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dc.contributor.authorJansen, Larissa J M
dc.contributor.authorvan de Schans, Milou G M
dc.contributor.authorde Boer, Diana
dc.contributor.authorBongers, Irma E A
dc.contributor.authorSchmitt, Heike
dc.contributor.authorHoeksma, Paul
dc.contributor.authorBerendsen, Bjorn J A
dc.date.accessioned2019-03-22T09:56:12Z
dc.date.available2019-03-22T09:56:12Z
dc.date.issued2019-02-26
dc.identifier.issn1879-1298
dc.identifier.pmid30836250
dc.identifier.doi10.1016/j.chemosphere.2019.02.166
dc.identifier.urihttp://hdl.handle.net/10029/622909
dc.description.abstractThrough agricultural soil fertilization using organic manure, antibiotic residues can accumulate in the environment. In order to assess the risks of environmental pollution by veterinary drugs, monitoring of manure for antibiotic residues is necessary. As manure is a complex matrix, extraction of antibiotics proved to be challenging. In this study, 24 extraction solvents were assessed for the extraction of residues from manure representing ten antibiotics from the antibiotic classes tetracyclines, quinolones, macrolides, lincosamides and sulfonamides. Especially for the tetracyclines and quinolones the extraction solvent selection is critical, due to high fractions of non-extractable residues especially when using aqueous solvents (62-77% and 90-95% respectively when using milli-Q water). In contrast, sulfonamides can effectively be extracted with aqueous solvents. Overall, 0.125% trifluoroacetic acid in acetonitrile in combination with McIlvain-EDTA buffer proved to be the most effective extraction solvent. A longitudinal study pointed out that most antibiotics bind to solid manure particles instantaneously after addition. Trimethoprim is an exception, but because by using the optimal extraction solvent, the optimum fraction of bound residues is desorbed, this does not hamper quantitative analysis when using spiked manure quality control samples. Based on these new insights, the current in-house multi-residue LC-MS/MS method for manure analysis, containing 48 antibiotics, was revised, additionally validated and applied to 34 incurred manure samples.en_US
dc.language.isoenen_US
dc.rights
dc.subjectAntibioticsen_US
dc.subjectExtractionen_US
dc.subjectLC-MSen_US
dc.subjectManureen_US
dc.subjectNon-extractable residuesen_US
dc.titleA new extraction procedure to abate the burden of non-extractable antibiotic residues in manure.en_US
dc.typeArticleen_US
dc.identifier.journalChemosphere 2019; 224:544-53en_US
dc.source.journaltitleChemosphere


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