Detection of opsonizing antibodies directed against a recently circulating Bordetella pertussis strain in paired plasma samples from symptomatic and recovered pertussis patients.
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
AuthorsHovingh, Elise S
Bonačić Marinović, Axel A
Jan Hamstra, Hendrik
Mughini Gras, Lapo
van Twillert, Inonge
van Els, Cecile A C M
MetadataShow full item record
TitleDetection of opsonizing antibodies directed against a recently circulating Bordetella pertussis strain in paired plasma samples from symptomatic and recovered pertussis patients.
Published inSci Rep 2018; 8(1):12039
PubliekssamenvattingCorrelates of protection (CoPs) against the highly contagious respiratory disease whooping cough, caused by Bordetella pertussis, remain elusive. Characterizing the antibody response to this pathogen is essential towards identifying potential CoPs. Here, we evaluate levels, avidity and functionality of B. pertussis-specific-antibodies from paired plasma samples derived from symptomatic and recovered pertussis patients, as well as controls. Natural infection is expected to induce protective immunity. IgG levels and avidity to nine B. pertussis antigens were determined using a novel multiplex panel. Furthermore, opsonophagocytosis of a B. pertussis clinical isolate by neutrophils was measured. Findings indicate that following infection, B. pertussis-specific antibody levels of (ex-) pertussis patients waned, while the avidity of antibodies directed against the majority of studied antigens increased. Opsonophagocytosis indices decreased upon recovery, but remained higher than controls. Random forest analysis of all the data revealed that 28% of the opsonophagocytosis index variances could be explained by filamentous hemagglutinin- followed by pertussis toxin-specific antibodies. We propose to further explore which other B. pertussis-specific antibodies can better predict opsonophagocytosis. Moreover, other B. pertussis-specific antibody functions as well as the possible integration of these functions in combination with other immune cell properties should be evaluated towards the identification of CoPs against pertussis.
The following license files are associated with this item:
- Creative Commons
Except where otherwise noted, this item's license is described as Archived with thanks to Scientific reports
- [Serum immunoglobulin IgG subclass distribution of antibody responses to pertussis toxin and filamentous hemagglutinin of Bordetella pertussis in patients with whooping cough].
- Authors: Rastawicki W, Smietańska K, Rokosz-Chudziak N, Jagielski M
- Issue date: 2013
- Crucial role of antibodies to pertactin in Bordetella pertussis immunity.
- Authors: Hellwig SM, Rodriguez ME, Berbers GA, van de Winkel JG, Mooi FR
- Issue date: 2003 Sep 1
- Suppression of serum antibody responses by pertussis toxin after respiratory tract colonization by Bordetella pertussis and identification of an immunodominant lipoprotein.
- Authors: Carbonetti NH, Artamonova GV, Andreasen C, Dudley E, Mays RM, Worthington ZE
- Issue date: 2004 Jun
- Serological correlates in whooping cough.
- Authors: Granström M, Granström G
- Issue date: 1993
- Differences in avidity of IgG antibodies to pertussis toxin after acellular pertussis booster vaccination and natural infection.
- Authors: Barkoff AM, Gröndahl-Yli-Hannuksela K, Vuononvirta J, Mertsola J, Kallonen T, He Q
- Issue date: 2012 Nov 6