Salmonella enterica Prophage Sequence Profiles Reflect Genome Diversity and Can Be Used for High Discrimination Subtyping.
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Authors
Mottawea, WalidDuceppe, Marc-Olivier
Dupras, Andrée A
Usongo, Valentine
Jeukens, Julie
Freschi, Luca
Emond-Rheault, Jean-Guillaume
Hamel, Jeremie
Kukavica-Ibrulj, Irena
Boyle, Brian
Gill, Alexander
Burnett, Elton
Franz, Eelco
Arya, Gitanjali
Weadge, Joel T
Gruenheid, Samantha
Wiedmann, Martin
Huang, Hongsheng
Daigle, France
Moineau, Sylvain
Bekal, Sadjia
Levesque, Roger C
Goodridge, Lawrence D
Ogunremi, Dele
Type
ArticleLanguage
en
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Show full item recordTitle
Salmonella enterica Prophage Sequence Profiles Reflect Genome Diversity and Can Be Used for High Discrimination Subtyping.Published in
Front Microbiol 2018; 9:836Publiekssamenvatting
Non-typhoidal Salmonella is a leading cause of foodborne illness worldwide. Prompt and accurate identification of the sources of Salmonella responsible for disease outbreaks is crucial to minimize infections and eliminate ongoing sources of contamination. Current subtyping tools including single nucleotide polymorphism (SNP) typing may be inadequate, in some instances, to provide the required discrimination among epidemiologically unrelated Salmonella strains. Prophage genes represent the majority of the accessory genes in bacteria genomes and have potential to be used as high discrimination markers in Salmonella. In this study, the prophage sequence diversity in different Salmonella serovars and genetically related strains was investigated. Using whole genome sequences of 1,760 isolates of S. enterica representing 151 Salmonella serovars and 66 closely related bacteria, prophage sequences were identified from assembled contigs using PHASTER. We detected 154 different prophages in S. enterica genomes. Prophage sequences were highly variable among S. enterica serovars with a median ± interquartile range (IQR) of 5 ± 3 prophage regions per genome. While some prophage sequences were highly conserved among the strains of specific serovars, few regions were lineage specific. Therefore, strains belonging to each serovar could be clustered separately based on their prophage content. Analysis of S. Enteritidis isolates from seven outbreaks generated distinct prophage profiles for each outbreak. Taken altogether, the diversity of the prophage sequences correlates with genome diversity. Prophage repertoires provide an additional marker for differentiating S. enterica subtypes during foodborne outbreaks.PMID
29780368ae974a485f413a2113503eed53cd6c53
10.3389/fmicb.2018.00836
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