• Login
    View Item 
    •   Home
    • Articles and other publications by RIVM employees
    • Miscellaneous
    • View Item
    •   Home
    • Articles and other publications by RIVM employees
    • Miscellaneous
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of WARPCommunitiesTitleAuthorsIssue DateSubmit DateSubjectsPublisherDepartmentThis CollectionTitleAuthorsIssue DateSubmit DateSubjectsPublisherDepartment

    My Account

    LoginRegister

    Statistics

    Display statistics

    Simulation of mouse and rat spermatogenesis to inform genotoxicity testing using OECD test guideline 488.

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Thumbnail
    Name:
    Publisher version
    View Source
    Access full-text PDFOpen Access
    View Source
    Check access options
    Check access options
    Average rating
     
       votes
    Cast your vote
    You can rate an item by clicking the amount of stars they wish to award to this item. When enough users have cast their vote on this item, the average rating will also be shown.
    Star rating
     
    Your vote was cast
    Thank you for your feedback
    Authors
    Marchetti, Francesco
    Aardema, Marilyn
    Beevers, Carol
    van Benthem, Jan
    Douglas, George R
    Godschalk, Roger
    Yauk, Carole L
    Young, Robert
    Williams, Andrew
    Type
    Article
    Language
    en
    
    Metadata
    Show full item record
    Title
    Simulation of mouse and rat spermatogenesis to inform genotoxicity testing using OECD test guideline 488.
    Published in
    Mutat Res 2018; 832-833:19-28
    Publiekssamenvatting
    The Organisation for Economic Co-operation and Development Test Guideline (TG) 488 for the transgenic rodent (TGR) mutation assay recommends two sampling times for assessing germ cell mutagenicity following the required 28-day exposure period: 28 + > 49 days for mouse sperm and 28 + >70 days for rat sperm from the cauda epididymis, or three days (i.e., 28 + 3d) for germ cells from seminiferous tubules (hereafter, tubule germ cells) plus caudal sperm for mouse and rat. Although the latter protocol is commonly used for mutagenicity testing in somatic tissues, it has several shortcomings for germ cell testing because it provides limited exposure of the proliferating phase of spermatogenesis when mutations are fixed in the transgene. Indeed, analysis of sperm at 28 + 3d has generated negative results with established germ cell mutagens, while the analysis of tubule germ cells has generated both positive and either negative or equivocal results. The Germ Cell workgroup of the Genetic Toxicology Technical Committee of the Health and Environmental Sciences Institute modelled mouse and rat spermatogenesis to better define the exposure history of the cell population collected from seminiferous tubules. The modelling showed that mouse tubule germ cells at 28 + 3d receive, as a whole, 42% of the total exposure during the proliferating phase. This percentage increases to 99% at 28 + 28d and reaches 100% at 28 + 30d. In the rat, these percentages are 22% and 80% at 28 + 3d and 28 + 28d, reaching 100% at 28 + 44d. These results show that analysis of tubule germ cells at 28 + 28d may be an effective protocol for assessing germ cell mutagenicity in mice and rats using TG 488. Since TG 488 recommends the 28 + 28d protocol for slow dividing somatic tissues, this appears to be a better compromise than 28 + 3d when slow dividing somatic tissues or germ cells are the critical tissues of interest.
    DOI
    10.1016/j.mrgentox.2018.05.020
    PMID
    30057017
    URI
    http://hdl.handle.net/10029/622125
    ae974a485f413a2113503eed53cd6c53
    10.1016/j.mrgentox.2018.05.020
    Scopus Count
    Collections
    Miscellaneous

    entitlement

    Related articles

    • Identifying germ cell mutagens using OECD test guideline 488 (transgenic rodent somatic and germ cell gene mutation assays) and integration with somatic cell testing.
    • Authors: Marchetti F, Aardema MJ, Beevers C, van Benthem J, Godschalk R, Williams A, Yauk CL, Young R, Douglas GR
    • Issue date: 2018 Aug
    • Benzo(a)pyrene Is Mutagenic in Mouse Spermatogonial Stem Cells and Dividing Spermatogonia.
    • Authors: O'Brien JM, Beal MA, Yauk CL, Marchetti F
    • Issue date: 2016 Aug
    • Integration of sperm DNA damage assessment into OECD test guidelines for genotoxicity testing using the MutaMouse model.
    • Authors: Maurice C, O'Brien JM, Yauk CL, Marchetti F
    • Issue date: 2018 Oct 15
    • In vivo genotoxicity testing strategies: Report from the 7th International workshop on genotoxicity testing (IWGT).
    • Authors: Kirkland D, Uno Y, Luijten M, Beevers C, van Benthem J, Burlinson B, Dertinger S, Douglas GR, Hamada S, Horibata K, Lovell DP, Manjanatha M, Martus HJ, Mei N, Morita T, Ohyama W, Williams A
    • Issue date: 2019 Nov
    • Spermatogenesis by Sisyphus: proliferating stem germ cells fail to repopulate the testis after 'irreversible' injury.
    • Authors: Boekelheide K, Schoenfeld HA
    • Issue date: 2001

    DSpace software (copyright © 2002 - 2021)  DuraSpace
    Quick Guide | Contact Us
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.