• Login
    View Item 
    •   Home
    • Articles and other publications by RIVM employees
    • Miscellaneous
    • View Item
    •   Home
    • Articles and other publications by RIVM employees
    • Miscellaneous
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    RIVM Publications RepositoryCommunitiesTitleAuthorsIssue DateSubmit Date

    My Account

    LoginRegister

    Statistics

    Display statistics

    Distinguishing Echinococcus granulosus sensu stricto genotypes G1 and G3 with confidence: A practical guide.

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Thumbnail
    Name:
    Publisher version
    View Source
    Access full-text PDFOpen Access
    View Source
    Check access options
    Check access options
    Average rating
     
       votes
    Cast your vote
    You can rate an item by clicking the amount of stars they wish to award to this item. When enough users have cast their vote on this item, the average rating will also be shown.
    Star rating
     
    Your vote was cast
    Thank you for your feedback
    Authors
    Kinkar, Liina
    Laurimäe, Teivi
    Acosta-Jamett, Gerardo
    Andresiuk, Vanessa
    Balkaya, Ibrahim
    Casulli, Adriano
    Gasser, Robin B
    González, Luis Miguel
    Haag, Karen L
    Zait, Houria
    Irshadullah, Malik
    Jabbar, Abdul
    Jenkins, David J
    Manfredi, Maria Teresa
    Mirhendi, Hossein
    M'rad, Selim
    Rostami-Nejad, Mohammad
    Oudni-M'rad, Myriam
    Pierangeli, Nora Beatriz
    Ponce-Gordo, Francisco
    Rehbein, Steffen
    Sharbatkhori, Mitra
    Kia, Eshrat Beigom
    Simsek, Sami
    Soriano, Silvia Viviana
    Sprong, Hein
    Šnábel, Viliam
    Umhang, Gérald
    Varcasia, Antonio
    Saarma, Urmas
    Show allShow less
    Type
    Article
    Language
    en
    
    Metadata
    Show full item record
    Title
    Distinguishing Echinococcus granulosus sensu stricto genotypes G1 and G3 with confidence: A practical guide.
    Published in
    Infect Genet Evol 2018; 64:178-84
    Publiekssamenvatting
    Cystic echinococcosis (CE), a zoonotic disease caused by tapeworms of the species complex Echinococcus granulosus sensu lato, represents a substantial global health and economic burden. Within this complex, E. granulosus sensu stricto (genotypes G1 and G3) is the most frequent causative agent of human CE. Currently, there is no fully reliable method for assigning samples to genotypes G1 and G3, as the commonly used mitochondrial cox1 and nad1 genes are not sufficiently consistent for the identification and differentiation of these genotypes. Thus, a new genetic assay is required for the accurate assignment of G1 and G3. Here we use a large dataset of near-complete mtDNA sequences (n = 303) to reveal the extent of genetic variation of G1 and G3 on a broad geographical scale and to identify reliable informative positions for G1 and G3. Based on extensive sampling and sequencing data, we developed a new method, that is simple and cost-effective, to designate samples to genotypes G1 and G3. We found that the nad5 is the best gene in mtDNA to differentiate between G1 and G3, and developed new primers for the analysis. Our results also highlight problems related to the commonly used cox1 and nad1. To guarantee consistent identification of G1 and G3, we suggest using the sequencing of the nad5 gene region (680 bp). This region contains six informative positions within a relatively short fragment of the mtDNA, allowing the differentiation of G1 and G3 with confidence. Our method offers clear advantages over the previous ones, providing a significantly more consistent means to distinguish G1 and G3 than the commonly used cox1 and nad1.
    DOI
    10.1016/j.meegid.2018.06.026
    PMID
    29936039
    URI
    http://hdl.handle.net/10029/622032
    ae974a485f413a2113503eed53cd6c53
    10.1016/j.meegid.2018.06.026
    Scopus Count
    Collections
    Miscellaneous

    entitlement

    Related articles

    • New mitogenome and nuclear evidence on the phylogeny and taxonomy of the highly zoonotic tapeworm Echinococcus granulosus sensu stricto.
    • Authors: Kinkar L, Laurimäe T, Sharbatkhori M, Mirhendi H, Kia EB, Ponce-Gordo F, Andresiuk V, Simsek S, Lavikainen A, Irshadullah M, Umhang G, Oudni-M'rad M, Acosta-Jamett G, Rehbein S, Saarma U
    • Issue date: 2017 Aug
    • Echinococcus granulosus sensu stricto G1 is the predominant genotype in human and livestock isolates from Turkey and Iran, based on mitochondrial nad5 gene differentiation.
    • Authors: Shahabi S, Sarkari B, Barazesh A
    • Issue date: 2021 Jul 20
    • Genetic diversity and phylogeography of the elusive, but epidemiologically important Echinococcus granulosus sensu stricto genotype G3.
    • Authors: Kinkar L, Laurimäe T, Balkaya I, Casulli A, Zait H, Irshadullah M, Sharbatkhori M, Mirhendi H, Rostami-Nejad M, Ponce-Gordo F, Rehbein S, Kia EB, Simsek S, Šnábel V, Umhang G, Varcasia A, Saarma U
    • Issue date: 2018 Oct
    • A novel zoonotic genotype related to Echinococcus granulosus sensu stricto from southern Ethiopia.
    • Authors: Wassermann M, Woldeyes D, Gerbi BM, Ebi D, Zeyhle E, Mackenstedt U, Petros B, Tilahun G, Kern P, Romig T
    • Issue date: 2016 Sep
    • Echinococcus granulosus (sensu stricto) (G1, G3) and E. ortleppi (G5) in Pakistan: phylogeny, genetic diversity and population structural analysis based on mitochondrial DNA.
    • Authors: Alvi MA, Ohiolei JA, Saqib M, Li L, Tayyab MH, Alvi AA, Wu YT, Fu BQ, Yan HB, Jia WZ
    • Issue date: 2020 Jul 13

    DSpace software (copyright © 2002 - 2023)  DuraSpace
    Quick Guide | Contact Us
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.