Detection and dissemination of Toxoplasma gondii in experimentally infected calves, a single test does not tell the whole story.
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Bartley, Paul M
van der Giessen, Joke
Innes, Elisabeth A
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TitleDetection and dissemination of Toxoplasma gondii in experimentally infected calves, a single test does not tell the whole story.
Published inParasit Vectors 2018; 11(1):45
PubliekssamenvattingAlthough the detection of Toxoplasma gondii in bovine tissues is rare, beef might be an important source of human infection. The use of molecular techniques, such as magnetic capture qPCR (MC-qPCR), in combination with the gold standard method for isolating the parasite (mouse bioassay), may increase the sensitivity of T. gondii detection in infected cattle. The risk of transmission of the parasite to humans from undercooked/raw beef is not fully known and further knowledge about the predilection sites of T. gondii within cattle is needed. In the current study, six Holstein Friesian calves (Bos taurus) were experimentally infected with 106 T. gondii oocysts of the M4 strain and, following euthanasia (42 dpi), pooled tissues were tested for presence of the parasite by mouse bioassay and MC-qPCR.
- The relationship between the presence of antibodies and direct detection of Toxoplasma gondii in slaughtered calves and cattle in four European countries.
- Authors: Opsteegh M, Spano F, Aubert D, Balea A, Burrells A, Cherchi S, Cornelissen JBWJ, Dam-Deisz C, Guitian J, Györke A, Innes EA, Katzer F, Limon G, Possenti A, Pozio E, Schares G, Villena I, Wisselink HJ, van der Giessen JWB
- Issue date: 2019 Jun
- Toxoplasma gondii: detection by mouse bioassay, histopathology, and polymerase chain reaction in tissues from experimentally infected pigs.
- Authors: Garcia JL, Gennari SM, Machado RZ, Navarro IT
- Issue date: 2006 Aug
- Toxoplasma gondii infections in chickens - performance of various antibody detection techniques in serum and meat juice relative to bioassay and DNA detection methods.
- Authors: Schares G, Koethe M, Bangoura B, Geuthner AC, Randau F, Ludewig M, Maksimov P, Sens M, Bärwald A, Conraths FJ, Villena I, Aubert D, Opsteegh M, Van der Giessen J
- Issue date: 2018 Aug
- Predilection sites for Toxoplasma gondii in sheep tissues revealed by magnetic capture and real-time PCR detection.
- Authors: Juránková J, Basso W, Neumayerová H, Frencová A, Baláž V, Deplazes P, Koudela B
- Issue date: 2015 Dec
- Distribution of cysts and tachyzoites in calves and pregnant cows inoculated with Toxoplasma gondii oocysts.
- Authors: Dubey JP
- Issue date: 1983 Oct