Salmonella enterica Prophage Sequence Profiles Reflect Genome Diversity and Can Be Used for High Discrimination Subtyping.
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
Dupras, Andrée A
Weadge, Joel T
Levesque, Roger C
Goodridge, Lawrence D
MetadataShow full item record
TitleSalmonella enterica Prophage Sequence Profiles Reflect Genome Diversity and Can Be Used for High Discrimination Subtyping.
Published inFront Microbiol 2018; 9:836
PubliekssamenvattingNon-typhoidal Salmonella is a leading cause of foodborne illness worldwide. Prompt and accurate identification of the sources of Salmonella responsible for disease outbreaks is crucial to minimize infections and eliminate ongoing sources of contamination. Current subtyping tools including single nucleotide polymorphism (SNP) typing may be inadequate, in some instances, to provide the required discrimination among epidemiologically unrelated Salmonella strains. Prophage genes represent the majority of the accessory genes in bacteria genomes and have potential to be used as high discrimination markers in Salmonella. In this study, the prophage sequence diversity in different Salmonella serovars and genetically related strains was investigated. Using whole genome sequences of 1,760 isolates of S. enterica representing 151 Salmonella serovars and 66 closely related bacteria, prophage sequences were identified from assembled contigs using PHASTER. We detected 154 different prophages in S. enterica genomes. Prophage sequences were highly variable among S. enterica serovars with a median ± interquartile range (IQR) of 5 ± 3 prophage regions per genome. While some prophage sequences were highly conserved among the strains of specific serovars, few regions were lineage specific. Therefore, strains belonging to each serovar could be clustered separately based on their prophage content. Analysis of S. Enteritidis isolates from seven outbreaks generated distinct prophage profiles for each outbreak. Taken altogether, the diversity of the prophage sequences correlates with genome diversity. Prophage repertoires provide an additional marker for differentiating S. enterica subtypes during foodborne outbreaks.
- Prophage Integrase Typing Is a Useful Indicator of Genomic Diversity in <i>Salmonella enterica</i>.
- Authors: Colavecchio A, D'Souza Y, Tompkins E, Jeukens J, Freschi L, Emond-Rheault JG, Kukavica-Ibrulj I, Boyle B, Bekal S, Tamber S, Levesque RC, Goodridge LD
- Issue date: 2017
- A novel prophage identified in strains from Salmonella enterica serovar Enteritidis is a phylogenetic signature of the lineage ST-1974.
- Authors: D'Alessandro B, Pérez Escanda V, Balestrazzi L, Iriarte A, Pickard D, Yim L, Chabalgoity JA, Betancor L
- Issue date: 2018 Mar
- Evaluation of WGS-subtyping methods for epidemiological surveillance of foodborne salmonellosis.
- Authors: Mohammed M, Thapa S
- Issue date: 2020
- Comparison of advanced whole genome sequence-based methods to distinguish strains of Salmonella enterica serovar Heidelberg involved in foodborne outbreaks in Québec.
- Authors: Vincent C, Usongo V, Berry C, Tremblay DM, Moineau S, Yousfi K, Doualla-Bell F, Fournier E, Nadon C, Goodridge L, Bekal S
- Issue date: 2018 Aug
- Comparative genomic analysis of 142 bacteriophages infecting Salmonella enterica subsp. enterica.
- Authors: Gao R, Naushad S, Moineau S, Levesque R, Goodridge L, Ogunremi D
- Issue date: 2020 May 26