Gene profiling-based phenotyping for identification of cellular parameters that contribute to fitness, stress-tolerance and virulence of Listeria monocytogenes variants.
Name:
Publisher version
View Source
Access full-text PDFOpen Access
View Source
Check access options
Check access options
Average rating
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Star rating
Your vote was cast
Thank you for your feedback
Thank you for your feedback
Authors
Koomen, Jeroenden Besten, Heidy M W
Metselaar, Karin I
Tempelaars, Marcel H
Wijnands, Lucas M
Zwietering, Marcel H
Abee, Tjakko
Type
ArticleLanguage
en
Metadata
Show full item recordTitle
Gene profiling-based phenotyping for identification of cellular parameters that contribute to fitness, stress-tolerance and virulence of Listeria monocytogenes variants.Published in
Int J Food Microbiol 2018; 283:14-21Publiekssamenvatting
Microbial population heterogeneity allows for a differential microbial response to environmental stresses and can lead to the selection of stress resistant variants. In this study, we have used two different stress resistant variants of Listeria monocytogenes LO28 with mutations in the rpsU gene encoding ribosomal protein S21, to elucidate features that can contribute to fitness, stress-tolerance and host interaction using a comparative gene profiling and phenotyping approach. Transcriptome analysis showed that 116 genes were upregulated and 114 genes were downregulated in both rpsU variants. Upregulated genes included a major contribution of SigB-controlled genes such as intracellular acid resistance-associated glutamate decarboxylase (GAD) (gad3), genes involved in compatible solute uptake (opuC), glycerol metabolism (glpF, glpK, glpD), and virulence (inlA, inlB). Downregulated genes in the two variants involved mainly genes involved in flagella synthesis and motility. Phenotyping results of the two rpsU variants matched the gene profiling data including enhanced freezing resistance conceivably linked to compatible solute accumulation, higher glycerol utilisation rates, and better adhesion to Caco 2 cells presumably linked to higher expression of internalins. Also, bright field and electron microscopy analysis confirmed reduced flagellation of the variants. The activation of SigB-mediated stress defence offers an explanation for the multiple-stress resistant phenotype in rpsU variants.PMID
29935377ae974a485f413a2113503eed53cd6c53
10.1016/j.ijfoodmicro.2018.06.003
Scopus Count
Collections
Related articles
- Amino acid substitutions in ribosomal protein RpsU enable switching between high fitness and multiple-stress resistance in Listeria monocytogenes.
- Authors: Koomen J, Huijboom L, Ma X, Tempelaars MH, Boeren S, Zwietering MH, den Besten HMW, Abee T
- Issue date: 2021 Aug 2
- Cold-Shock Domain Family Proteins (Csps) Are Involved in Regulation of Virulence, Cellular Aggregation, and Flagella-Based Motility in Listeria monocytogenes.
- Authors: Eshwar AK, Guldimann C, Oevermann A, Tasara T
- Issue date: 2017
- [Contribution of sigma B to environmental stress tolerance in Listeria monocytogenes--a review].
- Authors: Zhang Q, Feng Y, Zhou Q, Luo Q, Zhang X, Qin L
- Issue date: 2009 Oct
- Thioredoxin A Is Essential for Motility and Contributes to Host Infection of Listeria monocytogenes via Redox Interactions.
- Authors: Cheng C, Dong Z, Han X, Wang H, Jiang L, Sun J, Yang Y, Ma T, Shao C, Wang X, Chen Z, Fang W, Freitag NE, Huang H, Song H
- Issue date: 2017
- Contributions of a LysR Transcriptional Regulator to Listeria monocytogenes Virulence and Identification of Its Regulons.
- Authors: Abdelhamed H, Ramachandran R, Narayanan L, Ozdemir O, Cooper A, Olivier AK, Karsi A, Lawrence ML
- Issue date: 2020 Apr 27