Detection and dissemination of Toxoplasma gondii in experimentally infected calves, a single test does not tell the whole story.
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Authors
Burrells, AlisonTaroda, Alessandra
Opsteegh, Marieke
Schares, Gereon
Benavides, Julio
Dam-Deisz, Cecile
Bartley, Paul M
Chianini, Francesca
Villena, Isabella
van der Giessen, Joke
Innes, Elisabeth A
Katzer, Frank
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ArticleLanguage
en
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Detection and dissemination of Toxoplasma gondii in experimentally infected calves, a single test does not tell the whole story.Published in
Parasit Vectors 2018; 11(1):45Publiekssamenvatting
Although the detection of Toxoplasma gondii in bovine tissues is rare, beef might be an important source of human infection. The use of molecular techniques, such as magnetic capture qPCR (MC-qPCR), in combination with the gold standard method for isolating the parasite (mouse bioassay), may increase the sensitivity of T. gondii detection in infected cattle. The risk of transmission of the parasite to humans from undercooked/raw beef is not fully known and further knowledge about the predilection sites of T. gondii within cattle is needed. In the current study, six Holstein Friesian calves (Bos taurus) were experimentally infected with 106 T. gondii oocysts of the M4 strain and, following euthanasia (42 dpi), pooled tissues were tested for presence of the parasite by mouse bioassay and MC-qPCR.PMID
29347971ae974a485f413a2113503eed53cd6c53
10.1186/s13071-018-2632-z
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