The combined EURL-Salmonella interlaboratory comparison study for Food and Primary production (2017) . Detection of Salmonella in hygiene swabs
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Series/Report no.RIVM report 2018-0021
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TitleThe combined EURL-Salmonella interlaboratory comparison study for Food and Primary production (2017) . Detection of Salmonella in hygiene swabs
Translated TitleHet gecombineerde EURL-Salmonella-ringonderzoek Voedsel en Productie dieren (2017). Detectie van Salmonella in oppervlaktebemonsteringsponsjes
PubliekssamenvattingIn October 2017, a combined EURL-Salmonella interlaboratory comparison study on the detection of Salmonella in food samples and animal primary production stage was organised. In this study, hygiene swabs were chosen to be the matrix. All the laboratories involved were able to detect Salmonella in all the contaminated hygiene swab samples; they were all successful in analysing both the blank control sample and the positive control sample correctly. One laboratory made a mistake reporting the positive control negative for Salmonella and was, therefore, scored as having a 'moderate performance'. Blank hygiene swab samples, not contaminated with Salmonella, were correctly analysed as negative by almost all the laboratories. One laboratory found Salmonella present in two of the six blank samples and this was scored as a 'poor performance'. In a follow-up study this laboratory obtained good results for all samples. Participation was obligatory for all EU Member State National Reference Laboratories (NRLs) responsible for the detection of Salmonella in food samples, and voluntary for NRLs responsible for the detection of Salmonella in primary production stage samples. These latter laboratories had already participated in the compulsory EURL study for the detection of Salmonella in primary production samples which was organised in March 2017. A total of 56 NRLs participated in this study: 33 NRLs for Salmonella in Food matrices and 23 NRLs for Salmonella in Primary Production Stage matrices (PPS). The participants came from all 28 EU Member States (MS), four of the NRLs were based in third European countries and one was based in a non-European country. The EURL-Salmonella is situated at the Dutch National Institute for Public Health and the Environment (RIVM). An important task of the EURL-Salmonella is to monitor and to improve the performance of the National Reference Laboratories in Europe.
In oktober 2017 is het gecombineerde EURL-Salmonella-ringonderzoek gehouden om Salmonella aan te tonen in sponsjes die gebruikt worden om oppervlakten van te onderzoeken materialen te bemonsteren. De sponsjes zijn gebruikt, omdat ze geschikt zijn als monster voor zowel de NRL's Voedsel, als de NRL's Dieren voor de voedselproductie. Om praktische redenen zijn deze NRL's dit jaar samengevoegd. Alle deelnemers waren in staat om Salmonella in de sponsjes op te sporen. Ook hebben de laboratoria de meegestuurde controlemonsters correct geanalyseerd. Eén laboratorium heeft een fout gemaakt in de rapportage van het controlemonster waarin Salmonella was aangetroffen. Hierdoor kreeg dit laboratorium een matige score. Bijna alle laboratoria konden de monsters waar geen Salmonella aan was toegevoegd (blanco), als zodanig opsporen. Eén laboratorium vond echter Salmonella in twee van de zes blanco monsters en scoorde daardoor een onvoldoende. Dit laboratorium heeft in de herkansing wel alle monsters goed beoordeeld. Deze kwaliteitstoets is verplicht voor alle Nationale Referentie Laboratoria (NRL's) van de Europese lidstaten die ervoor verantwoordelijk zijn om Salmonella in voedsel aan te tonen; het is vrijwillig voor NRL's die Salmonella aantonen in de leefomgeving van dieren die voor de voedselproductie worden gehouden. Deze laatste laboratoria hadden in maart 2017 al deelgenomen aan het verplichte EURL-ringonderzoek naar Salmonella. In totaal hebben 56 NRL's deelgenomen: 33 NRL's om Salmonella in voedsel aan te tonen en 23 NRL's om Salmonella aan te tonen in leefomgeving voor dieren die voor de voedselproductie worden gehouden. De NRL's waren afkomstig uit alle 28 EU lidstaten, vier NRL's uit andere Europese landen en één NRL uit een niet-Europees land. Het Europese Referentielaboratorium (EURL) Salmonella is gevestigd bij het Nederlandse Rijksinstituut voor Volksgezondheid en Milieu (RIVM). Een belangrijke taak van het EURL-Salmonella is toezien op de kwaliteit van de nationale referentielaboratoria voor deze bacterie in Europa.
