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dc.contributor.authorMontero, David Arturo
dc.contributor.authorCanto, Felipe Del
dc.contributor.authorVelasco, Juliana
dc.contributor.authorColello, Rocío
dc.contributor.authorPadola, Nora Lia
dc.contributor.authorSalazar, Juan Carlos
dc.contributor.authorMartin, Carla San
dc.contributor.authorOñate, Angel
dc.contributor.authorBlanco, Jorge
dc.contributor.authorRasko, David A
dc.contributor.authorContreras, Carmen
dc.contributor.authorPuente, Jose Luis
dc.contributor.authorScheutz, Flemming
dc.contributor.authorFranz, Eelco
dc.contributor.authorVidal, Roberto M
dc.date.accessioned2019-04-08T07:26:49Z
dc.date.available2019-04-08T07:26:49Z
dc.date.issued2019-01-01
dc.identifier.issn2222-1751
dc.identifier.pmid30924410
dc.identifier.doi10.1080/22221751.2019.1595985
dc.identifier.urihttp://hdl.handle.net/10029/623000
dc.description.abstractShiga toxin-producing Escherichia coli (STEC) are foodborne pathogens causing severe gastroenteritis, which may lead to hemolytic uremic syndrome. The Locus of Enterocyte Effacement (LEE), a Pathogenicity Island (PAI), is a major determinant of intestinal epithelium attachment of a group of STEC strains; however, the virulence repertoire of STEC strains lacking LEE, has not been fully characterized. The incidence of LEE-negative STEC strains has increased in several countries, highlighting the relevance of their study. In order to gain insights into the basis for the emergence of LEE-negative STEC strains, we performed a large-scale genomic analysis of 367 strains isolated worldwide from humans, animals, food and the environment. We identified uncharacterized genomic islands, including two PAIs and one Integrative Conjugative Element. Additionally, the Locus of Adhesion and Autoaggregation (LAA) was the most prevalent PAI among LEE-negative strains and we found that it contributes to colonization of the mice intestine. Our comprehensive and rigorous comparative genomic and phylogenetic analyses suggest that the accumulative acquisition of PAIs has played an important, but currently unappreciated role, in the evolution of virulence in these strains. This study provides new knowledge on the pathogenicity of LEE-negative STEC strains and identifies molecular markers for their epidemiological surveillance.en_US
dc.language.isoenen_US
dc.subjectIntegrative Conjugative Elementen_US
dc.subjectLEE-negative STECen_US
dc.subjectLocus of Adhesion and Autoaggregationen_US
dc.subjectPathogenicity Islanden_US
dc.subjectcomparative genomicsen_US
dc.titleCumulative acquisition of pathogenicity islands has shaped virulence potential and contributed to the emergence of LEE-negative Shiga toxin-producing Escherichia coli strains.en_US
dc.typeArticleen_US
dc.identifier.journalEmerg Microbes Infect 2019; 8(1):486-502en_US
dc.source.journaltitleEmerging microbes & infections


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