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dc.contributor.authorGiannakou, Christina
dc.contributor.authorAimonen, Kukka
dc.contributor.authorBloois, Louis van
dc.contributor.authorCatalán, Julia
dc.contributor.authorGeertsma, Robert E
dc.contributor.authorGremmer, Eric R
dc.contributor.authorde Jong, Wim H
dc.contributor.authorKeizers, Peter Hj
dc.contributor.authorSchwillens, Paul Lwj
dc.contributor.authorVandebriel, Rob J
dc.contributor.authorPark, Margriet Vdz
dc.date.accessioned2019-07-30T10:05:49Z
dc.date.available2019-07-30T10:05:49Z
dc.date.issued2019-01-01
dc.identifier.issn1748-6963
dc.identifier.pmid31124759
dc.identifier.doi10.2217/nnm-2018-0339
dc.identifier.urihttp://hdl.handle.net/10029/623189
dc.description.abstractAim: Nanomaterials and nanomedicinal products tend to interfere with various commonly used assays, including regulatory required endotoxin detection methods for medicines. We developed a method to quantify endotoxin levels that is compatible with nanomaterials and nanomedicinal products. Materials & methods: The method is based on measuring endotoxin indirectly via 3-hydroxylated fatty acids of lipid-A, using Ultra High Performance Liquid Chromatography coupled with mass spectrometry. The outcome was related to results of the commonly used Limulus Amebocyte Lysate method. Results: The ultra high performance liquid chromatography coupled with mass spectrometry method has clear advantages compared with other endotoxin determination assays; particularly the absence of nanospecific interference. Conclusion: The method is sensitive, straightforward and accurate in determining and quantifying endotoxin in nanomedicinal product samples.en_US
dc.language.isoenen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectLAL assayen_US
dc.subjectfatty acidsen_US
dc.subjectinterferenceen_US
dc.subjectlipopolysaccharideen_US
dc.subjectnanomaterialsen_US
dc.subjectnanomedicineen_US
dc.titleSensitive method for endotoxin determination in nanomedicinal product samples.en_US
dc.typeArticleen_US
dc.identifier.journalNanomedicine 2019; 14(10):1231-46en_US
dc.source.journaltitleNanomedicine (London, England)


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