SponsorsEuropean Commission, Directorate-General for Health and Food Safety (DG-Sante)
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Thirteenth CRL-Salmonella interlaboratory comparison study (2008) on typing of Salmonella spp. : Dertiende CRL-Salmonella ringonderzoek (2008) voor de typering van Salmonella spp.Berk PA; Maas HME; de Pinna E; Mooijman KA; LZO; cib (Rijksinstituut voor Volksgezondheid en Milieu RIVMHealth Protection Agency HPA, 2010-11-04)The National Reference Laboratories (NRLs) of all 27 European Member States performed well on the 2008 quality control test on Salmonella typing. The 4 laboratories which repeated the test also obtained good scores. An analysis of the pooled results from all NRLs revealed that the NRLs taken as a whole were able to assign the correct name to 97 % of the strains tested. One NRL performed the test at a relatively late date and, consequently, its data could not be included in the group analysis. Since 1992, the NRLs have been required to participate in an annual quality control test, which consists of an interlaboratory comparison study for Salmonella typing. Each Member State designates a specific laboratory within their national boundaries to be responsible for the detection and identification of Salmonella strains from samples isolated from animals and/or food products. These laboratories are then referred to as the National Reference Laboratories. The performance of the NRLs is assessed annually based on their capability to correctly identify 20 Salmonella strains. NRLs from countries outside the European Union occasionally participate in these tests, and NRLs from 2 countries belonging to the European Free Trade Association (EFTA) took part in the 2008 test. The expertise of a number of NRLs was subjected to more severe testing by having not only to identify the 20 Salmonella strains of the quality control test but also to subtype (phage typing) various other Salmonella strains. As such, these laboratories received 10 strains of each of Salmonella Enteritidis and Salmonella Typhimurium. These NRLs typed 97 % of the S. Typhimurium strains correctly. The typing of S. Enteritidis strains proved to be more troublesome, with the NRLs typing 94 % of the strains correctly. The Community Reference Laboratory for Salmonella (CRL-Salmonella) organises this annual interlaboratory comparison study in cooperation with the Health Protection Agency in London, UK. The CRL-Salmonella is situated at the National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands.
Sixteenth EURL-Salmonella interlaboratory comparison study (2011) on typing of Salmonella spp. : Zestiende EURL-Salmonella ringonderzoek (2011) voor de typering van Salmonella spp.Jacobs-Reitsma WF; Pol-Hofstad IE; Maas HME; de Pinna E; Mooijman KA; LZO; cib (Rijksinstituut voor Volksgezondheid en Milieu RIVM, 2012-10-24)The 28 National Reference Laboratories (NRLs) of all 27 European Union (EU) Member States performed well on the 2011 quality control test on Salmonella typing. Two laboratories were found to require a follow-up study on their first test. Altogether, the EU-NRLs were able to assign the correct name to 97% of the strains tested. Other participants interlaboratory comparison study Salmonella Since 1992, the NRLs of the EU Member States have been required to participate in annual quality control tests, which consist of interlaboratory comparison studies on Salmonella. Laboratories from countries outside the European Union, like EU-candidate countries, occasionally participate in these tests on a voluntary basis. Eight additional laboratories participated in the current study. Two EUcandidate countries amongst these eight additional participants did not meet the criteria for good performance in the first round. One of them did not reach this goal in the follow-up study either. The other was not able to participate in the follow-up study; a follow-up study is not compulsory for non-EU laboratories. Each EU Member State designates a specific laboratory within their national boundaries to be responsible for the detection and identification of Salmonella strains from animals and/or food products. These laboratories are then referred to as the National Reference Laboratories. The performance of these NRLs on Salmonella typing is assessed annually, based on their capability to correctly identify 20 Salmonella strains. Phage typing Nine NRLs not only serotyped the 20 Salmonella strains of the quality control test, but also subtyped 20 additional strains by phage typing. For this, the laboratories received ten strains of Salmonella Enteritidis and ten strains of Salmonella Typhimurium. These NRLs typed 98% of the S. Typhimurium strains correctly. Of the S. Enteritidis strains, 88% were phage typed correctly. The European Union Reference Laboratory for Salmonella (EURL-Salmonella) organises this annual interlaboratory comparison study on typing of Salmonella in cooperation with the Health Protection Agency in London, UK. The EURLSalmonella is situated at the National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands.
Test results of Salmonella typing by the NRLs-Salmonella in the Member States of the EU and the EnterNet Laboratories - Collaborative study VI (2001) on typing of SalmonellaKorver H; Raes M; Maas HME; Ward LR; Wannet WJB; Henken AM; MGB; LIS (PHLS-Colindale/London, 2002-09-06)Test results of Salmonella sero- and phage typing and antimicrobial susceptibility testing by the National Reference Laboratories for Salmonella in the Member States of the European Union and the EnterNet Laboratories: Collaborative study VI (2001) for Salmonella. The sixth collaborative typing study for Salmonella was organised by the Community Reference Laboratory for Salmonella (CRL-Salmonella, Bilthoven, The Netherlands) in collaboration with the Public Health Laboratory Services (PHLS), London, UK. Seventeen National Reference Laboratories for Salmonella (NRLs-Salmonella) and 15 EnterNet laboratories (ENLs) participated in the study. Three of the NRLs for Salmonella are also ENLs. The results of these three NRL-ENL laboratories will only be evaluated with the NRLs for Salmonella. In total, 19 strains of the species Salmonella enterica subsp. enterica and one strain of the species Salmonella enterica subsp. arizonae were selected for serotyping and antimicrobial susceptibility testing, while 10 strains of Salmonella Typhimurium (STM) and 10 strains of Salmonella Enteritidis (SE) were selected for phage typing. In general, no problems were encountered with the typing of the O antigens. However, some laboratories had problems with typing the H antigens. Antimicrobial susceptibility testing revealed data showing that standardisation of this technique would be required to allow for comparison between laboratories. The majority of the EnterNet Laboratories and National Reference Laboratories for Salmonella did not encounter major problems with phage typing of STM and SE strains.