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dc.contributor.authorBaggen, Jim
dc.contributor.authorHurdiss, Daniel L
dc.contributor.authorZocher, Georg
dc.contributor.authorMistry, Nitesh
dc.contributor.authorRoberts, Richard W
dc.contributor.authorSlager, Jasper J
dc.contributor.authorGuo, Hongbo
dc.contributor.authorvan Vliet, Arno L W
dc.contributor.authorWahedi, Maryam
dc.contributor.authorBenschop, Kimberley
dc.contributor.authorDuizer, Erwin
dc.contributor.authorde Haan, Cornelis A M
dc.contributor.authorde Vries, Erik
dc.contributor.authorCasasnovas, José M
dc.contributor.authorde Groot, Raoul J
dc.contributor.authorArnberg, Niklas
dc.contributor.authorStehle, Thilo
dc.contributor.authorRanson, Neil A
dc.contributor.authorThibaut, Hendrik Jan
dc.contributor.authorvan Kuppeveld, Frank J M
dc.date.accessioned2018-01-05T09:07:24Z
dc.date.available2018-01-05T09:07:24Z
dc.date.issued2017-12-28
dc.identifier.citationRole of enhanced receptor engagement in the evolution of a pandemic acute hemorrhagic conjunctivitis virus. 2017 Proc. Natl. Acad. Sci. U.S.A.en
dc.identifier.issn1091-6490
dc.identifier.pmid29284752
dc.identifier.doi10.1073/pnas.1713284115
dc.identifier.urihttp://hdl.handle.net/10029/621037
dc.description.abstractAcute hemorrhagic conjunctivitis (AHC) is a painful, contagious eye disease, with millions of cases in the last decades. Coxsackievirus A24 (CV-A24) was not originally associated with human disease, but in 1970 a pathogenic "variant" (CV-A24v) emerged, which is now the main cause of AHC. Initially, this variant circulated only in Southeast Asia, but it later spread worldwide, accounting for numerous AHC outbreaks and two pandemics. While both CV-A24 variant and nonvariant strains still circulate in humans, only variant strains cause AHC for reasons that are yet unknown. Since receptors are important determinants of viral tropism, we set out to map the CV-A24 receptor repertoire and establish whether changes in receptor preference have led to the increased pathogenicity and rapid spread of CV-A24v. Here, we identify ICAM-1 as an essential receptor for both AHC-causing and non-AHC strains. We provide a high-resolution cryo-EM structure of a virus-ICAM-1 complex, which revealed critical ICAM-1-binding residues. These data could help identify a possible conserved mode of receptor engagement among ICAM-1-binding enteroviruses and rhinoviruses. Moreover, we identify a single capsid substitution that has been adopted by all pandemic CV-A24v strains and we reveal that this adaptation enhances the capacity of CV-A24v to bind sialic acid. Our data elucidate the CV-A24v receptor repertoire and point to a role of enhanced receptor engagement in the adaptation to the eye, possibly enabling pandemic spread.
dc.language.isoenen
dc.rightsArchived with thanks to Proceedings of the National Academy of Sciences of the United States of Americaen
dc.titleRole of enhanced receptor engagement in the evolution of a pandemic acute hemorrhagic conjunctivitis virus.en
dc.identifier.journalProc Natl Acad Sci U.S.A. 2018; advance online publication (ahead of print)en
html.description.abstractAcute hemorrhagic conjunctivitis (AHC) is a painful, contagious eye disease, with millions of cases in the last decades. Coxsackievirus A24 (CV-A24) was not originally associated with human disease, but in 1970 a pathogenic "variant" (CV-A24v) emerged, which is now the main cause of AHC. Initially, this variant circulated only in Southeast Asia, but it later spread worldwide, accounting for numerous AHC outbreaks and two pandemics. While both CV-A24 variant and nonvariant strains still circulate in humans, only variant strains cause AHC for reasons that are yet unknown. Since receptors are important determinants of viral tropism, we set out to map the CV-A24 receptor repertoire and establish whether changes in receptor preference have led to the increased pathogenicity and rapid spread of CV-A24v. Here, we identify ICAM-1 as an essential receptor for both AHC-causing and non-AHC strains. We provide a high-resolution cryo-EM structure of a virus-ICAM-1 complex, which revealed critical ICAM-1-binding residues. These data could help identify a possible conserved mode of receptor engagement among ICAM-1-binding enteroviruses and rhinoviruses. Moreover, we identify a single capsid substitution that has been adopted by all pandemic CV-A24v strains and we reveal that this adaptation enhances the capacity of CV-A24v to bind sialic acid. Our data elucidate the CV-A24v receptor repertoire and point to a role of enhanced receptor engagement in the adaptation to the eye, possibly enabling pandemic spread.


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