first round. One of them did not reach this goal in the follow-up study either. The other was not able to participate in the follow-up study; a follow-up study is not compulsory for non-EU laboratories. Each EU Member State designates a specific laboratory within their national boundaries to be responsible for the detection and identification of Salmonella strains from animals and/or food products. These laboratories are then referred to as the National Reference Laboratories. The performance of these NRLs on Salmonella typing is assessed annually, based on their capability to correctly identify 20 Salmonella strains. Phage typing Nine NRLs not only serotyped the 20 Salmonella strains of the quality control test, but also subtyped 20 additional strains by phage typing. For this, the laboratories received ten strains of Salmonella Enteritidis and ten strains of Salmonella Typhimurium. These NRLs typed 98% of the S. Typhimurium strains correctly. Of the S. Enteritidis strains, 88% were phage typed correctly. The European Union Reference Laboratory for Salmonella (EURL-Salmonella) organises this annual interlaboratory comparison study on typing of Salmonella in cooperation with the Health Protection Agency in London, UK. The EURLSalmonella is situated at the National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands.
Test results of Salmonella typing by the NRLs-Salmonella in the Member States of the EU and the EnterNet Laboratories - Collaborative study VI (2001) on typing of SalmonellaKorver H; Raes M; Maas HME; Ward LR; Wannet WJB; Henken AM; MGB; LIS (PHLS-Colindale/London, 2002-09-06)Test results of Salmonella sero- and phage typing and antimicrobial susceptibility testing by the National Reference Laboratories for Salmonella in the Member States of the European Union and the EnterNet Laboratories: Collaborative study VI (2001) for Salmonella. The sixth collaborative typing study for Salmonella was organised by the Community Reference Laboratory for Salmonella (CRL-Salmonella, Bilthoven, The Netherlands) in collaboration with the Public Health Laboratory Services (PHLS), London, UK. Seventeen National Reference Laboratories for Salmonella (NRLs-Salmonella) and 15 EnterNet laboratories (ENLs) participated in the study. Three of the NRLs for Salmonella are also ENLs. The results of these three NRL-ENL laboratories will only be evaluated with the NRLs for Salmonella. In total, 19 strains of the species Salmonella enterica subsp. enterica and one strain of the species Salmonella enterica subsp. arizonae were selected for serotyping and antimicrobial susceptibility testing, while 10 strains of Salmonella Typhimurium (STM) and 10 strains of Salmonella Enteritidis (SE) were selected for phage typing. In general, no problems were encountered with the typing of the O antigens. However, some laboratories had problems with typing the H antigens. Antimicrobial susceptibility testing revealed data showing that standardisation of this technique would be required to allow for comparison between laboratories. The majority of the EnterNet Laboratories and National Reference Laboratories for Salmonella did not encounter major problems with phage typing of STM and SE strains.