Brucella Pinnipedialis in grey seals (Halichoerus grypus) and harbor seals (Phoca vitulina) in the Netherlands
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
AuthorsKroese, Michiel V
Bisselink, Yvette J W M
van Tulden, Peter W
Koene, Miriam G J
Roest, Hendrik I J
Ruuls, Robin C
Backer, Jantien A
van der Giessen, Joke W B
Willemsen, Peter T J
MetadataShow full item record
TitleBrucella Pinnipedialis in grey seals (Halichoerus grypus) and harbor seals (Phoca vitulina) in the Netherlands
Published inJ Wildlife Dis 2018; 54|(3):439-49
PubliekssamenvattingBrucellosis is a zoonotic disease with terrestrial or marine wildlife animals as potential reservoirs for the disease in livestock and human populations. The primary aim of this study was to assess the presence of Brucella pinnipedialis in marine mammals living along the Dutch coast and to observe a possible correlation between the presence of B. pinnipedialis and accompanying pathology found in infected animals. The overall prevalence of Brucella spp. antibodies in sera from healthy wild grey seals ( Halichoerus grypus; n=11) and harbor seals ( Phoca vitulina; n=40), collected between 2007 and 2013 ranged from 25% to 43%. Additionally, tissue samples of harbor seals collected along the Dutch shores between 2009 and 2012, were tested for the presence of Brucella spp. In total, 77% (30/39) seals were found to be positive for Brucella by IS 711 real-time PCR in one or more tissue samples, including pulmonary nematodes. Viable Brucella was cultured from 40% (12/30) real-time PCR-positive seals, and was isolated from liver, lung, pulmonary lymph node, pulmonary nematode, or spleen, but not from any PCR-negative seals. Tissue samples from lung and pulmonary lymph nodes were the main source of viable Brucella bacteria. All isolates were typed as B. pinnipedialis by multiple-locus variable number of tandem repeats analysis-16 clustering and matrix-assisted laser desorption ionization-time of flight mass spectrometry, and of sequence type ST25 by multilocus sequence typing analysis. No correlation was observed between Brucella infection and pathology. This report displays the isolation and identification of B. pinnipedialis in marine mammals in the Dutch part of the Atlantic Ocean.
- Brucella pinnipedialis infections in Pacific harbor seals (Phoca vitulina richardsi) from Washington State, USA.
- Authors: Lambourn DM, Garner M, Ewalt D, Raverty S, Sidor I, Jeffries SJ, Rhyan J, Gaydos JK
- Issue date: 2013 Oct
- Isolation of Brucella pinnipedialis from Grey Seals (Halichoerus grypus) in the Baltic Sea.
- Authors: Hirvelä-Koski V, Nylund M, Skrzypczak T, Heikkinen P, Kauhala K, Jay M, Isomursu M
- Issue date: 2017 Oct
- Brucella pinnipedialis in lungworms Parafilaroides sp. and Pacific harbor seals Phoca vitulina richardsi: proposed pathogenesis.
- Authors: Rhyan J, Garner M, Spraker T, Lambourn D, Cheville N
- Issue date: 2018 Nov 6
- Prevalence of Brucella pinnipediae in healthy hooded seals (Cystophora cristata) from the North Atlantic Ocean and ringed seals (Phoca hispida) from Svalbard.
- Authors: Tryland M, Sørensen KK, Godfroid J
- Issue date: 2005 Jan 31
- First isolation of Brucella pinnipedialis and detection of Brucella antibodies from bearded seals Erignathus barbatus.
- Authors: Foster G, Nymo IH, Kovacs KM, Beckmen KB, Brownlow AC, Baily JL, Dagleish MP, Muchowski J, Perrett LL, Tryland M, Lydersen C, Godfroid J, McGovern B, Whatmore AM
- Issue date: 2018 Mar 22