4th EURL-Salmonella interlaboratory comparison study Animal Feed 2018 : Detection of Salmonella in chicken feed
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Series/Report no.RIVM report 2018-0023
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Title4th EURL-Salmonella interlaboratory comparison study Animal Feed 2018 : Detection of Salmonella in chicken feed
Translated Title4e EURL-Salmonella ringonderzoek diervoeder 2018 : Detectie van Salmonella in kippenvoer
PubliekssamenvattingIn February 2018, the EURL-Salmonella organised the 4th interlaboratory comparison study on detection of Salmonella in animal feed samples. Due to problems with the chicken feed samples, it was not possible to evaluate the NRLs' performance in this study. Participation is obligatory for the National Reference Laboratories (NRLs) responsible for analysis of Salmonella in animal feed samples in the 28 EU Member States. In total, 35 NRLs participated in this study. The EURL-Salmonella is part of the Dutch National Institute for Public Health and the Environment (RIVM). The laboratories used an internationally accepted method to detect the presence of Salmonella in chicken feed samples. Each laboratory received a package with chicken feed samples contaminated with two different concentrations of Salmonella Mbandaka or no Salmonella. As in earlier studies, the chicken feed samples were artificially contaminated with a diluted culture of Salmonella at the EURL-Salmonella laboratory. The number of positive samples found in this interlaboratory comparison study was unexpectedly low. The most probable cause was the presence of unknown inhibitory substances present in the batch of chicken feed affecting the growth of Salmonella.
In februari 2018 organiseerde het EURL-Salmonella het vierde ringonderzoek om Salmonella in diervoeder aan te tonen. Door problemen met het te onderzoeken kippenvoer was het niet mogelijk de Nationale Referentie Laboratoria (NRL's) te beoordelen op hun vermogen om Salmonella in de monsters aan te tonen. Alle NRL’s van de 28 Europese lidstaten die verantwoordelijk zijn voor de opsporing van Salmonella in diervoeder, zijn verplicht om aan het onderzoek deel te nemen. In totaal namen 35 NRL’s deel. Het EURL- Salmonella is gevestigd bij het Nederlandse Rijksinstituut voor Volksgezondheid en Milieu (RIVM). Voor het ringonderzoek gebruiken de laboratoria de internationaal erkende analysemethode om Salmonella in kippenvoer aan te tonen. Elk laboratorium kreeg een pakket toegestuurd met kippenvoer dat ofwel besmet was met Salmonella Mbandaka in twee verschillende concentraties, of geen Salmonella bevatte. De monsters werden, net zoals in eerdere studies, op het laboratorium van het EURL-Salmonella kunstmatig besmet met Salmonella. Dit keer is in onverwacht weinig monsters de aanwezigheid van Salmonella aangetoond. Zeer waarschijnlijk is dit veroorzaakt doordat onbekende stoffen in de partij kippenvoer zaten die de groei van Salmonella remden.
SponsorsEuropean Commission, Directorate-General for Health and Food Safety (DG-SANTE)
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Thirteenth CRL-Salmonella interlaboratory comparison study (2008) on typing of Salmonella spp. : Dertiende CRL-Salmonella ringonderzoek (2008) voor de typering van Salmonella spp.Berk PA; Maas HME; de Pinna E; Mooijman KA; LZO; cib (Rijksinstituut voor Volksgezondheid en Milieu RIVMHealth Protection Agency HPA, 2010-11-04)The National Reference Laboratories (NRLs) of all 27 European Member States performed well on the 2008 quality control test on Salmonella typing. The 4 laboratories which repeated the test also obtained good scores. An analysis of the pooled results from all NRLs revealed that the NRLs taken as a whole were able to assign the correct name to 97 % of the strains tested. One NRL performed the test at a relatively late date and, consequently, its data could not be included in the group analysis. Since 1992, the NRLs have been required to participate in an annual quality control test, which consists of an interlaboratory comparison study for Salmonella typing. Each Member State designates a specific laboratory within their national boundaries to be responsible for the detection and identification of Salmonella strains from samples isolated from animals and/or food products. These laboratories are then referred to as the National Reference Laboratories. The performance of the NRLs is assessed annually based on their capability to correctly identify 20 Salmonella strains. NRLs from countries outside the European Union occasionally participate in these tests, and NRLs from 2 countries belonging to the European Free Trade Association (EFTA) took part in the 2008 test. The expertise of a number of NRLs was subjected to more severe testing by having not only to identify the 20 Salmonella strains of the quality control test but also to subtype (phage typing) various other Salmonella strains. As such, these laboratories received 10 strains of each of Salmonella Enteritidis and Salmonella Typhimurium. These NRLs typed 97 % of the S. Typhimurium strains correctly. The typing of S. Enteritidis strains proved to be more troublesome, with the NRLs typing 94 % of the strains correctly. The Community Reference Laboratory for Salmonella (CRL-Salmonella) organises this annual interlaboratory comparison study in cooperation with the Health Protection Agency in London, UK. The CRL-Salmonella is situated at the National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands.
