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dc.contributor.authorEderveen, Thomas H A
dc.contributor.authorFerwerda, Gerben
dc.contributor.authorAhout, Inge M
dc.contributor.authorVissers, Marloes
dc.contributor.authorde Groot, Ronald
dc.contributor.authorBoekhorst, Jos
dc.contributor.authorTimmerman, Harro M
dc.contributor.authorHuynen, Martijn A
dc.contributor.authorvan Hijum, Sacha A F T
dc.contributor.authorde Jonge, Marien I
dc.date.accessioned2018-01-16T09:16:56Z
dc.date.available2018-01-16T09:16:56Z
dc.date.issued2018-01-11
dc.identifier.citationHaemophilus is overrepresented in the nasopharynx of infants hospitalized with RSV infection and associated with increased viral load and enhanced mucosal CXCL8 responses. 2018, 6 (1):10 Microbiomeen
dc.identifier.issn2049-2618
dc.identifier.pmid29325581
dc.identifier.doi10.1186/s40168-017-0395-y
dc.identifier.urihttp://hdl.handle.net/10029/621158
dc.description.abstractWhile almost all infants are infected with respiratory syncytial virus (RSV) before the age of 2 years, only a small percentage develops severe disease. Previous studies suggest that the nasopharyngeal microbiome affects disease development. We therefore studied the effect of the nasopharyngeal microbiome on viral load and mucosal cytokine responses, two important factors influencing the pathophysiology of RSV disease. To determine the relation between (i) the microbiome of the upper respiratory tract, (ii) viral load, and (iii) host mucosal inflammation during an RSV infection, nasopharyngeal microbiota profiles of RSV infected infants (< 6 months) with different levels of disease severity and age-matched healthy controls were determined by 16S rRNA marker gene sequencing. The viral load was measured using qPCR. Nasopharyngeal CCL5, CXCL10, MMP9, IL6, and CXCL8 levels were determined with ELISA.
dc.language.isoenen
dc.rightsArchived with thanks to Microbiomeen
dc.titleHaemophilus is overrepresented in the nasopharynx of infants hospitalized with RSV infection and associated with increased viral load and enhanced mucosal CXCL8 responses.en
dc.typeArticleen
dc.identifier.journalMicrobiome 2018; 6(1):10en
html.description.abstractWhile almost all infants are infected with respiratory syncytial virus (RSV) before the age of 2 years, only a small percentage develops severe disease. Previous studies suggest that the nasopharyngeal microbiome affects disease development. We therefore studied the effect of the nasopharyngeal microbiome on viral load and mucosal cytokine responses, two important factors influencing the pathophysiology of RSV disease. To determine the relation between (i) the microbiome of the upper respiratory tract, (ii) viral load, and (iii) host mucosal inflammation during an RSV infection, nasopharyngeal microbiota profiles of RSV infected infants (< 6 months) with different levels of disease severity and age-matched healthy controls were determined by 16S rRNA marker gene sequencing. The viral load was measured using qPCR. Nasopharyngeal CCL5, CXCL10, MMP9, IL6, and CXCL8 levels were determined with ELISA.


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