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dc.contributor.authorvan der Weele, Pascal
dc.contributor.authorKing, Audrey J
dc.contributor.authorMeijer, Chris J L M
dc.contributor.authorSteenbergen, Renske D M
dc.date.accessioned2019-04-26T08:35:12Z
dc.date.available2019-04-26T08:35:12Z
dc.date.issued2019-04-13
dc.identifier.issn2405-8521
dc.identifier.pmid30991124
dc.identifier.doi10.1016/j.pvr.2019.04.008
dc.identifier.urihttp://hdl.handle.net/10029/623048
dc.description.abstractRecurrent cervical intraepithelial lesions (rCIN2/3) after treatment of CIN2/3 occur in 5-15% of cases. rCIN2/3 can result from incomplete resection of CIN2/3, where the same HPV type and variant remains present. rCIN2/3 could also occur following a new infection with a different HPV variant of the same HPV type as the initial lesion. This study investigates HPV16 consensus variants in paired HPV16 positive scrapes from baseline CIN2/3 and rCIN2/3 lesions. Paired HPV16 positive cervical scrapes of women with CIN2/3 at baseline and rCIN2/3 6 or 12 months after treatment were selected for whole-genome amplification and Illumina sequencing. Sequences were compared and nucleotide changes over time were characterized. From 14 paired samples, 10 had identical consensus variants in baseline CIN2/3 and rCIN2/3. Four paired samples showed one to three nucleotide variations at recurrent disease compared to baseline. Identical or nearly identical HPV16 consensus variants were found in scrapes of paired HPV16 positive baseline CIN2/3 and rCIN2/3 lesions after treatment, suggesting no need for HPV variant analysis when the same HPV type is found in both lesions. These results argue for either incomplete excision of baseline CIN2/3 or inability of clearance of the original HPV infection.en_US
dc.language.isoenen_US
dc.subjectCINen_US
dc.subjectHPV genome variantsen_US
dc.subjectHPV16en_US
dc.subjectRecurrent infectionen_US
dc.subjectWhole-genome sequencingen_US
dc.subjectrCINen_US
dc.titleHPV16 variant analysis in primary and recurrent CIN2/3 lesions demonstrates presence of the same consensus variant.en_US
dc.typeArticleen_US
dc.identifier.journalPapillomavirus Res 2019; 7:168-72en_US
dc.source.journaltitlePapillomavirus research (Amsterdam, Netherlands)


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