Sixteenth EURL-Salmonella interlaboratory comparison study (2011) on typing of Salmonella spp. : Zestiende EURL-Salmonella ringonderzoek (2011) voor de typering van Salmonella spp.Jacobs-Reitsma WF; Pol-Hofstad IE; Maas HME; de Pinna E; Mooijman KA; LZO; cib (Rijksinstituut voor Volksgezondheid en Milieu RIVM, 2012-10-24)The 28 National Reference Laboratories (NRLs) of all 27 European Union (EU) Member States performed well on the 2011 quality control test on Salmonella typing. Two laboratories were found to require a follow-up study on their first test. Altogether, the EU-NRLs were able to assign the correct name to 97% of the strains tested. Other participants interlaboratory comparison study Salmonella Since 1992, the NRLs of the EU Member States have been required to participate in annual quality control tests, which consist of interlaboratory comparison studies on Salmonella. Laboratories from countries outside the European Union, like EU-candidate countries, occasionally participate in these tests on a voluntary basis. Eight additional laboratories participated in the current study. Two EUcandidate countries amongst these eight additional participants did not meet the criteria for good performance in the first round. One of them did not reach this goal in the follow-up study either. The other was not able to participate in the follow-up study; a follow-up study is not compulsory for non-EU laboratories. Each EU Member State designates a specific laboratory within their national boundaries to be responsible for the detection and identification of Salmonella strains from animals and/or food products. These laboratories are then referred to as the National Reference Laboratories. The performance of these NRLs on Salmonella typing is assessed annually, based on their capability to correctly identify 20 Salmonella strains. Phage typing Nine NRLs not only serotyped the 20 Salmonella strains of the quality control test, but also subtyped 20 additional strains by phage typing. For this, the laboratories received ten strains of Salmonella Enteritidis and ten strains of Salmonella Typhimurium. These NRLs typed 98% of the S. Typhimurium strains correctly. Of the S. Enteritidis strains, 88% were phage typed correctly. The European Union Reference Laboratory for Salmonella (EURL-Salmonella) organises this annual interlaboratory comparison study on typing of Salmonella in cooperation with the Health Protection Agency in London, UK. The EURLSalmonella is situated at the National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands.
Test results of Salmonella typing by the NRLs-Salmonella in the Member States of the EU and the EnterNet Laboratories - Collaborative study VI (2001) on typing of SalmonellaKorver H; Raes M; Maas HME; Ward LR; Wannet WJB; Henken AM; MGB; LIS (PHLS-Colindale/London, 2002-09-06)Test results of Salmonella sero- and phage typing and antimicrobial susceptibility testing by the National Reference Laboratories for Salmonella in the Member States of the European Union and the EnterNet Laboratories: Collaborative study VI (2001) for Salmonella. The sixth collaborative typing study for Salmonella was organised by the Community Reference Laboratory for Salmonella (CRL-Salmonella, Bilthoven, The Netherlands) in collaboration with the Public Health Laboratory Services (PHLS), London, UK. Seventeen National Reference Laboratories for Salmonella (NRLs-Salmonella) and 15 EnterNet laboratories (ENLs) participated in the study. Three of the NRLs for Salmonella are also ENLs. The results of these three NRL-ENL laboratories will only be evaluated with the NRLs for Salmonella. In total, 19 strains of the species Salmonella enterica subsp. enterica and one strain of the species Salmonella enterica subsp. arizonae were selected for serotyping and antimicrobial susceptibility testing, while 10 strains of Salmonella Typhimurium (STM) and 10 strains of Salmonella Enteritidis (SE) were selected for phage typing. In general, no problems were encountered with the typing of the O antigens. However, some laboratories had problems with typing the H antigens. Antimicrobial susceptibility testing revealed data showing that standardisation of this technique would be required to allow for comparison between laboratories. The majority of the EnterNet Laboratories and National Reference Laboratories for Salmonella did not encounter major problems with phage typing of STM and SE strains.